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Clinical Trial Details — Status: Recruiting

Administrative data

NCT number NCT06304506
Other study ID # 514-0894/23-3000
Secondary ID
Status Recruiting
Phase N/A
First received
Last updated
Start date March 15, 2024
Est. completion date December 30, 2024

Study information

Verified date March 2024
Source University of Copenhagen
Contact Jørn Wulff W Helge, PhD
Phone +4528757506
Email jhelge@sund.ku.dk
Is FDA regulated No
Health authority
Study type Interventional

Clinical Trial Summary

The experiment is a simple cross-sectional study with three groups (n=10) in each group, young and old healthy men and obese young men. The study will investigate the composition of the adipose tissue extra cellular matrix. The main questions to answer, - is there differences in the adipose tissue extracellular matrix in obese compared to young men is there differences in the adipose tissue extracellular matrix in old compared to young men


Description:

Three groups of young (n=10) and old (n=10) healthy men and obese young (n=10) men are recruited. After verbal and written information is provided signed consent is obtained. Subjects arrive at the lab overnight fasted and not having done exercise the prior day and having consumed a normal isocaloric carbohydrate rich diet. After a voiding and a 15 min rest body composition is determined by DXA. After this blood pressure is measured in the supine position in triplicate separated by 1 min. Following this a venous blood sample is obtained from a cubital vein, and kept on ice until further handling. Subsequently an adipose tissue biopsy is obtained by the Bergström technique applied with suction from an incision 3-5 cm lateral from the umbilicus. After compression and a 30 min rest, the subject perform an incremental exercise test to determine maximal fat oxidation. In brief pulmonary oxygen uptake and carbon dioxide excretion is measured using a COSMED Quark B2 on-line system during 5 min rest sitting on a cycle ergometer, and then during exercise with 3 min increments starting at 60 Watt and increasing by 35 watts. When the respiratory exchange ratio is above 1 the exercise is stopped. After a 5 min break an incremental test until voluntary exhaustion is performed to determine maximal oxygen uptake. This completes the experiment.


Recruitment information / eligibility

Status Recruiting
Enrollment 30
Est. completion date December 30, 2024
Est. primary completion date November 15, 2024
Accepts healthy volunteers Accepts Healthy Volunteers
Gender Male
Age group 18 Years to 75 Years
Eligibility Inclusion Criteria: - Healthy men aged [18,35] and BMI [20,25] - Obese men aged [18,35] and BMI [30,40] - Healthy older men aged [60,75] and BMI [20,25] Exclusion Criteria: - Present or former cardiovascular disease and chronical inflammatory disease - Consumption of medications and supplements that influence substrate use at rest and/or during exercise

Study Design


Related Conditions & MeSH terms


Intervention

Other:
Cross sectional study
Physiological testing, Sampling of blood and abdominal adipose tissue and respiratory measurements at rest and during exercise

Locations

Country Name City State
Denmark Xlab, Faculty of Health and Medical Sciences, University of Copenhagen Copenhagen

Sponsors (1)

Lead Sponsor Collaborator
University of Copenhagen

Country where clinical trial is conducted

Denmark, 

Outcome

Type Measure Description Time frame Safety issue
Primary The effect of obesity and age on adipose tissue extracellular matrix composition Adipose tissue extracellular matrix composition will be measured in abdominal subcutaneous adipose tissue sampled 3-5 cm lateral to the umbilicus in 30 male subjects; hereof 10 young, 10 old and 10 young and obese.
In brief the tissue will be delipidated and subsequently separated by DEAE chromotography (A weak anion exchange) and subsequently analysis of glycosaminoglycan disaccharide composition will be performed by MS.
The human experimental days will be done over the first 6 months of 2024. After informed consent is obtained, each participant will be tested on one day. The laboratory tissue analysis will be performed second half of 2024.
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