Vital Signs Clinical Trial
Title: The acute effect of water pipe smoking on exhaled nitric oxide (eNO) and exhaled
breath condensate (EBC) pulmonary function tests in healthy volunteers
Objectives: To evaluate the acute effect of one cession of water pipe smoking on airway
inflammation as assessed by exhaled nitric oxide (eNO) and exhaled breath condensate (EBC)
in healthy volunteers.
Design: Prospective study evaluating these parameters before and after 30 minutes of water
pipe smoking . The changes in inflammatory parameters pre and post smoking will be evaluated
blindly.
Sample size: 100 participants Participant selection: Adults subjects who regularly smoke
water pipe . Intervention: Each subject will undergo evaluation including a respiratory
questionnaire , pulmonary function tests , exhaled nitric oxide (eNO) and exhaled breath
condensate (EBC) and carboxy- hemoglobin levels . All measurements will be evaluated before
and after one cession of 30 minutes water pipe smoking
Primary outcome parameter: Change in carboxy- hemoglobin Secondary outcome parameter:Change
in peripheral eosinophils count, pulmonary function tests, change in FeNO, and in
inflammatory parameters in EBC before and after water pipe smoking
n/a
Observational Model: Case Control, Time Perspective: Prospective
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