Varicocelectomy Clinical Trial
Official title:
Role of Colored Doppler Sonography in Evaluation of Testicular Perfusion Before and After Varicocelectomy in Patients With Infertility
To evaluate the long-term effects of varicocelectomy on testicular arterial flow via Color Doppler Sonography Parameters; The arterial flow velocities (peak systolic velocity [PSV] and end diastolic velocity [EDV]) and the resistance indices against this flow (resistive index [RI] and pulsatility index [PI]) of the testicular artery.
Introduction Varicocele, an abnormal dilatation of the internal spermatic veins, is
considered the major reversible cause of male infertility. It is observed in 35%-40% of all
men with primary infertility and in up to 80% of those with secondary infertility.
The higher incidence of varicocele in men with secondary infertility suggests its progressive
nature, which might be explained by a progressive testicular dysfunction involving both
spermatogenesis and steroidogenesis. The negative impact of varicocele on spermatogenesis has
been documented by a progressive reduction in the size of testicle ipsilateral to the
varicocele.
Semen parameters improvement after varicocele treatment were first reported by Tulloch in the
mid-20th century, who performed high surgical ligation of varicose veins in 30 patients with
infertility. The author showed a marked improvement in postoperative seminal parameters in
66% of the treated subjects.
Several theories have been formulated to explain the testicular impairment caused by
varicocele, including hypoxia, autoimmunity, elevated testicular temperature, reflux of
catecholamine, and increased oxidative stress. However, none of them can completely explain
the variable modulating effect of varicocele on male fertility. Recently, the oxidative
stress theory has emerged as an important contributory factor due to findings of an
association between elevated reactive oxygen species and impaired sperm function in men with
varicocele. Additionally, reduction of oxidative stress markers has been noted after
varicocele repair.
It is hypothesized that impaired venous drainage causes an increase in venous stasis and a
decrease in arterial blood flow, thus inducing hypoxia and deficiency in testicular
microcirculation . Also, it is thought that this hypoxia could be responsible for defective
energy metabolism at mitochondrial levels, causing dysfunction of both Leydig and germinal
cells.
The arterial supply to the human testis has 3 major components: the testicular artery, the
cremasteric artery, and the vasal artery. Two-thirds of total blood is supplied by the
testicular artery. The testicular artery divides into 2 branches in the testis; the capsular
branch continues in the surface of the testis, the intratesticular branch continues in the
parenchyma and deep into the testis.
Colored Doppler Sonography is well established to illustrate microvascularity and therefore
perfusion of the testis. The arterial flow velocities (peak systolic velocity [PSV] and end
diastolic velocity [EDV]) and the resistance indices against this flow (resistive index [RI]
and pulsatility index [PI]) in the testis can be measured with this technique.
The encouraging benefits of varicocele repair to the semen parameters of subfertile men were
ratified by three recent meta-analyses:
Agarwal et al. pooled 17 studies that included only infertile men with clinical varicocele
and with at least one abnormal semen parameter. The intervention was either high ligation of
the veins or subinguinal microsurgical varicocelectomy. There was a consistent improvement in
sperm concentration and motility after both high ligation and microsurgical subinguinal
techniques, with better results after microsurgical approach. Sperm morphology change was not
stratified according to the type of intervention; nevertheless, there was an increment of
3.16% on the average of normal forms.
Baazeem et al. have also evaluated the role of varicocele repair on semen analysis. They
pooled 22 studies of various designs, including meta-analyses as well as randomized and
nonrandomized prospective controlled studies, and analyzed sperm concentration before and
after repair of clinical varicoceles in men with infertility and abnormal semen parameters.
The authors found an overall increase in sperm concentration of 12.32 × 106 ml−1 from before
to after interventions. In their study, sperm motility data were available from 17
prospective studies. Using the random-effects model a combined increment of 10.86% in
motility was demonstrated. They also evaluated five prospective studies reporting the
percentage of progressive motile sperm and confirmed that a significant improvement has
occurred as well. The pooled improvement in progressive sperm motility was 9.69% using the
random-effects model.
Finally, Schauer et al. published a meta-analysis aiming to determine the impact of each type
of intervention; namely, high ligation, inguinal varicocelectomy, and subinguinal
varicocelectomy on the semen parameters of subfertile men. The aforementioned study pooled 14
articles including randomized controlled trials, interventional trials, and cohort studies,
which accounted for 1476 subjects. Inclusion criteria comprised subfertility and/or at least
one abnormal semen parameter, clinical varicocele, and 19 years of age or older. All the
studied techniques led to a significant improvement in sperm count and motility. Only minimal
differences were observed between intervention groups. The higher increment in sperm count
and sperm motility was achieved by inguinal approaches, albeit of no clinical significance
when compared to other techniques. The results of this meta-analysis indicated that
varicocelectomy leads to significant improvements in sperm count and motility regardless of
the chosen surgical technique.
Impaired venous drainage causes an increase in venous stasis and a decrease in arterial blood
flow, thus inducing hypoxia, deficiency in testicular microcirculation and subsequent
accumulation of oxygen free radicals.
;
| Status | Clinical Trial | Phase | |
|---|---|---|---|
| Completed |
NCT03344588 -
The Role of Artery-Preserving Varicocelectomy in Subfertile Men With Severe Oligozoospermia.
|
N/A |