Urea in Blood; High Clinical Trial
Official title:
In Vitro Evaluation of Antibacterial Activity of Urea Solution Against Ocular Bacterial Isolates
Antibacterial properties of urea was tested against 35 isolates from ocular infections.
In vitro susceptibility testing was performed with commercially available antibiotic discs,
homemade urea discs saturated with urea solution at a concentration of 96 mmol. The weight of
urea in one drop = 0.37 mg, in one ml = 10.34 mg (5 microliter/ disc) discs and combined
discs (antibiotic discs to which urea solution was added). Combined discs provided better in
vitro susceptibility results than antibiotics alone.
Scanning electron micrographs of bacterial isolates incubated overnight with urea solution
showed marked disruption of bacterial aggregative patterns and irregularities of their cell
walls.
Western blotting showed marked reduction in total proteins in bacterial isolates incubated
overnight with urea solution. These findings may explain the possible mechanisms for
antibacterial activity of urea solution as a result of protein denaturation.
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