Study of the Expression of Heat Shock Proteins in Populations of B Lymphocytes in Human Tonsils

Tonsillectomy Clinical Trial

— HOTLYMPHO  

Official title:

Single-centre Study of the Expression of Heat Shock Proteins in the Lymphocytes From the Tonsils of Patients With Various Diseases Requiring Tonsillectomy

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT02859090
• Other study ID #: DUVILLARD UB 2012
• Status: Completed
• Phase: N/A
• Start date: August 26, 2014
• Est. completion date: November 20, 2014

Study information

• Verified date: January 2019
• Source: Centre Hospitalier Universitaire Dijon
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

It has recently been shown that various HSPs are strongly expressed in B lymphomas. Indeed, HSP90 stabilizes the protein Bcl-6 in diffuse large-cell B lymphomas. Pharmacological inhibition of HSP90 in vitro induced apoptosis in lymphoma cells (Cerchietti et al. Nat. Medicine, 2009, 1369-1376). In addition, HSP110 is strongly expressed in non-Hodgkin lymphomas, and targeting this HSP at the cell surface using specific antibodies could constitute a future therapy (Zappasodi et al. Blood, 2011, 4421-4430). Despite the importance of these proteins in the development of lymphomas, their expression and their role in the activation of normal B lymphocytes and the normal development of germinal centres is not known.

The expression of Heat shock proteins should vary among the different B lymphocyte populations present in the tonsils (naïve B cells, memory B cells, germinal centre B cells).

The aim of this study is to establish an expression profile for heat shock proteins in populations of B lymphocytes in human tonsils

Recruitment information / eligibility

• Status: Completed
• Enrollment: 5
• Est. completion date: November 20, 2014
• Est. primary completion date: November 20, 2014
• Accepts healthy volunteers: No
• Gender: All
• Age group: N/A and older

Eligibility

Inclusion Criteria:

• Persons who have provided oral consent

• Tonsillectomy in children or adults

Exclusion Criteria:

• Persons without national health insurance cover

• Adults under guardianship

• Pregnant or breast-feeding women

• Persons with positive HIV or HCV serology

Related Conditions & MeSH terms

• Tonsillectomy

Intervention

Procedure:
• tonsillectomy

Biological:
• blood sample

Locations

Country	Name	City	State
France	CHU Dijon Bourgogne	Dijon

Sponsors (1)

Lead Sponsor	Collaborator
Centre Hospitalier Universitaire Dijon

Country where clinical trial is conducted

France, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	expression of heat shock proteins		Day one