Stress Oxidative Clinical Trial
Official title:
Natural Antioxidant Intake After Exercise Can Reduce Malondialdehyde (MDA) Level in the Third Trimester of Pragnancy
Pregnancy is a condition that increased requirements of energy and oxygen, and if this
condition is not maximally that can lead oxidative stress and produce free radicals which is
affect in the pregnancy process. Malondialdehyde (MDA) level in pregnant women is higher than
non-pregnant women. Exercise is one way to neutralize the free radical. The aim of this study
was analyzed effect of treatment natural antioxidants after exercise which was pregnancy
workout to MDA level in the third trimester of pregnancy.
This study was a true experimental study. There were 24 prenatal pregnant women and separated
to 3 groups, which were 8 prenatal pregnant women without pregnancy workout as a control
group, 8 prenatal pregnant women with pregnancy workout but without natural antioxidant
consume as a positive control group, and 8 prenatal pregnant women with natural antioxidant
consume after pregnancy workout as a treatment group. The antioxidant compounds given in this
study were guava juice that contains vitamin C. MDA levels was analyzed by
spectrophotometric. Comparison between groups was done by one way ANOVA test.
Pregnancy is a condition that is vulnerable to all kinds - stress resulting in changes in
physiology and metabolic function. In pregnancy there is also an increase in energy and
oxygen requirements.
The metabolic process can increase require of the oxygen and if it is not available as well
as unmaximal used can causes the formation of oxidative stress and produces excessive free
radicals which ultimately, that affected in the saferity of a pregnancy process. The
imbalance between antioxidants and free radicals in pregnancy prosses are causes pathological
and complications changes in pregnancy. This kind of biochemical marker of antioxidants and
oxidants is very useful in observing complications that may arise in pregnancy.
Treatment given is expected to eliminate complaints that arise while exercise during
pregnancy can prevent the formation of free radicals, but exercise must be given in
accordance with the ability of pregnant women.
Physical exercise or exercise in pregnancy can maintain excess weight gain, prevent diabetes,
hypertension, and shorten labor time. Sports is one way to neutralize the formation of free
radicals because by doing physical activity, excessive oxygen can be used to produce energy.
Increased free radicals are characterised by an increase of malondialdehyde (MDA) as a
biochemical markers. Malondialdehyde (MDA) which is the output of lipid peroxidation prosses
that measured to determine the presence of oxidative stress due to damage by free radicals.
The resent study was found that MDA levels in pregnant women were higher compared to
non-pregnant women. Increased MDA levels are in line with the increase in gestational age,
from the first, second, and third trimesters. Study was also found that there was a
significant increase in MDA levels accompanied by a decrease in non-enzymatic antioxidants,
such as Vitamin-E, Vitamin-C, and Vitamin-A in pregnant women with hypertension. Moreover
this study assums that was "Giving Antioxidant to decrease Malondialdehyde (MDA) in the third
trimester of pregnancy". futher more the aims of this study was analyzed effect of natural
antioxidants treatment after exercise to MDA level in the third trimester of pregnancy.
This study was a experimental study. This study was located in the several clinics midwife
practice, Community Health Centers (Puskesmas) in Palembang and Bio Sains Riset Laboratory
Palembang from August to October 2016.
the study was approved informed consent from all participans as well as ethical clearance
approval number is 007/UP-ETIK/I/2016. This study was obtained 24 prenatal pregnant women
that separated to 3 groups, which were 8 prenatal pregnant women without pregnancy workout
exercise as a control group, 8 prenatal pregnant women with pregnancy workout exercise as a
positive control group and 8 prenatal pregnant women with consume natural antioxidants after
pregnancy workout exercise as a treatment group.
The antioxidant compounds given in this study were natural vitamin C from 100 ml guava juice
without additionaly sugar, 5 cc syringe, cotton, alcohol, syringe, hanscoon, EDTA tube,
serum, TCA 5%, Na thiobarbiturat 0,65%, MDA standart, and aquades.
Spectrophotometric assay used to measure the MDA levels in the Bio Sains Riset Laboratory,
Palembang and followed the essay of protocol kit from manufacture.
Comparison between groups used by one way ANOVA test, that was considered significant if p <
0.05. All statistical analyses were done by SPPS for Windows version 16.0.
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