Outcome
Type |
Measure |
Description |
Time frame |
Safety issue |
Primary |
Assessment of hemoglobin (Hb) |
Blood samples were collected between 8:00 and 9:00 a.m. after an overnight fast and time of relaxation. The athletic girls were asked to refrain from exercising the day before sample. Blood was taken from the antecubital vein and placed in one EDTA tube and one beads tube for serum separation. An automatic cell counter (Sysmex XS 1000, Japan) was used to measure hemoglobin (Hb). |
2 months |
|
Primary |
Assessment of hematocrit (Hct) |
Blood samples were collected between 8:00 and 9:00 a.m. after an overnight fast and time of relaxation. The athletic girls were asked to refrain from exercising the day before sample. Blood was taken from the antecubital vein and placed in one EDTA tube and one beads tube for serum separation. An automatic cell counter (Sysmex XS 1000, Japan) was used to measure hematocrit (Hct). |
2 months |
|
Primary |
Assessment of red blood cell (RBC) count |
Blood samples were collected between 8:00 and 9:00 a.m. after an overnight fast and time of relaxation. The athletic girls were asked to refrain from exercising the day before sample. Blood was taken from the antecubital vein and placed in one EDTA tube and one beads tube for serum separation. An automatic cell counter (Sysmex XS 1000, Japan) was used to measure red blood cell (RBC) count. |
2 months |
|
Primary |
Assessment of mean corpuscular volume (MCV) |
Blood samples were collected between 8:00 and 9:00 a.m. after an overnight fast and time of relaxation. The athletic girls were asked to refrain from exercising the day before sample. Blood was taken from the antecubital vein and placed in one EDTA tube and one beads tube for serum separation. An automatic cell counter (Sysmex XS 1000, Japan) was used to measure mean corpuscular volume (MCV). |
2 months |
|
Primary |
Assessment of mean corpuscular hemoglobin concentration (MCHC) |
Blood samples were collected between 8:00 and 9:00 a.m. after an overnight fast and time of relaxation. The athletic girls were asked to refrain from exercising the day before sample. Blood was taken from the antecubital vein and placed in one EDTA tube and one beads tube for serum separation. An automatic cell counter (Sysmex XS 1000, Japan) was used to measure mean corpuscular hemoglobin concentration (MCHC). |
2 months |
|
Primary |
Assessment of serum transferrin |
Blood samples were collected between 8:00 and 9:00 a.m. after an overnight fast and time of relaxation. The athletic girls were asked to refrain from exercising the day before sample. Blood was taken from the antecubital vein and placed in one EDTA tube and one beads tube for serum separation. The serum sample was centrifuged, and the serum transferrin was measured using COBAS Integra 400 plus, Switzerland. |
2 months |
|
Primary |
Assessment of serum ferritin |
Blood samples were collected between 8:00 and 9:00 a.m. after an overnight fast and time of relaxation. The athletic girls were asked to refrain from exercising the day before sample. Blood was taken from the antecubital vein and placed in one EDTA tube and one beads tube for serum separation. The serum sample was centrifuged, and the serum ferritin was measured using ADVIA Centaur XPT, Germany. |
2 months |
|
Secondary |
Anthropometric measures |
A weight-height scale was used to assess the weight (Kg) and height (m) for each female athlete in the two groups. The body mass index (BMI) was then calculated by dividing the weight by the height squared (Kg/m2). |
2 months |
|
Secondary |
Assessment of participation age to sport |
Each female athlete was asked about her age of participation to sport to assess her training profile. |
2 months |
|
Secondary |
Assessment of number of hours of training per week |
Each female athlete was asked about the number of hours of training per week to assess her training profile. |
2 months |
|
Secondary |
Assessment of training experience |
The training experience in years was calculated by subtracting the female athlete's age of participation to training from her chronological age. |
2 months |
|