Proteome Remodelling

Skeletal Muscle Physiology Clinical Trial

Official title:

Proteome Remodelling and Dynamics in Human Skeletal Muscle: Effect of Training

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT04136457
• Other study ID #: MiDy
• Status: Completed
• Phase: N/A
• Start date: October 30, 2019
• Est. completion date: November 28, 2023

Study information

• Verified date: December 2023
• Source: University of Copenhagen
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

The purpose of the project is to investigate the proteome signature dynamics and remodelling in human skeletal muscle induced by exercise training

Recruitment information / eligibility

• Status: Completed
• Enrollment: 72
• Est. completion date: November 28, 2023
• Est. primary completion date: November 28, 2023
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 18 Years to 45 Years

Eligibility

Inclusion Criteria:

• Healthy men and women

• VO2max > 55 mL/min/kgLM for men and > 50 for women (only applicable for endurance and sprint training interventions)

• BMI < 26

Exclusion Criteria:

• Chronic disease deemed by the medical doctor to pose a risk or to affect study outcomes

• smoking

• Use of prescription medicine deemed by the medical to affect study outcomes

• Pregnancy

Related Conditions & MeSH terms

• Skeletal Muscle Physiology

Intervention

Other:
• Endurance training
Subjects perform a period of endurance training
• Resistance training
Subjects perform a period of resistance training
• Sprint training
Subjects perform a period of sprint training

Locations

Country	Name	City	State
Denmark	August Krogh Building	Copenhagen

Sponsors (1)

Lead Sponsor	Collaborator
Morten Hostrup, PhD

Country where clinical trial is conducted

Denmark, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Other	Skeletal muscle mitochondrial supercomplexes	Pathway enrichment analysis of mitochondrial supercomplexes	Change from baseline at week 6 following endurance and sprint training
Primary	Skeletal muscle proteome signature	Pathway enrichment analysis of the muscle proteome	Change from baseline at week 6 or 8 depending on the intervention
Primary	Anabolic signaling	Western blotting of proteins related to anabolic signaling	Change in anabolic signaling from rest to 1 hour following resistance exercise both at baseline and following 8 weeks of resistance training
Secondary	Skeletal muscle mitochondrial respiration	High resolution respirometry	Change from baseline at week 6 following endurance and sprint training
Secondary	Maximal oxygen consumption	VO2max during incremental exercise	Change from baseline at week 6 following endurance and sprint training
Secondary	Body composition	Lean and fat mass during dual X-ray absorptiometry	Change from baseline at week 6 or 8 depending on the intervention
Secondary	Myonuclei	Immunohistochemistry to determine number of myonuclei	Change from baseline at week 8 following resistance training
Secondary	Satellite cells	Immunohistochemistry to determine number of satellite cells	Change from baseline at week 8 following resistance training
Secondary	Fiber size	Immunohistochemistry to determine fiber size	Change from baseline at week 8 following resistance training