Investigate the Activity of Endotoxin in Severe Sepsis

Septic Shock Clinical Trial

Official title:

Investigate the Activity of Endotoxin in Patients With Severe Sepsis and Septic Shock

Clinical Trial Details — Status: Terminated

Administrative data

• NCT number: NCT01957254
• Other study ID #: 201211040RIB
• Status: Terminated
• Phase: N/A
• First received: July 4, 2013
• Last updated: December 1, 2014
• Start date: January 2013
• Est. completion date: December 2016

Study information

• Verified date: December 2014
• Source: National Taiwan University Hospital
• Contact: n/a
• Is FDA regulated: No
• Health authority: Taiwan: Department of Health
• Study type: Observational

Clinical Trial Summary

Endotoxin is the major mediator of gram-negative bacteria which cause the systemic inflammation and result in microcirculatory dysfunction, and it leads to multiple organ dysfunction and death in patients with severe sepsis and septic shock. The goal of this study is to measure the endotoxin activity of patients with severe sepsis and septic shock at certain time points, and furthermore, to compare the difference of endotoxin activity among different pathogens, infection source, and antibiotics. The study will enroll severe sepsis and septic shock patients. The endotoxin activity will be measured at certain time points according to the protocol.

Description:

Endotoxin is the major mediator of gram-negative bacteria which cause the systemic inflammation and result in microcirculatory dysfunction, and it leads to multiple organ dysfunction and death in patients with severe sepsis and septic shock.

The goal of this study is to measure the endotoxin activity of patients with severe sepsis and septic shock at certain time points, and furthermore, to compare the difference of endotoxin activity among different pathogens, infection source, and antibiotics. The study will enroll severe sepsis and septic shock patients.

The endotoxin activity will be measured by Limulus Amebocyte Lysate (LAL) test. Limulus Amebocyte Lysate (LAL) test will be used to detect and quantify serum level of endotoxin. The critical component of the LAL reagents used in endotoxin tests is derived from blood cells (amebocytes) of the horseshoe crab, Limulus polyphemus. It contains the proteins of the blood clotting mechanism, which is triggered by endotoxins. LAL reagents are primarily used to test for endotoxins in injectable pharmaceuticals, biological products, and medical devices. They are also used in renal dialysis centers and a wide range of other applications. LAL tests are described in the Bacterial Endotoxins Test chapter in the United States Pharmacopeia (Chapter 85) and in the equivalent chapters in the European Pharmacopoeia (Chapter 2.6.14) and the Japanese Pharmacopoeia (Part I, General Tests, No. 6). We will adopt the chromogenic method as purchased from the Associates of Cape Cod Inc. (ACC). The LAL reagent is formulated with a synthetic substrate which gives a yellow color when acted upon by endotoxin activated enzyme. The test is read at 405 nm, usually in a microplate reader. The severity of multiple organ dysfunction and 28-day mortality will be followed up.

Recruitment information / eligibility

• Status: Terminated
• Enrollment: 2
• Est. completion date: December 2016
• Est. primary completion date: June 2016
• Accepts healthy volunteers: No
• Gender: Both
• Age group: 20 Years and older

Eligibility

Inclusion Criteria:

• ICU patients with new onset of severe sepsis and septic shock

• Presence of at least 2 of the following conditions (These criteria should have occurred between 12 hours before or 6 hours after the onset of the qualifying first organ dysfunction)

• Fever or hypothermia (body temperature over 38 ? or under 36 ?

• Tachycardia (heart rate > 90 bpm)

• Tachypnea (respiratory rate over 20 breaths/min or under mechanical ventilation)

• Leukocyte count more than 12,000 cells/mm3, less than 4,000 cells/mm3, or more than 10 % of immature form (band)

Exclusion Criteria:

Patient will be excluded if they

• are under 20 years old or older than 99 years old

• have suffered from severe sepsis or septic shock more than 24 hours

• are pregnant

• were treated with another medicine or device in the trial less than 30 days prior to the admission to this trial

• have received organ transplantation less than 1 years prior to this trial

• are terminally ill, for examples with metastasis, with a life expectancy of less than 30 days (certified by the attending physician)

• have already received other blood cleaning treatments, such as CVVH, HD, HF, and PE upon entry into the trial

• have chosen palliative care and signed Do Not Resuscitate sheet

• non-native speaker

Study Design

Time Perspective: Prospective

Related Conditions & MeSH terms

• Sepsis
• Septic Shock
• Severe Sepsis
• Shock, Septic

Locations

Country	Name	City	State
Taiwan	National Taiwan University Hospital	Taipei

Sponsors (1)

Lead Sponsor	Collaborator
National Taiwan University Hospital

Country where clinical trial is conducted

Taiwan, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Endotoxin activity	The endotoxin activity will be measured by Limulus Amebocyte Lysate (LAL) test. Limulus Amebocyte Lysate (LAL) test will be used to detect and quantify serum level of endotoxin.	baseline	No
Secondary	Change of endotoxin activity	Compare the change of endotoxin activity at different time points.	At enrollment, 24h, 48h, and 72h	No