Distribution of Nutrient Derived Amino Acids

Sarcopenia Clinical Trial

Official title:

Distribution of Nutrient Derived Amino Acids and Anabolic Effect in Healthy Elderly Individuals

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT03870425
• Other study ID #: BBH 140
• Status: Completed
• Phase: N/A
• Start date: April 9, 2019
• Est. completion date: February 28, 2022

Study information

• Verified date: November 2022
• Source: Bispebjerg Hospital
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

The project examines over several days how the distribution pattern of dietary protein stimulate and affect the protein turnover of important proteins in the aging perspective, such as skeletal muscle proteins.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 24
• Est. completion date: February 28, 2022
• Est. primary completion date: June 30, 2020
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 65 Years to 80 Years

Eligibility

Inclusion Criteria:

• Men and women

• Between 65-80 years of age

Exclusion Criteria:

• BMI above 30

• Smokers

• Vegetarians

• Diabetes or other metabolic diseases

• Medical treatment which affects protein turnover

• Gastro-intestinal diseases, reduced kidney or liver function, or chronic inflammation diseases

• Elevated blood-pressure or signs of atherosclerosis

• More than 5 hours of regular exercise each week

Related Conditions & MeSH terms

• Diet Modification
• Muscle Mass
• Protein Breakdown
• Protein Intake
• Protein Synthesis
• Sarcopenia

Intervention

Other:
• Distribution pattern of dietary proteins (minced meat)
Study effect of an even or skewed distribution pattern of dietary protein

Locations

Country	Name	City	State
Denmark	Institute of Sports Medicine Copenhagen	Copenhagen

Sponsors (1)

Lead Sponsor	Collaborator
Bispebjerg Hospital

Country where clinical trial is conducted

Denmark, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Muscle protein synthesis	Myofibrillar FSR measured by deuterated water technique, by which deuterium labeled alanine incorporation into myofibrillar muscle protein synthesis can be measured.	3 days
Primary	Net protein balance	Whole body net protein balance measured during 10 hours acute study. Measured by the application of stable amino acid labeled phenylalanine and tyrosine tracers, by which the protein synthesis, phenylalanine oxidation and protein breakdown can be calculated.	10 hours
Secondary	D5-phenylalanine incorporation	D5-phenylalanine incorporation into myofibrillar proteins. Dietary meat proteins are intrinsically labeled with D5-phenylalanine	3 days
Secondary	Muscle protein synthesis	Myofibrillar protein synthesis (%/hour) measured during 10 hours of the acute study. Calculated from the difference in D8-phenylalanine incorporation from the muscle biopsies taken at 2 hours and 12 hours into the stable amino acid tracer infusion.	10 hours
Secondary	Protein synthesis	Whole body protein synthesis (%/hour) measured during acute study, by the use of stabel amino acid tracer technique.	10 hours
Secondary	Protein breakdown	Whole body protein breakdown (%/hour) measured during acute study, by the use of stabel amino acid tracer technique.	10 hours
Secondary	Phenylalanine oxidation	Phenylalanine oxidation measured during acute study	10 hours
Secondary	D5-phenylalanine appearance	Plasma D5-phenylalanine enrichment after intake of D5-phenylalanine intrinsically labelled meat proteins	12 hours
Secondary	Signaling pathways in skeletal muscle	Intracellular signaling pathways related to protein turnover. Measured by Western blotting technique from the muscle tissue biopsies taken at 2 hours and 12 hours into the acute study.	10 hours
Secondary	Plasma insulin	Plasma insulin concentration during acute study	12 hours