Microbial Dysbiosis in Rheumatoid Arthritis

Rheumatoid Arthritis Clinical Trial

— MyRA  

Official title:

Microbial Dysbiosis in the Pathogenesis of Rheumatoid Arthritis: Using Metagenomics to Predict Methotrexate Efficacy

Clinical Trial Details — Status: Enrolling by invitation

Administrative data

• NCT number: NCT03802890
• Other study ID #: QIB01/2018
• Status: Enrolling by invitation
• Start date: February 1, 2019
• Est. completion date: July 30, 2021

Study information

• Verified date: June 2020
• Source: Quadram Institute Bioscience
• Contact: n/a
• Is FDA regulated: No
• Study type: Observational

Clinical Trial Summary

The MyRA study will primarily investigate whether there are associations between the structure and function of the gut microbiome and response to methotrexate in early rheumatoid arthritis patients. The microbiome will be characterised via shotgun metagenomic sequencing of microbial DNA present in stool samples taken during the participant's first 6 months of taking methotrexate.

Description:

Methotrexate is often the first drug of choice for patients with early rheumatoid arthritis (RA), but its efficacy is highly variable and it can lead to severe side effects. There are currently no reliable predictors of methotrexate efficacy for people with early RA.

Microbial dysbiosis (an imbalanced microbiome) has recently been implicated in RA, with associations between specific microbes and RA biomarkers or disease activity. Gut microbes have extensive capabilities in terms of xenobiotic (e.g. drug) metabolism. Several gut microbes are able to alter the drug methotrexate in vitro, and it is possible this could effect drug efficacy in vivo. Alternatively methotrexate efficacy could be affected by baseline microbial composition or alterations to microbial composition over the course of treatment.

Recruitment information / eligibility

• Status: Enrolling by invitation
• Enrollment: 30
• Est. completion date: July 30, 2021
• Est. primary completion date: April 30, 2021
• Accepts healthy volunteers: No
• Gender: All
• Age group: 18 Years to 65 Years

Eligibility

Inclusion Criteria:

• 18-65 years of age

• RA diagnosis based on ACR 2010 classification criteria with symptoms starting within the last 2 years

• Referred by GP to the Early Arthritis Clinic at the Norfolk and Norwich University Hospitals NHS trust

• Commencing methotrexate monotherapy for the first time

Exclusion Criteria:

• Initially commencing combination therapy (prior to first stool sample) rather than methotrexate monotherapy i.e. MTX combined with another DMARD or prednisolone

• Commencement of MTX therapy prior to first stool sample or cessation of MTX therapy at any point during the study

• History of psoriasis

• Those currently suffering from, or have ever suffered from, any diagnosed gastrointestinal disease, gastrointestinal disorders including regular diarrhoea and constipation (excluding hiatus hernia unless symptomatic) and/or have undergone gastrointestinal surgery.

• Those regularly (3+ times/week) taking self-prescribed over the counter medications for digestive/gastrointestinal conditions

• Use of laxatives within 7 days prior to sampling unless these have been used on a regular basis (3+ times/week) for more than one month prior to the study and will continue to be used throughout the study period

• The use of over-the-counter medications or food/drinks containing pre and/or probiotics within 7 days prior to sampling, unless these have been used on a regular basis (3+ times/week) for more than one month prior to the study and will continue to be used throughout the study period

• Significant alteration of the participant's normal diet at any point during the study (e.g. adoption of the 5:2 fasting diet)

• Regular (3+ times/week) or recent (within 3 months) use of colonic irrigation or other bowel cleansing techniques

• Recently returned to the UK following a period abroad, and who have suffered gastric symptoms during the period abroad or on return to the UK. These will be assessed on an individual basis

• Currently taking or finished a course of antibiotics within the last 3 months

• Currently pregnant or lactating

• Living with or related to any member of the Study Team

• Those who have limited or no understanding of spoken and written English

Related Conditions & MeSH terms

• Arthritis
• Arthritis, Rheumatoid
• Dysbiosis
• Rheumatoid Arthritis

Locations

Country	Name	City	State
United Kingdom	Norfolk and Norwich University Hospitals NHS Foundation Trust	Norwich	Norfolk

Sponsors (4)

Lead Sponsor	Collaborator
Quadram Institute Bioscience	Action Arthritis, Norfolk and Norwich University Hospitals NHS Foundation Trust, University of East Anglia

Country where clinical trial is conducted

United Kingdom, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Change in DAS28-CRP score	Disease Activity Score using 28 joints and C-reactive Protein	0-6 months
Secondary	Change in SDAI score	Simplified Disease Activity Index	0-6 months
Secondary	Concentration of CRP in blood	C-reactive Protein (an inflammatory biomarker)	0-6 months
Secondary	ESR value (blood)	Erythrocyte Sedimentation Rate (an inflammatory biomarker)	0-6 months
Secondary	Concentration of anti-CCP in blood	Anti-Cyclic Citrullinated peptide (disease-specific antibody)	0-6 months
Secondary	Concentration of RF in blood	Rheumatoid Factor (disease-specific antibody)	0-6 months