Respiratory System Clinical Trial
Official title:
Project A3sc - An Atlas of Airways at a Single Cell Level
The increasing incidence of chronic respiratory diseases is a public health problem affecting
hundreds of thousands of people around the world, including children. Directly exposed to
atmospheric aerocontaminants (pollution, allergens), the respiratory tracts represent a
complex ecosystem that involves different cells that develop complex interactions with the
surrounding connective tissue but also with their rich immune environment and with the
microbiota. Although a pathophysiological continuum is postulated between the nasal and
bronchial airways in certain diseases, such as allergic diseases, we have identified broad
gradients in gene expression between nasal and bronchial samples. This is why cellular
variability throughout the respiratory tree needs to be studied in detail.
The sequencing of RNAs specifically present in a particular cell, and its comparison with
neighboring cells, allows us to document precise cellular contributions and intercellular
relationships. Our project will establish protocols to stabilize airway swabs by brushing
and/or biopsy, under conditions that will then allow the analysis of gene expression profiles
at the single cell level (single cell RNA sequencing).
The development of the "single cell" stabilisation and analysis protocol will first be
carried out on primary respiratory epithelium cultures and then extended to respiratory
specimens taken from healthy volunteers. Through sampling at several levels of the
respiratory tree, variations in expression along the tracheobronchial axis will be fully
documented. Finally, the interaction between the epithelial compartment and the immunological
compartment will be studied by analyzing gene expression on a single cell in different
physiopathological contexts.
n/a
| Status | Clinical Trial | Phase | |
|---|---|---|---|
| Completed |
NCT03697499 -
Effects of Fish Oil in Alleviating Health Hazards Associated With Ozone Exposure
|
N/A | |
| Active, not recruiting |
NCT04153539 -
Acute Health Effects of Traffic-Related Air Pollution Exposure
|
N/A | |
| Completed |
NCT04346576 -
Probiotics Prevent Health Problems in Children
|
N/A | |
| Active, not recruiting |
NCT05575752 -
Acute Health Effects of High Temperature Exposure
|
N/A | |
| Not yet recruiting |
NCT06076629 -
Acute Health Effects of Low Temperature Exposure
|
N/A | |
| Completed |
NCT03151941 -
Respiratory Monitoring of Intrathecal Baclofen- a Feasibility Study
|
N/A | |
| Completed |
NCT04204291 -
Project A4sc - An Atlas of Airways at a Single Cell Level
|
N/A | |
| Completed |
NCT01538667 -
Study to Characterize Lung Deposition, Pharmacokinetics, Safety and Tolerability of Single Inhalations of Radiolabeled Ciprofloxacin Dry Powder in Healthy Subjects and Patients With Chronic Lung Diseases
|
Phase 1 |