Moderate Intensity Exercise and Phenylketonuria

Phenylketonuria Clinical Trial

Official title:

The Effects of an Acute Bout of Moderate Intensity Exercise on Plasma Amino Acids in Subjects With Phenylketonuria

Clinical Trial Details — Status: Terminated

Administrative data

• NCT number: NCT01904708
• Other study ID #: 9202
• Status: Terminated
• Phase: N/A
• First received: May 31, 2013
• Last updated: October 15, 2014
• Start date: May 2013
• Est. completion date: June 2014

Study information

• Verified date: October 2014
• Source: Oregon Health and Science University
• Contact: n/a
• Is FDA regulated: No
• Health authority: United States: Institutional Review Board
• Study type: Interventional

Clinical Trial Summary

Phenylketonuria (PKU), an inherited genetic disorder, can cause irreversible brain damage, declined executive function, and autistic tendencies unless a phenylalanine (Phe) restricted diet is consistently maintained throughout life. Promoting anabolism, the uptake of free amino acids from the extracellular space, is a key component to maintaining plasma phenylalanine concentrations within treatment range among patients with PKU. Exercise promotes muscle protein synthesis and anabolism, but the effect on blood phenylalanine concentrations in patients with PKU has not been reported.

Our objective is to assess the impact of an acute bout of moderate intensity exercise on protein oxidation and plasma amino acid concentrations, as a potential adjunctive therapy for patients with PKU.

The investigators hypothesize that moderate intensity exercise decreases amino acid oxidation, increases muscle protein synthesis, and promotes tissue uptake of essential amino acids, thereby lowering plasma phenylalanine concentrations in patients with Phenylketonuria.

Description:

The indicator amino acid oxidation technique utilizes a carbon labeled isotope (L-[13C]) tracer that is ingested orally and is measured in expired breath. This method is based on the theory that if one essential amino acid is deficient, all other amino acids will be oxidized until a break-point is reached and at that time all amino acids will be incorporated into muscle protein synthesis. Using a randomized crossover design the investigators plan to investigate the effects of an acute bout of moderate intensity treadmill exercise versus sedentary activities on plasma amino acids in four subjects with PKU. The long-term goal of this novel research is to determine if exercise could be used as an adjunctive therapy to improve the management of plasma phenylalanine levels and promote a normal, healthy quality of life among patients with PKU.

Patient Recruitment: Four post-pubertal participants ranging from 14-17 years of age with classical phenylketonuria (PKU), treated at Oregon Health & Science University, will participate in this pilot study. Participants will be recruited during their routine clinic visit at Doernbecher Children's Hospital Metabolic clinic.

Study Design: This randomized crossover clinical trial will compare the effects of moderate intensity exercise and sedentary activities on amino acid oxidation and plasma amino acid pools using the indicator amino acid oxidation technique. Each participant will be studied on two separate occasions over a one-month period.

Recruitment information / eligibility

• Status: Terminated
• Enrollment: 2
• Est. completion date: June 2014
• Est. primary completion date: June 2014
• Accepts healthy volunteers: No
• Gender: Male
• Age group: 14 Years to 17 Years

Eligibility

Inclusion Criteria:

• plasma phenylalanine concentration of >1200 micromols at diagnosis

• treated by dietary phenylalanine restriction from early infancy

• willing to participate in this study

• stable BMI of 20-25 for the past 3 months

• currently in average to good physical fitness condition

• physically active 3-4 days per week

Exclusion Criteria:

• previously or are currently taking approved or experimental pharmacologic treatments (i.e.: Biopterin (BH4), sapropterin dihydrochloride (Kuvan), Large Neutral Amino Acids (LNAA), pegylated phenylalanine ammonia lyase (PEG-PAL))

• recent history of weight loss

• endocrine disorder

• pregnant

• anemic

• involved in any other study or research protocol

• participation in a current strength training or performance training regimen

Study Design

Allocation: Randomized, Endpoint Classification: Pharmacokinetics/Dynamics Study, Intervention Model: Crossover Assignment, Masking: Open Label, Primary Purpose: Basic Science

Related Conditions & MeSH terms

• Phenylketonuria
• Phenylketonurias
• PKU

Intervention

Other:
• Acute Moderate Intensity Exercise
Moderate intensity exercise by walking on a treadmill at 75% of max heart rate.

Locations

Country	Name	City	State
United States	Oregon Health and Science University	Portland	Oregon

Sponsors (1)

Lead Sponsor	Collaborator
Oregon Health and Science University

Country where clinical trial is conducted

United States, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Compare plasma phenylalanine concentration of final blood draw during exercise versus sedentary visits.	Compare the final blood sample for phenylalanine concentration from each arm of the study.	up to 8 hours	No
Secondary	Breath Enrichment of C13-Lysine during sedentary and exercise	Compare the AUC total breath enrichment of C13-Lysine during the sedentary and exercise arms.	Hours 1-8 of the study day from each arm.	No