ESWT on Human Gingival Fibroblasts

Periodontal Attachment Loss Clinical Trial

— ESWT  

Official title:

Application of Extracorporeal Shockwaves on Human Gingival Fibroblasts in Vitro Study

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT05766293
• Other study ID #: KCDS/48
• Status: Completed
• Phase: N/A
• Start date: August 22, 2022
• Est. completion date: January 10, 2023

Study information

• Verified date: March 2023
• Source: Krishnadevaraya College of Dental Sciences & Hospital
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

The idea of treating different deformities or diseases in the maxillofacial region with Extracorporeal Shockwave Therapy (ESWT) has recently become popular. Shockwave therapy was first introduced in Germany in 1980s and has been widely used in medical practice for the management of urolithiasis, cholelithiasis and in head and neck region for sialolithiasis.

The present study 'Application of Extracorporeal Shock Wave Therapy on human gingival derived fibroblasts In-vitro study' was done to explore and evaluate the effect of shockwaves. Further, these cells were assessed for Cell - Cell interaction and Cell Viability.

In this in-vitro study, 20 fibroblast cells were included. 10 samples were considered for Control group and the remaining 10 samples were considered as Test group. Extracorporeal shockwave was applied to the Test samples. They were further analysed

Description:

BACKGROUND:

Improved understanding of human biology at the molecular level, scientific and technologic advances are emerging aspects in dentistry. The idea of treating different deformities or diseases in the maxillofacial region with Extracorporeal Shockwave Therapy (ESWT) has recently become popular. Shockwave therapy was first introduced in Germany in 1980s and has been widely used in medical practice for the management of urolithiasis, cholelithiasis and in head and neck region for sialolithiasis.

AIM:

The present study 'Application of Extracorporeal Shock Wave Therapy on human gingival tissue derived fibroblasts In-vitro study' was done to explore and evaluate the effect of shockwaves. Further, fibroblast cells were assessed for Cell - Cell interaction and Cell Viability.

MATERIALS AND METHODS:

In this in-vitro study, 20 fibroblast cells were included. 10 samples were considered for Control group and the remaining 10 samples were considered as Test group. Extracorporeal shockwave was applied to the Test samples. They were further analysed using wound scratch assay which was used to assess Cell proliferative rate and scratch closure through live imaging. MTT assay and ATP assay was used to determine Cell viability after exposure to shockwaves.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 20
• Est. completion date: January 10, 2023
• Est. primary completion date: November 17, 2022
• Accepts healthy volunteers: No
• Gender: All
• Age group: N/A and older

Eligibility

Inclusion Criteria:

• Viable human gingival fibroblast

• Healthy gingival tissue

Exclusion Criteria:

• contaminated samples

Related Conditions & MeSH terms

• Periodontal Attachment Loss

Intervention

Device:
• extracorporeal shockwave device
Fibroblast cell lines (n=10) were subjected to Shockwaves from extracorporeal shockwave device

Other:
• Fibroblast cell lines not subjected to shockwaves from extracorporeal shockwave device
Fibroblast cell lines (n=10) without shockwave exposure

Locations

Country	Name	City	State
India	Krishnadevaraya College of Dental Science and Hospital	Bengaluru	Karnataka

Sponsors (1)

Lead Sponsor	Collaborator
Dr Prabhuji MLV

Country where clinical trial is conducted

India, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Fibroblast cell lines exposed to shockwave from extracorporeal shockwave device	Cell proliferation and Cell viability	74 hrs