Ovarian Reserve Clinical Trial
— GJZLLCCBGNXJOfficial title:
Clinical Study of Liver Meridian in the Treatment of Male Impotence and Female Premature Ovarian Failure
Diminished ovarian reserve (DOR) refers to a decrease in the number and quality of oocytes in the ovary, which results in impaired ovarian function and decreased fertility. Meanwhile, levels of the anti-Müllerian hormone (AMH), antral follicle count (AFC), and Follicle-Stimulating Hormone (FSH) also decrease in patients with DOR. In general, there is a decline in fertility and premature menopause. Some patients will have low menstrual volume, oligomenorrhea or even amenorrhea, abnormal uterine bleeding, ovulation disorders, infertility and perimenopausal performance before the age of 40, and eventually develop into premature ovarian failure. In recent years, with the changes of social culture, living environment, work pressure and other factors, the incidence of this disease has increased year by year, which has a great impact on women's fertility, mental health, quality of life, family relations and other aspects. As a green and safe complementary and alternative therapy, acupuncture has been proved to be effective. According to the statistics, 904 (33.54%) of the 2695 syndromes indicated by acupoints of the liver meridian recorded in 93 ancient medical books are reproductive disorders, ranking first in the diseases indicated by the liver meridian and the 14 meridians. The body surface course of the liver meridian is closely related to the genitals, and there is a close relationship between the liver meridian and the genitals in physiology and pathology. According to the theory of the relationship between meridians and zangfu organs, the study aims to verify the efficacy and safety of acupuncture of the liver meridian and provide high-level research evidence for meridian syndrome differentiation of reproductive system diseases via "treatment from the liver."
Status | Recruiting |
Enrollment | 198 |
Est. completion date | December 31, 2023 |
Est. primary completion date | December 31, 2023 |
Accepts healthy volunteers | No |
Gender | Female |
Age group | 18 Years to 40 Years |
Eligibility | Inclusion Criteria: - The inclusion criteria are as follows: 1. patients with the detection indicators within 3 months before enrollment met the diagnostic criteria of DOR. 2. patients aged between 18 and 40 years old. 3. patients who have received no other treatment in the 2 months before enrollment. (4) patients who voluntarily participate in this study and can actively cooperate with the study process. (5) patients who have signed an informed consent form. Patients who meet all five criteria will be eligible for this study. Exclusion Criteria: - (1) Patients with polycystic ovarian syndrome, hyperprolactinemia, pituitary or hypothalamic amenorrhea, thyroid dysfunction, congenital malformations, and organic lesions in the reproductive organs. (2) Patients with a history of local ovarian surgery, such as oophorectomy and ovarian tumor surgery. (3) Patients with a history of chemotherapy or pelvic radiotherapy and a history of therapy with immunosuppressive agents. (4) Patients with comorbidities in the cardiovascular system, liver, kidney, hematopoietic system, nervous and mental system, diabetes, hypertension, and other diseases. (5) Patients being administered estrogen, progesterone, or dehydroepiandrosterone (DHEA) within 2 months before enrollment. (6) Patients who cannot tolerate blood or ultrasound testing. (vii) Patients who are also participating in other clinical trials. (7) Patients who refuse to sign the informed consent form. Patients who meet any of the above criteria will be excluded. |
Country | Name | City | State |
---|---|---|---|
China | The First Affiliated Hospital of Hunan University of Chinese Medicine | Changsha | Hunan |
Lead Sponsor | Collaborator |
---|---|
The First Affiliated Hospital of Hunan University of Traditional Chinese Medicine |
China,
Atasever M, Soyman Z, Demirel E, Gencdal S, Kelekci S. Diminished ovarian reserve: is it a neglected cause in the assessment of recurrent miscarriage? A cohort study. Fertil Steril. 2016 May;105(5):1236-1240. doi: 10.1016/j.fertnstert.2016.01.001. Epub 2016 Jan 21. — View Citation
Birch S, Lee MS, Kim TH, Alraek T. On defining acupuncture and its techniques: A commentary on the problem of sham. Integr Med Res. 2022 Jun;11(2):100834. doi: 10.1016/j.imr.2022.100834. Epub 2022 Jan 8. No abstract available. — View Citation
Brinkhaus B, Hummelsberger J, Kohnen R, Seufert J, Hempen CH, Leonhardy H, Nogel R, Joos S, Hahn E, Schuppan D. Acupuncture and Chinese herbal medicine in the treatment of patients with seasonal allergic rhinitis: a randomized-controlled clinical trial. Allergy. 2004 Sep;59(9):953-60. doi: 10.1111/j.1398-9995.2004.00540.x. — View Citation
Bunnewell SJ, Honess ER, Karia AM, Keay SD, Al Wattar BH, Quenby S. Diminished ovarian reserve in recurrent pregnancy loss: a systematic review and meta-analysis. Fertil Steril. 2020 Apr;113(4):818-827.e3. doi: 10.1016/j.fertnstert.2019.11.014. Epub 2020 Mar 4. — View Citation
Cohen J, Chabbert-Buffet N, Darai E. Diminished ovarian reserve, premature ovarian failure, poor ovarian responder--a plea for universal definitions. J Assist Reprod Genet. 2015 Dec;32(12):1709-12. doi: 10.1007/s10815-015-0595-y. Epub 2015 Oct 13. — View Citation
di Clemente N, Racine C, Pierre A, Taieb J. Anti-Mullerian Hormone in Female Reproduction. Endocr Rev. 2021 Nov 16;42(6):753-782. doi: 10.1210/endrev/bnab012. — View Citation
Hilditch JR, Lewis J, Peter A, van Maris B, Ross A, Franssen E, Guyatt GH, Norton PG, Dunn E. A menopause-specific quality of life questionnaire: development and psychometric properties. Maturitas. 1996 Jul;24(3):161-75. doi: 10.1016/s0378-5122(96)82006-8. Erratum In: Maturitas 1996 Nov;25(3):231. — View Citation
Hong L, Peng S, Li Y, Fang Y, Wang Q, Klausen C, Yin C, Wang S, Leung PCK, Yang X. miR-106a Increases Granulosa Cell Viability and Is Downregulated in Women With Diminished Ovarian Reserve. J Clin Endocrinol Metab. 2018 Jun 1;103(6):2157-2166. doi: 10.1210/jc.2017-02344. — View Citation
Liu CZ, Xie JP, Wang LP, Liu YQ, Song JS, Chen YY, Shi GX, Zhou W, Gao SZ, Li SL, Xing JM, Ma LX, Wang YX, Zhu J, Liu JP. A randomized controlled trial of single point acupuncture in primary dysmenorrhea. Pain Med. 2014 Jun;15(6):910-20. doi: 10.1111/pme.12392. Epub 2014 Mar 17. — View Citation
Liu L, Pang R, Sun W, Wu M, Qu P, Lu C, Wang L. Functional social support, psychological capital, and depressive and anxiety symptoms among people living with HIV/AIDS employed full-time. BMC Psychiatry. 2013 Dec 1;13:324. doi: 10.1186/1471-244X-13-324. — View Citation
Maioli C, Falciati L, Marangon M, Perini S, Losio A. Short- and long-term modulation of upper limb motor-evoked potentials induced by acupuncture. Eur J Neurosci. 2006 Apr;23(7):1931-8. doi: 10.1111/j.1460-9568.2006.04698.x. — View Citation
Practice Committee of the American Society for Reproductive Medicine. Electronic address: asrm@asrm.org; Practice Committee of the American Society for Reproductive Medicine. Testing and interpreting measures of ovarian reserve: a committee opinion. Fertil Steril. 2020 Dec;114(6):1151-1157. doi: 10.1016/j.fertnstert.2020.09.134. — View Citation
Steiner AZ, Pritchard D, Stanczyk FZ, Kesner JS, Meadows JW, Herring AH, Baird DD. Association Between Biomarkers of Ovarian Reserve and Infertility Among Older Women of Reproductive Age. JAMA. 2017 Oct 10;318(14):1367-1376. doi: 10.1001/jama.2017.14588. — View Citation
Sun W, Stegmann BJ, Henne M, Catherino WH, Segars JH. A new approach to ovarian reserve testing. Fertil Steril. 2008 Dec;90(6):2196-202. doi: 10.1016/j.fertnstert.2007.10.080. Epub 2008 Apr 22. — View Citation
Sun XY, Lan YZ, Liu S, Long XP, Mao XG, Liu L. Relationship Between Anti-Mullerian Hormone and In Vitro Fertilization-Embryo Transfer in Clinical Pregnancy. Front Endocrinol (Lausanne). 2020 Dec 4;11:595448. doi: 10.3389/fendo.2020.595448. eCollection 2020. — View Citation
Wang Y, Li Y, Chen R, Cui X, Yu J, Liu Z. Electroacupuncture for reproductive hormone levels in patients with diminished ovarian reserve: a prospective observational study. Acupunct Med. 2016 Oct;34(5):386-391. doi: 10.1136/acupmed-2015-011014. Epub 2016 May 13. — View Citation
Zhao L, Chen J, Li Y, Sun X, Chang X, Zheng H, Gong B, Huang Y, Yang M, Wu X, Li X, Liang F. The Long-term Effect of Acupuncture for Migraine Prophylaxis: A Randomized Clinical Trial. JAMA Intern Med. 2017 Apr 1;177(4):508-515. doi: 10.1001/jamainternmed.2016.9378. — View Citation
Zung WW. A rating instrument for anxiety disorders. Psychosomatics. 1971 Nov-Dec;12(6):371-9. doi: 10.1016/S0033-3182(71)71479-0. No abstract available. — View Citation
* Note: There are 18 references in all — Click here to view all references
Type | Measure | Description | Time frame | Safety issue |
---|---|---|---|---|
Other | White blood cell (WBC) | Normal value (unit): 3.5-9.5 (109/L). Assessment with reference to the normal range. 1- Normal (Within the reference range) 2- Abnormal (Outside the reference range). The purpose is to evaluate whether acupuncture has an effect on WBC, which is a safety assessment. | Baseline period(1 week before enrollment), enrollment 12 weeks (Change from Baseline at 12weeks). | |
Other | Red blood cell (RBC) | Normal value (unit): 3.8-5.1(1012/L). Assessment with reference to the normal range. 1- Normal (Within the reference range) 2- Abnormal (Outside the reference range). The purpose is to evaluate whether acupuncture has an effect on RBC, which is a safety assessment. | Baseline period(1 week before enrollment), enrollment 12 weeks (Change from Baseline at 12weeks) | |
Other | Blood platelet | Normal value (unit): 125-350(109/L). Assessment with reference to the normal range. 1- Normal (Within the reference range) 2- Abnormal (Outside the reference range). The purpose is to evaluate whether acupuncture has an effect on Blood platelet, which is a safety assessment. | Baseline period(1 week before enrollment), enrollment 12 weeks (Change from Baseline at 12weeks) | |
Other | Hemoglobin | Normal value (unit): 115-150 (g/L). Assessment with reference to the normal range. 1- Normal (Within the reference range) 2- Abnormal (Outside the reference range). The purpose is to evaluate whether acupuncture has an effect on Hemoglobin, which is a safety assessment. | Baseline period(1 week before enrollment), enrollment 12 weeks (Change from Baseline at 12weeks) | |
Other | Alanine aminotransferase(ALT) | Normal value (unit): 0-33(IU/L). Assessment with reference to the normal range. 1- Normal (Within the reference range) 2- Abnormal (Outside the reference range). The purpose is to evaluate whether acupuncture has an effect on ALT, which is a safety assessment. | Baseline period(1 week before enrollment), enrollment 12 weeks (Change from Baseline at 12weeks) | |
Other | Aspartate aminotransferase (AST) | Normal value (unit): 0-32(IU/L). Assessment with reference to the normal range. 1- Normal (Within the reference range) 2- Abnormal (Outside the reference range). The purpose is to evaluate whether acupuncture has an effect on AST, which is a safety assessment. | Baseline period(1 week before enrollment), enrollment 12 weeks (Change from Baseline at 12weeks) | |
Other | Blood - urea nitrogen (UREA) | Normal value (unit): 2.14-7.14 (umol/L). Assessment with reference to the normal range. 1- Normal (Within the reference range) 2- Abnormal (Outside the reference range). The purpose is to evaluate whether acupuncture has an effect on UREA, which is a safety assessment. | Baseline period(1 week before enrollment), enrollment 12 weeks (Change from Baseline at 12weeks) | |
Other | Creatinine (CREA) | Normal value (unit): 45-84 (umol/L). Assessment with reference to the normal range. 1- Normal (Within the reference range) 2- Abnormal (Outside the reference range). The purpose is to evaluate whether acupuncture has an effect on CREA, which is a safety assessment. | Baseline period(1 week before enrollment), enrollment 12 weeks (Change from Baseline at 12weeks) | |
Other | Prothrombin time (PT) | Normal value (unit): 11-14(s). Assessment with reference to the normal range. 1- Normal (Within the reference range) 2- Abnormal (Outside the reference range). The purpose is to evaluate whether acupuncture has an effect on PT, which is a safety assessment. | Baseline period(1 week before enrollment), enrollment 12 weeks (Change from Baseline at 12weeks) | |
Other | Activated partial thromboplastin time(ATPP) | 10.Activated partial thromboplastin time(ATPP) | Baseline period(1 week before enrollment), enrollment 12 weeks (Change from Baseline at 12weeks) | |
Other | Thrombin time(TT) | Normal value (unit): 20 (s). Assessment with reference to the normal range. 1- Normal (Within the reference range) 2- Abnormal (Outside the reference range). The purpose is to evaluate whether acupuncture has an effect on TT, which is a safety assessment. | Baseline period(1 week before enrollment), enrollment 12 weeks (Change from Baseline at 12weeks) | |
Other | Fibrinogen concentration(FBI) | Assessment with reference to the normal range. 1- Normal (Within the reference range) 2- Abnormal (Outside the reference range). The purpose is to evaluate whether acupuncture has an effect on FBI, which is a safety assessment. | Baseline period(1 week before enrollment), enrollment 12 weeks (Change from Baseline at 12weeks) | |
Primary | The value of anti-mullerian hormone(AMH) | This index is used to measure ovarian hormone secretion function and assess ovarian reserve function. Instrument:Beckman Coulter Access automatic immune analyzer.
Method: Venous blood will be collected on an empty stomach at 3-5mL in the morning on the 2nd to 4th day of menstruation, centrifuged at 5000r/min for 3min with a high-speed centrifuge, and the upper serum is taken and stored in a -80? refrigerator for test. Unit: ng/mL. |
3 checkpoints. Baseline period(1 week before enrollment), enrollment 12 weeks (Change from Baseline at 12weeks) and enrollment 24 weeks(Changes at 12 weeks of treatment and 12 weeks of follow-up). | |
Secondary | The number of antral follicle count (AFC) | The number of antral follicles was detected by three-dimensional B-ultrasound to evaluate ovarian reserve function. The smaller the number, the more severe the symptoms. | 3 checkpoints. Baseline period(1 week before enrollment), enrollment 12 weeks (Change from Baseline at 12weeks) and enrollment 24 weeks(Changes at 12 weeks of treatment and 12 weeks of follow-up). | |
Secondary | Sex hormone: follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2) | Use Chemiluminescence immunoassay to detected the ovarian hormone secretion. On the 2nd to 4th day of menstruation, 5 ml of venous blood will be collected from subjects in the morning under the condition of fasting for 8 h and put into the test tube without anticoagulant. The collected samples will be centrifuged to separate the upper serum and stored in the refrigerator at -70? for examination. Unit: FSH(mLU/mL), LH(mLU/mL), E2( pg/mL). | 3 checkpoints. Baseline period(1 week before enrollment), enrollment 12 weeks (Change from Baseline at 12weeks) and enrollment 24 weeks(Changes at 12 weeks of treatment and 12 weeks of follow-up). | |
Secondary | The total score of the modified Kupperman scale | To evaluate the severity of perimenopausal syndrome symptoms.Each item has a different basic and severity score, the severity score is calculated according to 0-3 points, and the symptom score is calculated as follows:Symptom score=basic score×severity score.The sum of each symptom score is the total score; the higher the total score, the more severe the disease. Patients with a normal score have <6 points. This with mild, moderate, and severe disease have 6-15, 16-30, and >30 points, respectively. | 3 checkpoints. Baseline period(1 week before enrollment), enrollment 12 weeks (Change from Baseline at 12weeks) and enrollment 24 weeks(Changes at 12 weeks of treatment and 12 weeks of follow-up). | |
Secondary | The total score of the perimenopausal quality of life score | 29 items, divided into Vasomotor symptoms, psychosocial function, physical function, sexual life, each item scored 1-7 points.
Dimension score = Total score of item questions in each dimension ? Number of questions Total score = Sum of four dimensions scores |
3 checkpoints. Baseline period(1 week before enrollment), enrollment 12 weeks (Change from Baseline at 12weeks) and enrollment 24 weeks(Changes at 12 weeks of treatment and 12 weeks of follow-up). | |
Secondary | The total score of the self-rating anxiety Scale | Reflect anxiety level. 20 items, each item 1-4 points, total score and standard score (total score ×1.25), the higher the score, the more severe symptoms. | 3 checkpoints. Baseline period(1 week before enrollment), enrollment 12 weeks (Change from Baseline at 12weeks) and enrollment 24 weeks(Changes at 12 weeks of treatment and 12 weeks of follow-up). | |
Secondary | The total score of the self-rating Depression Scale | Reflect the degree of depression. 20 items, each item 1-4 points, total score and standard score (total score ×1.25), the higher the score, the more severe symptoms. | 3 checkpoints. Baseline period(1 week before enrollment), enrollment 12 weeks (Change from Baseline at 12weeks) and enrollment 24 weeks(Changes at 12 weeks of treatment and 12 weeks of follow-up). | |
Secondary | Menstrual assessment | Menstruation was assessed throughout the study period (a total of 7 times). The evaluation consists of 6 items, each item is scored (0 to 4 points), and the total score is calculated for each time. The lower the total score is, the better the menstrual condition is. | Enrollment 1?4?8?12?16?20?24 weeks. |
Status | Clinical Trial | Phase | |
---|---|---|---|
Recruiting |
NCT02896023 -
Predictors of Ovarian Reserve in Infertile Women
|
Phase 4 | |
Recruiting |
NCT02240342 -
Bone Marrow Transplantation to Promote Follicle Recruitment in Poor Ovarian Reserve
|
N/A | |
Completed |
NCT01268930 -
Comparison of Hemostatic Matrix and Bipolar Coagulation in Surgical Treatment of Endometriomas
|
N/A | |
Completed |
NCT03492632 -
Ovarian Reserve in Patients With Psoriasis
|
||
Withdrawn |
NCT02728245 -
Trial Comparing Preoperative Dienogest Therapy Followed by Surgery vs. Upfront Surgery to Save Ovarian Reserve in Young Women With Ovarian Endometrioma
|
Phase 2 | |
Completed |
NCT05567731 -
Effect of GnRH Agonist Treatment Protocols on Ovarian Reserve
|
N/A | |
Completed |
NCT03989921 -
Ovarian Reserve and Embryo Implantation
|
||
Recruiting |
NCT02995343 -
Evaluation of the Ovarian Reserve in Patients Who Hypogastric Arteries and or Uterine Arteries Had Been Ligated
|
N/A | |
Withdrawn |
NCT01966536 -
CD133 Transplantation to Generate Oocytes in Poor Ovarian Reserve
|
N/A | |
Recruiting |
NCT04151173 -
Assessment of Ovarian Reserve After Laparoscopic Cystectomy Versus Aspiration/Electrocoagulation in the Treatment of Ovarian Endometrioma
|
N/A | |
Enrolling by invitation |
NCT02377128 -
Ovarian Reserve Following Bilateral Salpingectomy Versus Tubal Ligation During Cesarean Section
|
N/A | |
Recruiting |
NCT03620838 -
Endometrioma Per se Versus Treatment Related Reduction in Ovarian Reserve (ERROR-2 Trial)
|
||
Completed |
NCT02009228 -
Single-port LC Might be Preferable for Managing Ovarian Dermoid Cyst.
|
Phase 3 | |
Completed |
NCT04762615 -
Impact of Underlying Renal Disease and Immunosuppressive Regimen on Ovarian Reserve in Renal Patients
|
||
Recruiting |
NCT03009370 -
The Effect of the Ovarian Reserve on the Recurrent Pregnancy Loss
|
N/A | |
Completed |
NCT03621683 -
Antioxidants vs. Ovarian Bio-stimulation Therapy to Rescue the Ovarian Reserve
|
N/A | |
Completed |
NCT04058678 -
Effects of Telomerase Reactivation With Danazol in Ovarian Function.
|
N/A | |
Recruiting |
NCT04487925 -
Controlled Ovarian Stimulation Versus Modified Natural Cycles in Poor Responders
|
Phase 4 | |
Terminated |
NCT01114464 -
Impact of Chemotherapy on Ovarian Reserve in Young Women With Breast Cancer
|
N/A | |
Active, not recruiting |
NCT00928044 -
The Impact of Gynecological Surgery on Ovarian Function in Women of Reproductive Age: Postoperative Changes of Serum Anti-Müllerian Hormone (AMH)
|
N/A |