Clinical Trials Logo

Clinical Trial Summary

Nerves are the rate limiting factor that control muscle function. However, it is unknown if a nutritional supplement can change the nerve-muscle interaction, and therefore alter human performance. This study will be the first to examine the effects of omega-3 supplementation on neuromuscular function in trained individuals.


Clinical Trial Description

Participants will come to the lab for 3 sessions, one for familiarization and two for testing.

Familiarization - At this time, anthropometric measurements including height, weight, BMI (body mass index) and body composition will be determined. Participants will be familiarized with the interpolated twitch technique and performing a maximal voluntary contraction of the quadriceps muscle. Next, Participant's 10 repetition maximum squat weight will be established by starting at a participant selected weight and gradually increasing until only 10 squat repetitions can be completed. A VO2max (maximum oxygen consumption) test will be performed on a cycle ergometer using standard methods.

On testing sessions 1 and 2 the following neuromuscular and performance measures will be evaluated:

1. Quadriceps muscle voluntary activation will be assessed using the interpolated twitch technique and doublet twitch technique. Participants will be seated and instructed to perform three 5 second maximal voluntary isometric contractions at least 1 minute apart - participant's ankle will be restrained by a padded cuff attached to a load cell.

For the second maximal isometric contraction at 3 seconds into the contraction, a square wave superimposed stimulus will be delivered for 1 milliseconds to the femoral nerve from a monopolar touch-proof cathode electrode secured in the femoral triangle. 2, 7 and 12 seconds after the contraction a resting twitch will be delivered for comparison of muscle force.

For the third maximal isometric contraction, at 3 seconds into the contraction, two 1 millisecond square wave superimposed stimuli at 100 Hz will be delivered 10 milliseconds apart. 2 seconds after the contraction the same stimulus will be applied. At 7 seconds after, two stimuli will be delivered 100 milliseconds at 10 Hz apart and 12 seconds after a single stimulus will be delivered.

The percutaneous electrical stimulation will be delivered from a Biopac stimulator (BSLSTMA, Biopac Systems, Santa Barbara, California) with a maximum voltage of 400 V. During familiarization, participants will receive several submaximal stimulations for acclimatization before the current intensity is gradually increased until peak twitch torque and muscle action potential amplitude is reached. A stimulus of 1.2 times the current required to elicit peak twitch torque and action potential amplitude will be used to ensure maximal activation. The interpolated twitch technique will be used to examine the difference in muscle activation during maximal contraction and a superimposed stimulation. Voluntary activation will be calculated using the formula:

Voluntary activation (%) = (1- superimposed twitch / twitch evoked at rest) x 100

Surface self-adhesive silver chloride electrodes will be placed over the belly of the vastus lateralis, vastus medialis and biceps femoris muscles to measure muscle electrical activity (electromyography) during maximal voluntary contractions as previously described.

2. Vertical jump - Quadriceps power will be assessed using the vertical jump test on a force plate (AMTI, Watertown, Massachusetts, USA). Participants will complete two attempts to jump as high as possible from crouched and two attempts using the counter jump technique (participants can start from standing, bend the knees and then jump).

3. Push-up - Upper body strength endurance will be examined by having the participant complete as many push-ups as possible in one-minute. Participants will be given one-minute rest before being asked to complete as many push-ups as possible in a minute. A piece of soft foam will be attached to the floor to provide participants feedback as to how low the must go when performing each push-up.

4. 10RM (10 repetition maximum) squat - After completing an optional 2 to 4 repetitions of 75% of the their 10RM weight as determined during baseline data collection, participants will complete as many repetitions as possible.

5. Wingate test - Participants will be fitted to a cycle ergometer (Excalibur Sport V2.0, Lode, Groningen, The Netherlands). They will then cycle against a fixed resistance of 0.075kg / kg body mass for 30 seconds using their maximum effort. Verbal encouragement will be provided to the participant to help produce their maximum effort.

6. Cycling time trial - Participants will complete a 10km time trial on a cycle ergometer (Excalibur Sport V2.0, Lode, Groningen, The Netherlands). Participants' inspired and expired gasses will be measured using a metabolic cart (Cortex, Leipzig, Germany). Gas variables and cycling power output will be measured using the supplied software. This change in protocol does not alter the risk to participants as their level of conditioning is quite high and similar levels of exertion would be required in their daily training.

Supplementation - In between sessions 1 and 2, there is a 21 day break for which participants will be computer randomized to either the omega-3 supplementation or an olive oil placebo. Participants will take a 2.5 mL serving of seal oil (2500 mg n-3, 255 mg DHA (docosahexaenoic acid ) and 187.5 mg EPA (eicosapentaenoic acid) with 500 IU vitamin D3 and 1000 IU vitamin A) (Auum Inc., Timmons, On) or 2.5 mL olive oil (Bertolli, Mississauga, Ont) with 500 IU vitamin D and 1000 IU vitamin A added twice daily with breakfast and dinner.

All supplementation amounts are within the amounts specified by Health Canada Guidelines.

Session 2 - After the 21 day supplementation period, participants will return to the lab for a second evaluatory session using identical protocols to session 1. ;


Study Design

Allocation: Randomized, Intervention Model: Parallel Assignment, Masking: Double Blind (Subject, Investigator)


Related Conditions & MeSH terms


NCT number NCT02014233
Study type Interventional
Source University of Toronto
Contact
Status Completed
Phase N/A
Start date June 2013
Completion date March 2014

See also
  Status Clinical Trial Phase
Recruiting NCT06061367 - Muscles Strength and Gait Parameteres After TKA
Completed NCT04136821 - The Long-term Effects of Oceanix™ on Resistance Training Adaptations N/A
Recruiting NCT05945641 - Effect of Low-load Resistance Training vs. High-intensity Interval Training on Local Muscle Endurance N/A
Not yet recruiting NCT05510648 - Evaluation of the Effect of High-intensity Laser Therapy in Knee Osteoarthritis N/A
Completed NCT03715920 - Comparison of Different Quadriceps Femoris Isometric Strengthening Methods N/A
Terminated NCT01561404 - Effect of the Inhibition of the Mammalian Target of Rapamycin on Metabolism and Exercise Phase 4
Completed NCT04535336 - Vitality Acupunch Exercise Program for Older Adults With Sarcopenia N/A
Completed NCT04880486 - Weight Training With VR in Out-Patients With Acute Exacerbation of Chronic Obstructive Pulmonary Disease N/A
Recruiting NCT06061315 - Effect of Collagen Peptides, in Combination With Resistance Training, on Body Composition and Muscle Strength in Untrained Men N/A
Completed NCT05366348 - The Effects of Horse-back Riding on Core Muscles in Adolescents N/A
Completed NCT04213586 - Effects of Whey Protein and Collagen Supplementation N/A
Completed NCT04626817 - Effect of Oral Isotretinoin on Muscle Strength in Patients With Acne Vulgaris: A Prospective Controlled Study N/A
Completed NCT03922113 - Muscle Function After Intensive Care
Completed NCT01449097 - Adductor-Canal-Blockade Versus the Femoral Nerve Block Effect on Muscle Strength and Mobilization in Healthy Volunteers Phase 4
Completed NCT06196268 - Core Strengthening vs Pilates Exercises on Posture, Body Awareness and Fatigue Among Female Athletes N/A
Completed NCT05882981 - Increased Femoral Anteversion and Physical Performance
Completed NCT00059436 - Mental Effort and Muscle Strength Phase 1
Recruiting NCT06410014 - Assessment of Overall Functioning in Patients With Complex Health Issues
Completed NCT04239417 - Effect of Preoperative Abdominal Exercises and Russian Current on Muscle Strength Post Ventral Hernioplasty N/A
Completed NCT04315077 - The Short Term Effects of Oceanix Supplementation on Recovery N/A