Interleukin-6 Secretion in Adipose Tissue Following Sprint Exercise

Metabolism in Adipose Tissue Clinical Trial

Official title:

Metabolism in Adipose Tissue Followed Following Sprint Exercise

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT04566354
• Other study ID #: 2015/1054-32
• Status: Completed
• Phase: N/A
• Start date: September 2015
• Est. completion date: September 2017

Study information

• Verified date: September 2020
• Source: Karolinska Institutet
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

Low volume sprint exercise training has been shown to reduce body fat despite small total energy expenditure (for ref see Gillen and Gibala 2014). IL-6 has been shown to increase adipose tissue lipolysis in vitro. Therefore, in an initial study on effects of sprint exercise on adipose tissue metabolism the investigators will examine acute short-term exercise effects on adipose tissue IL-6 exchange.

Description:

PURPOSE: Low volume sprint exercise training has been shown to reduce body fat despite small total energy expenditure (for ref see Gillen and Gibala 2014). IL-6 has been shown to increase adipose tissue lipolysis in vitro. Therefore, in an initial study on effects of sprint exercise on adipose tissue metabolism the investigators examined acute short-term effects on adipose tissue IL-6 exchange.

METHODS: Four female and four male subjects perform repeated sprint exercise (3 x 30s with 20 min rest between; Wingate-test). Blood samples are repeatedly obtained, up to 120 min after the last sprint (9 min post ex), from catheters inserted percutaneously into brachial artery and a superficial subcutaneous vein on the anterior abdominal wall providing access to the venous drainage from the subcutaneous adipose tissue and analyzed for IL-6 by ELISA and lactate by spectrophotometry. Fat biopsies from the stomach will be obtained before, 15 min and 120 min after the last sprint. Blood flow will be determined by administration of Xenon-133 in the adipose tissue of the stomach. The release rate of Xenon-133 will be registrated continuously during the total investigation.

SUPPORT: This study is supported by grants from the Swedish National Center for Research in Sports

Recruitment information / eligibility

• Status: Completed
• Enrollment: 18
• Est. completion date: September 2017
• Est. primary completion date: September 2016
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 20 Years to 40 Years

Eligibility

Inclusion Criteria:

• Physical active

• Healthy

Exclusion Criteria:

• Smokers

• Pregnant

• On medication

• No infection

• No asthma

Related Conditions & MeSH terms

• Metabolism in Adipose Tissue

Intervention

Other:
• Repeated 30-s sprint exercise
Analyze of Interleukin-6 concentration before and after repeated 30-s sprint exercise

Locations

Country	Name	City	State
Sweden	Karolinska Institutet/Karolinska University Hospital	Stockholm

Sponsors (1)

Lead Sponsor	Collaborator
Karolinska Institutet

Country where clinical trial is conducted

Sweden, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Il-6 secretion in adipose tissue before and after 30-s repeated sprint exercise	Stomach fat biopsies and arterial and stomach vein blood are obtained	Before 30-s repeated sprint exercise
Primary	Il-6 secretion in adipose tissue before and after 30-s repeated sprint exercise	Stomach fat biopsies and arterial and stomach vein blood are obtained	After 30-s repeated sprint exercise
Secondary	Changes in blood flow in adipose tissue	Xenon-133 is administered by a subcutaneous injection into the abdominal adipose tissue of approximately 1-2 MBq of Xenon-133 in the form of gas, at rest before work.	Before 30-s repeated sprint exercise
Secondary	Changes in blood flow in adipose tissue	The rinsing rate of Xenon-133 from the tissue depot is continuously recorded during the study period by a non-invasive method using a portable scintillation detector system (Mediscint) up to 60 minutes after the last sprint work.	After 30-s repeated sprint exercise
Secondary	Changes in different metabolites	Analysed for lactate, ammonia and IL-6	Before 30-s repeated sprint exercise
Secondary	Changes in different metabolites	Analysed for lactate, ammonia and IL-6	After 30-s repeated sprint exercise