Clinical Trial Details
— Status: Enrolling by invitation
Administrative data
| NCT number |
NCT06399367 |
| Other study ID # |
16/10/19 |
| Secondary ID |
|
| Status |
Enrolling by invitation |
| Phase |
|
| First received |
|
| Last updated |
|
| Start date |
August 2024 |
| Est. completion date |
December 1, 2028 |
Study information
| Verified date |
May 2024 |
| Source |
University Hospital Goettingen |
| Contact |
n/a |
| Is FDA regulated |
No |
| Health authority |
|
| Study type |
Observational
|
Clinical Trial Summary
This study aims to analyze the fatty tissue architecture of the subcutaneous tissue in
patients from the plastic surgery department.
Plastic surgery patients show a wide variety of subcutaneous fatty tissue structures during
clinical examination. These include patients with edema of the extremities such as lipedema
or lymphedema.
Fatty tissue architecture plays a major role in our everyday lives, as wound healing and scar
formation, for example, are influenced by the blood flow to the overlying skin. The fatty
tissue architecture, especially in the subcutaneous fatty tissue, also plays a major role in
our appearance. An analysis of the architecture can potentially provide information about the
genesis of different skin fold formations. The aim of this study is to quantitatively
describe structural differences in adipose tissue architecture.
Adipose tissue architecture is still a largely unexplored area because imaging has not been
possible to date. MSOT imaging is similar to conventional sonography in that a transducer is
placed on the skin and energy is supplied to the tissue by pulsed laser light instead of
sound. On a macroscopic level, this leads to a constant change of minimal oscillations of
individual tissue components. The resulting sound waves can then be detected by the same
transducer. Previous studies have shown that the quantitative determination of hemoglobin can
be used to obtain information on blood circulation and inflammatory activity. In the extended
spectrum, in contrast, not only hemoglobin and its oxygenation stages but also other
biomarkers such as collagens and lipids can be detected. This is very useful for imaging of
fat, lymphatics and normal and abnormal blood vessels in vascular malformations.
This process was largely researched by the working group of Prof. Ntziachristos (Helmholtz
Center Munich and Technical University of Munich) and Prof. Razansky (Eidgenösische
Technische Hochschule Zurich) and is being further developed into a clinically applicable
technology and sold commercially by the company iThera. As a first series of demonstrative
clinical studies following rigorous technical development, MSOT will serve as a key tool for
research partners in the investigation of several diseases that remain poorly-understood and
have limited treatment options. These parallel studies will focus on lipedema and lymphedema
as well as vascular malformations - three distinct disease groups with similarly unmet
clinical needs for appropriate imaging modalities and high potential of translation to
further major disease areas. By focusing on two unrelated diseases, this project will show
the wide-reaching application of this innovative imaging approach. Following successful
proof-of-principle validation in a clinical research environment, full exploitation and
dissemination of the results will strive to deliver MSOT to the greater scientific community.
The main objectives are to confirm/validate the spectral profile of fat and vasculature on
MSOT in lipedema patients, to establish the spectral profile of vascular malformations based
on MSOT for adults and children and to establish the spectral profile and imaging of
lymphatic vessels.
With a detailed analysis of the architecture, our understanding of the physiology and
pathology of the skin may be enhanced.
Description:
To date, the diagnostic path of illnesses involving an altered adipose tissue architecture
are usually slow and depend heavily on the experience of the examiner, so it often takes
several years before the patient is properly treated. Current imaging methods for the
quantitative assessment of subcutaneous fat tissue only provide non-specific findings. With
our study, the investigators present a novel imaging method for examining adipose tissue.
Optoacoustics is an innovative hybrid imaging method that combines the high contrast of
optical imaging with the high resolution of acoustics. The MSOT system used consists of a
laser in the visible near-infrared and far-infrared wavelength range (660nm -1300nm) and an
ultrasonic sensor connected to a computer. Using optical components, the area to be measured
is illuminated with ultra-short light pulses of selectable wavelength. The light is absorbed
to varying degrees inside the object - depending on the properties of the tissue. The Laser
light stimulates chromophores such as hemoglobin or melanin in the human body, which results
in an expansion and subsequent contraction of the tissue, the so-called thermoelastic effect.
The local microscopic volume fluctuations emit pressure waves in the ultrasonic range
(photoacoustic effect). The skin surface is scanned using high-precision microstages. At each
location, the ultrasound signals generated by the light pulse are recorded tomographically
outside the object using the detector and stored on the computer. With the help of special
algorithms, the properties of the tissue can be reconstructed on the computer from the
measured ultrasound signal. MSOT enables non-invasive, non-ionizing imaging with high spatial
resolution (80 μm), which enables specific visualization of endogenous tissue pigments such
as collagen fibers, fat, melanin, oxyhemoglobin and deoxyhemoglobin. Lasers for stimulating
certain chromophores are already in wide therapeutic use in dermatology; their effects and
side effects are known and are minimal to non-existent when a suitable energy is selected.
The penetration depth of MSOT is significantly greater compared to other optical imaging
methods.
In addition, this non-invasive, real-time measuring system can be used in numerous everyday
clinical questions that otherwise require more invasive methods like CT or MRI. Apart from
the examination of fatty tissue architecture, MSOT could be used pre- and perioperatively to
visualize vessels, for example to optimize or even primarily enable the perfusion of flaps.
The aim is to display and analyze the architecture of the subcutaneous fatty tissue in
plastic surgery patients using MSOT.
The study is designed as a prospective cohort study of subjects recruited from the plastic
surgery patient population. MSOT measurements are carried out on 50 subjects with clinically
healthy subcutaneous fatty tissue and 50 subjects with changes in the subcutaneous fatty
tissue (e.g. lipedema). The recorded data is quantified and compared.
The test subjects for this study are recruited from the trauma surgery, orthopedics and
plastic surgery clinic and polyclinic. In particular, patients are recruited from the special
plastic surgery consultation hours.
Everyone present wears laser safety goggles. An ultrasound gel film is applied to
approximately 1 cm² of skin in several places on the selected extremities of the patient and
the detector is placed on the gel film. These areas of skin are then scanned, with the areas
to be examined being illuminated with ultra-short light pulses of a selectable wavelength.
The resulting sound waves are detected with ultrasonic sensors and reconstructed on the
computer and spectrally separated. A single measurement takes approximately 5 minutes.
Including preparation, measuring the three skin areas will take approximately 20 minutes.
Invasive procedures are not planned in this project.
The scanned areas are exported from the software as image material and stored pseudonymously.
There is also photo documentation of the soft tissue that was scanned. These photos are also
saved. To document health data, a lipedema documentation form is completed.
The optoacoustic modality does not require ionizing radiation and is based solely on the
detection of acoustic waves induced by laser light. Both the technology of irradiation with
various lasers and sonography are already widely used in the diagnosis and treatment of skin
diseases. Therefore, their side effects are very predictable and, with appropriately selected
energies, minimal to non-existent. To excite optoacoustic signals, class 4 pulsed lasers in
the visible and near-infrared range (660 - 1300 nm) with a very short pulse duration (9ns)
are used. The American National Standards Institute (ANSI) has set limit values for the use
of lasers of this type, which are not exceeded in the experiments. Theoretically, treatment
errors, such as choosing too high an energy level, can lead to tissue damage depending on the
laser used. Lasers in the area of the chromophore hemoglobin can cause thermal vascular
damage to the smallest and smallest vessels. If the energies are chosen to be very high, the
maximum result could be local damage to the epidermis with subsequent local scarring, similar
to a burn.
Since these effects are known through years of experience in widespread use, none of these
side effects are expected during this study.
The evaluation of the sound waves generated using sonography is considered safe and no side
effects are to be expected.
The laser light is directed onto the skin via the detector. Laser light can therefore only
penetrate to the outside indirectly through scattering and theoretically reach the eyes of
surrounding people. Since laser light with the above specification can be harmful to the
eyes, care is taken to ensure that everyone present wears laser safety glasses during the
measurement. There is virtually no risk of eye injury when wearing laser safety glasses.
The individual discontinuation criteria include intolerance and reactions such as redness.
Previously described undesirable effects during treatment include redness and thermal
vascular damage to soft tissue or subjectively perceived side effects. Another individual
termination criterion is the revocation of the test subjects' consent to participate in the
study or the refusal to wear laser safety glasses.
Termination criteria for the overall study include: unrecoverable defect in the monitoring
system (MSOT), loss of all data. Redness with blistering in the sense of skin burns grade IIa
(not yet described).
The aim of this pilot project is to describe the microarchitecture of the subcutaneous fatty
tissue in patients with and without skin changes (e.g. lipedema) using MSOT. The ability to
visualize these changes could lead to a better understanding of the physiology and
pathophysiology of the skin.
When carrying out the investigation, the following guidelines and laws are observed:
- Declaration of Helsinki (2013) as supplemented by Venice 1983 and Hong Kong 1989
- The vote of the local ethics committee is obtained from the head of the scientific
investigation
- The start and implementation of the investigation are tied to a vote by the ethics
committee. After the investigation has been completed, a report or publication is
submitted to the ethics committee
- The subjects' consent is given voluntarily after the risks have been explained. The test
subjects do not receive any compensation
- Consent can be withdrawn at any time without giving reasons
- If the participant withdraws from the study, data material that has already been
obtained will be destroyed or the subject will be asked whether he or she agrees to the
evaluation of the material
- The names of the test subjects and all other confidential information are subject to
medical confidentiality and the provisions of the Federal Data Protection Act (BDSG) - -
Subject data may only be passed on in pseudonymized form. Third parties do not have
access to the original documents
- Before the start of the study, the test subjects are informed in writing and orally
about the nature and scope of the planned examinations, in particular about the possible
benefits for their health and any risks. The participants consent is documented by
signing the consent form
