Per-protocol Repeat Kidney Biopsy in Incident Cases of Lupus Nephritis

Lupus Nephritis Clinical Trial

— REBIOLUP  

Official title:

Per-protocol Repeat Kidney Biopsy in Incident Cases of Lupus Nephritis

Clinical Trial Details — Status: Recruiting

Administrative data

• NCT number: NCT04449991
• Other study ID #: REBIOLUP
• Status: Recruiting
• Phase: N/A
• Start date: January 5, 2022
• Est. completion date: December 2028

Study information

• Verified date: October 2023
• Source: Karolinska Institutet
• Contact: Ioannis Parodis, MD PhD | Associate Professor
• Phone: +46722321322
• Email: ioannis.parodis[@]ki.se
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

Lupus nephritis (LN) is one of the most severe complications of systemic lupus erythematosus (SLE). Among people living with SLE, 35-60% will develop LN during the course of the disease. This complication is one of the factors that contribute to deterioration of the renal function.

Some centres perform kidney biopsies after completion of treatment for an episode of LN as a part of the treatment evaluation. The term "repeat biopsy" is often used to describe these biopsies. Several studies have reported that repeat kidney biopsies show activity at the level of tissue, even in patients with normal routine blood and urine markers. The investigators strongly believe that this information is important, and should be taken into consideration during decision of treatment. To provide evidence for this, the investigators have designed a collaborative project within the frame of the Lupus Nephritis Trials Network. With this research project, the investigators want to contribute to an increased proportion of patients with LN who achieve remission (inactivity) of LN, and a reduced proportion of patients who worsen in renal function in the long term.

Patients with SLE who develop a first episode of LN will be asked to participate in this project, and will receive treatment according to current guidelines. Half of the patients will undergo a repeat biopsy 12 months later, and half of the patients will not. The selection of patients who will undergo or not undergo repeat biopsy will be random. Patients with high disease activity at the level of kidney tissue will receive more intense immunosuppressive treatment. Patients who have not undergone repeat biopsy will continue to be treated according to standard routine.

The investigators will compare the results of treatment between the group of patients who underwent and the group of patients who did not undergo repeat biopsy, with regard to (i) complete disease inactivity at month 24 and (ii) renal function at month 60 from treatment initiation.

The investigators expect that significantly greater proportions of patients in the repeat biopsy group will have inactive disease at month 24 and adequate levels of renal function at month 60. This will provide support for performing repeat biopsies as a part of the treatment evaluation, in order to optimise the therapeutic management and improve the long-term prognosis of patients with LN.

Description:

Study rationale

Lupus nephritis (LN) affects 35-60% of patients with systemic lupus erythematosus (SLE), and constitutes one of the most severe disease manifestations. Several factors contribute to renal function impairment in LN, including genetic cargo, nephron endowment, disease activity, drug-induced toxicity and renal flares. Five to 20% of the afflicted patients develop end-stage kidney disease (ESKD) within ten years from the first LN episode. Available therapies induce complete renal remission in only 20-30% of patients after six months of treatment, and even patients who respond to therapy may relapse in 35% of the cases.

To date, clinical and laboratory tests cannot reliably reflect histopathological findings, and kidney biopsy is indispensable for diagnosis, classification, and exclusion of mimickers. The role of control biopsies after induction therapy is debatable, despite accumulating evidence of discrepancies between clinical and histological responses. In the concrete, several elegant post-treatment biopsy studies have demonstrated persistent activity at the level of tissue despite adequate clinical response, suggesting that intensified immunosuppression may be required in these patients. However, studies investigating the role of repeat kidney biopsies in treatment evaluation and long-term renal outcome have to date included limited numbers of patients, and varying definitions regarding treatment response and renal outcome. Thus, the concept of a prospective multicentric per-protocol repeat biopsy study to generate evidence for future recommendations for the diagnostic and therapeutic management of LN gains increasing support.

Several studies have sought to identify predictors of long-term renal prognosis, including routine clinical and serological markers. Proteinuria levels < 0.7-0.8 g/day at month 12 after initiation of treatment have been validated in different cohorts as a readily available predictor of favourable long-term renal outcome. However, while the positive predictive value (PPV) of this target was high, the negative predictive value (NPV) was poor in two of three studies, since most patients not achieving the proteinuria target still had a good long-term outcome (Tamirou et al. 2015; Ugolini-Lopes et al. 2017). Associations between chronic tissue damage in repeat biopsies and long-term renal function impairment have been demonstrated in European and Hispanic LN populations (Piñeiro et al. 2016). It was recently demonstrated in a retrospective study that a high grade of residual activity in per-protocol repeat biopsies predicted subsequent renal relapse in patients with incident LN, and a high grade of chronic damage in repeat biopsies predicted long-term renal function impairment. More specifically, NIH activity index scores > 3 predicted subsequent renal flares, whereas NIH chronicity index scores > 3 were associated with renal function deterioration in the long term. It is worth noting that active lesions in glomeruli mostly accounted for the former association with relapses, whereas chronic damage in the tubulointerstitial compartment were found to be a more important contributor to the latter association with long-term renal function (Parodis et al. 2020).

Study objectives

The objectives of the project will be

• to determine the percentage of LN patients in histopathological remission after 12 months of standard of care immunosuppression;

• to correlate histological and immunological (immune deposits) response to therapy with clinical response;

• to evaluate whether therapeutic decisions steered by the results of a per-protocol repeat kidney biopsy improve renal outcomes compared with a matched control group of patients who did not undergo repeat kidney biopsy.

• to generate data on how to evaluate response to therapy in pure membranous LN (ISN/RPS class V), as well as the value of the information retrieved from repeat kidney biopsies in portending long-term renal prognosis in this LN subset.

Study design

Patients with an incident biopsy-proven proliferative or membranous LN, or combinations thereof, selected to be initiated at standard of care immunosuppressive therapy with either MMF or EURO-Lupus IV CYC (combined with GCs and ACE inhibitors/ARBs) will be enrolled in this prospective study. Add-on therapies on the top of the aforementioned regimens will be allowed. At baseline, patients will be randomised 1:1 to either undergo or not undergo a per-protocol repeat kidney biopsy at month 12 from baseline. After randomisation, the patients will be followed for one year within the frame of REBIOLUP Part I, which is observational. Inclusion of patients participating in other investigator-initiated or pharmaceutical industry-driven therapeutic trials will be made possible, provided that those trials do not last more than 12 months. At month 12, REBIOLUP patients will enter into Part II, which is interventional and an interval of ± 1 month is allowed.

In patients with 2003 ISN/RPS class III/IV (± V) at baseline and an NIH activity index score > 3 (cut-off based on a recent proof-of-concept retrospective analysis) in the repeat kidney biopsy, the immunosuppressive therapy will be intensified based on the physician's and patient's shared decision (see recommendations below). In cases of pure membranous nephritis (ISN/RPS class V) at baseline which transformed to ISN/RPS class III/IV (± V) in the repeat biopsy, the same algorithm will be applied.

In patients with pure membranous (2003 ISN/RPS class V) LN in the repeat biopsy, individual assessment of the biopsy should steer the decision of treatment.

Patients who have not undergone a repeat biopsy will be treated according to standard clinical parameters. Percentages of complete renal response at month 24 and renal impairment at month 60 will be compared between the two study arms.

Patients randomised to the control arm (i.e. to not undergo per-protocol repeat biopsy) who underwent a repeat kidney biopsy upon clinical indication approximately at month 12 from baseline, e.g. due to primary non-response or worsening, will be analysed in the control arm (as per intention) for the primary analysis, and, additionally, together with the patients in the interventional arm in a secondary analysis.

Statistical power calculation and number of subjects needed

The anticipated frequency of CRR at month 24 in patients of the "no repeat biopsy" arm is 40%. The investigators consider that a difference of 20% in favour of the "repeat biopsy" arm would be clinically meaningful (i.e. a frequency of 60% CRR at month 24). To detect such a difference, with an α level of 0.05 and a power of 0.80, a number of 98 patients per arm is required. In order to take an anticipated 5% drop-out proportion into account, 206 patients with incident proliferative [2003 ISN/RPS class III/IV (A or A/C)] lupus nephritis will need to be enrolled. Patients with incident active membranous LN (2003 ISN/RPS class V) will also be enrolled in REBIOLUP; however, the target number of patients per arm will be based on proliferative cases only, since power calculation has been based on data from studies of proliferative LN.

Based on an anticipated yearly inclusion rate of 3-5 patients per year per centre, i.e. 6-10 patients during a 2-year period, approximately 20-30 centres of LN expertise will need to participate in the study.

Recruitment information / eligibility

• Status: Recruiting
• Enrollment: 206
• Est. completion date: December 2028
• Est. primary completion date: December 2028
• Accepts healthy volunteers: No
• Gender: All
• Age group: 18 Years and older

Eligibility

Inclusion Criteria:

• Fulfilment of the EULAR/ACR classification criteria of SLE.

• 18 years of age or above.

• Incident biopsy-proven proliferative or membranous LN, or combinations thereof (with UPCR = 1 g/g), i.e. 2003 ISN/RPS class III (A or A/C) ± V, class IV (A or A/C) ± V, or class V.

• Consent to the possibility of a repeat kidney biopsy at month 12 from baseline.

• Initiation of the following treatment regimens:

• intravenous pulses of methylprednisolone (total dose of 500-3000 mg);

• oral prednisone or equivalent 0.3-0.5 mg/kg/day with tapering;

• hydroxychloroquine unless contraindicated;

• angiotensin-converting enzyme (ACE) inhibitors or angiotensin II receptor blockers (ARBs);

• either one of mycophenolate mofetil (MMF) equivalent dose 2-3 g/day, or IV cyclophosphamide (CYC) according to the Euro-Lupus regimen;

• the NIH protocol for IV CYC (0.5-0.75 g/m2 monthly for six months) could be considered in severe cases;

• add-on therapies (e.g. calcineurin inhibitors, biologics) to the above two regimens are optional.

Exclusion Criteria:

• Antiphospholipid syndrome nephropathy (APSN).

• Pregnancy at baseline (pregnancy during follow-up will not lead to exclusion).

• Medical contraindications to kidney biopsy, e.g. thrombocytopenia < 50,000/µL, uncontrolled hypertension or end-stage kidney disease (ESKD).

• Anticipated non-adherence to therapy.

• Medical conditions interfering with outcome evaluations.

• Inability to read and/or sign the informed consent form.

Related Conditions & MeSH terms

• Lupus Nephritis
• Nephritis

Intervention

Procedure:
• Repeat kidney biopsy
Intensification of immunosuppression if NIH AI > 3

Locations

Country	Name	City	State
Sweden	Karolinska University Hospital	Stockholm

Sponsors (2)

Lead Sponsor	Collaborator
Karolinska Institutet	Université Catholique de Louvain

Country where clinical trial is conducted

Sweden, 

References & Publications (4)

Parodis I, Adamichou C, Aydin S, Gomez A, Demoulin N, Weinmann-Menke J, Houssiau FA, Tamirou F. Per-protocol repeat kidney biopsy portends relapse and long-term outcome in incident cases of proliferative lupus nephritis. Rheumatology (Oxford). 2020 Nov 1;59(11):3424-3434. doi: 10.1093/rheumatology/keaa129. — View Citation

Pineiro GJ, Arrizabalaga P, Sole M, Abellana RM, Espinosa G, Cervera R. Repeated Renal Biopsy - A Predictive Tool to Assess the Probability of Renal Flare in Lupus Nephritis. Am J Nephrol. 2016;44(6):439-446. doi: 10.1159/000452229. Epub 2016 Oct 28. — View Citation

Tamirou F, Lauwerys BR, Dall'Era M, Mackay M, Rovin B, Cervera R, Houssiau FA; MAINTAIN Nephritis Trial Investigators. A proteinuria cut-off level of 0.7 g/day after 12 months of treatment best predicts long-term renal outcome in lupus nephritis: data from the MAINTAIN Nephritis Trial. Lupus Sci Med. 2015 Nov 12;2(1):e000123. doi: 10.1136/lupus-2015-000123. eCollection 2015. — View Citation

Ugolini-Lopes MR, Seguro LPC, Castro MXF, Daffre D, Lopes AC, Borba EF, Bonfa E. Early proteinuria response: a valid real-life situation predictor of long-term lupus renal outcome in an ethnically diverse group with severe biopsy-proven nephritis? Lupus Sci Med. 2017 Jun 12;4(1):e000213. doi: 10.1136/lupus-2017-000213. eCollection 2017. — View Citation

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Complete Renal Response (CRR) at month 24 from baseline	UPCR = 0.5 g/g in two consecutive first-morning void urine specimens and no increase in serum creatinine by = 25% from baseline.	Month 24 from baseline
Secondary	Renal function impairment at month 60	Sustained (i.e. observed in at least two consecutive measurements on different dates) increase in serum creatinine by = 25% from baseline.	Month 60 from baseline
Secondary	Renal relapse (proteinuric flare)	Reappearance of UPCR > 1.0 g/g from month 12 onwards, leading to intensification or change of immunosuppressive therapy. This increased proteinuria has to be sustained (i.e. observed in at least two consecutive measurements on different dates), occurring after an initial response to induction therapy (defined as sustained UPCR < 0.5 g/g).	Between month 12 and month 60 from baseline
Secondary	Reabsorption of immune deposits in repeat kidney biopsies	= 50% decrease in intensity and amount of immune deposits in electron microscopy.	Month 12 from baseline
Secondary	End-stage kidney disease (ESKD)	Chronic kidney disease stage 5 and/or dialysis will be used to assess ESKD.	Between month 12 and month 60 from baseline
Secondary	Mortality rate	The number of deaths during the study period.	Between month 12 and month 60 from baseline