Acute Effect of Fructose on Lipid Metabolism and Gender Differences

Lipid Metabolism Clinical Trial

Official title:

Acute Effect of Fructose on Lipid Metabolism and Gender Differences

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT00620360
• Other study ID #: 279/07/CE/FBM
• Status: Completed
• Phase: N/A
• First received: February 8, 2008
• Last updated: February 23, 2012
• Start date: January 2008
• Est. completion date: December 2008

Study information

• Verified date: February 2012
• Source: University of Lausanne
• Contact: n/a
• Is FDA regulated: No
• Health authority: Switzerland: Ethikkommission
• Study type: Interventional

Clinical Trial Summary

It has been widely documented that fructose overfeeding increases plasma triglycerides and hepatic de novo lipogenesis, and impairs insulin sensitivity in healthy male volunteers. The effect of gender on the metabolic responses to fructose remains an important open question, however.

The objective of this study is to compare the effect of an acute oral fructose load on carbohydrate and lipid metabolism in healthy young males and females.

Description:

The study is aimed at comparing the effects of oral fructose on several specific metabolic pathways in males and females.Participants will receive an isoenergetic diet containing 55% carbohydrate, 15% protein and 35% lipids for three days prior to testing. After this period of controlled diet, they will be studied for 2 hours in the post-absorptive state (Time 0-120 min) and over a 6 hours period (Time 120-480 min) during which they will receive 4 loads of 0,30 g/kg fat free mass U-13C labelled fructose, at times 120, 180, 240, 300. Throughout the study, deuterated glucose and glycerol will be infused to monitor whole body glucose production and glycerol turnover.

The following parameters will be monitored in basal conditions and after the ingestion of the load of fructose:

• Glycerol turnover(glycerol 2H5)

• de novo lipogenesis (incorporation of 13C into palmitate of VLDLs)

• whole body energy expenditure and net substrate oxydation (indirect calorimetry)

• net fructose oxidation (breath 13CO2)

• glucose turnover: (6,6 2H2 Glucose)

• plasma glucose, free fatty acids, ketone bodies, lactate, insulin, triacylglycerol, total cholesterol, VLDL, LDL, and HDL subfractions

An adipose tissue (periumbilical subcutaneous) biopsy will be obtaine by needle aspiration under local anesthesia in fasting conditions (time 0 min) and after fructose (time 480 min) to assess the effects of fructose on adipose gene expression profile. Key genes involved in the regulation of carbohydrate (GLUT 4, hexokinase, PDH-kinase), lipid (FAT-CD36, FABP, acetylCoA carboxylase, malonyl-CoA decarboxylase, PPARg) and energy metabolism (PGC-1a, UCP2)will be monitored

Results obtained in males and females will be compared with two-way analysis of variance

Recruitment information / eligibility

• Status: Completed
• Enrollment: 18
• Est. completion date: December 2008
• Est. primary completion date: December 2008
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Both
• Age group: 18 Years to 35 Years

Eligibility

Inclusion Criteria:

• Males and females

• Age 18-35

• Healthy

• Body mass indexes (BMI) between 19 and 25 kg/m2

• Informed consent obtained

Exclusion Criteria:

• Smokers

• Alcohol intake > 30g/day

• Drug abuse

• Diabetes mellitus

Study Design

Allocation: Non-Randomized, Intervention Model: Single Group Assignment, Masking: Open Label, Primary Purpose: Basic Science

Related Conditions & MeSH terms

• Lipid Metabolism

Intervention

Dietary Supplement:
• fructose
acute administration of 4 times 0.3g fructose/kg lean body mass
• Fructose
acute administration of 4 times 0.3 g/kg fat-free mass oral fructose

Locations

Country	Name	City	State
Switzerland	Centre Hospitalier Universitaire Vaudois	Lausanne	Vaud

Sponsors (1)

Lead Sponsor	Collaborator
University of Lausanne

Country where clinical trial is conducted

Switzerland, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Hepatic de novo lipogenesis		acute effect of dietary fructose (within 6 hours)	No
Secondary	Whole body lipid oxidation, glucose turnover, glycerol turnover, plasma substrates, hormone and energy expenditure (free fatty acid, glucose, lactate, beta-hydroxybutyrate, glycerol, VLDL- Triglycerides and insulin) expression of key adipose genes		acute effect of fructose (within 6 hours)	No