Infant Clinical Trial
Official title:
Fecal Samples for Metabolomic and Microbiomic Studies: Effects of Preparation and Storage
The purpose of this study is to evaluate the effects of storage and handling on fecal samples collected for metabolomic, proteomic and microbiomic and analysis.
Background
Human fecal collection for clinical or research studies usually occurs at the residence of
the subject, an environment not equipped with measures to accommodate proper preparation and
storage of samples for microbiome and metabolome analysis.
Aims
To evaluate the effects of storage and handling on fecal samples collected for metabolomic,
proteomic and microbiomic and analysis.
Methods
Fecal samples will be collected within several minutes of voiding from healthy infants at
the time of diaper change at day-care center.
Samples will be divided into 7 tubes, and subjected to different storage temperatures,
before preforming microbial analysis by DNA sequencing, and metabolome analysis by H1NMR
spectroscoty.
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