Clinical Trial Details
— Status: Completed
Administrative data
| NCT number |
NCT02762682 |
| Other study ID # |
Its12pgi |
| Secondary ID |
|
| Status |
Completed |
| Phase |
N/A
|
| First received |
May 3, 2016 |
| Last updated |
December 27, 2016 |
| Start date |
July 2015 |
| Est. completion date |
December 2016 |
Study information
| Verified date |
December 2016 |
| Source |
Postgraduate Institute of Medical Education and Research |
| Contact |
n/a |
| Is FDA regulated |
No |
| Health authority |
India: Drugs Controller General of India |
| Study type |
Observational
|
Clinical Trial Summary
PRIMARY OBJECTIVES
• To describe the vitamin B12 status in children with infantile tremor syndrome(ITS) and Pre
ITS
SECONDARY OBJECTIVES
- To study the vitamin B12 status in mothers of children with infantile tremor syndrome
(ITS) and Pre ITS
- To study the blood levels of folate in children with infantile tremor syndrome
(includes Pre ITS) and their mothers.
Description:
Materials and methods
STUDY SETTING: Hospital based study (Advanced Pediatrics Centre, PGIMER) STUDY PERIOD: July
2015 to December 2016 STUDY DESIGN: Cross sectional, Observational Enrolment criteria
Inclusion criteria (cases)
1. Either sex, age less than 3 years
2. Clinical diagnosis of Infantile Tremor Syndrome as evidenced by the following features:
[Developmental delay or regression WITH history of exclusive or predominant breast feeding
WITH two or more of the following; skin pigmentation, hair depigmentation, tremors] ITS:1
and 2 plus tremors PreITS:1 and 2 without tremors Exclusion criteria (cases)
1. Parents refusing consent
2. Later confirmation of an alternative diagnosis
3. Patient has received oral or injectable Vitamin B12 in the past 6 months Controls (C)-
Normally developing children
- Inclusion criteria Developmentally normal child not suffering from any acute or
chronic neurological illness
- Exclusion criteria
1.Parent/individual refusing consent 2.Patient has received oral or injectable Vitamin B12
in the past 6 months
PLAN OF THE STUDY All consecutive patients who present in Pediatric Emergency and OPD in
Advanced pediatric Centre with suspected Infantile tremor syndrome will be screened for
inclusion in the study. Those found eligible would be included after an informed consent.
Baseline clinical and demographic profile will be recorded on a proforma..The study aims at
assessment of vitamin B12 status in children with ITS. The various manifestations of the
syndrome, the presentations, the clinical picture, the development profile, various
haematological and biochemical markers would be studied in a detailed manner and recorded on
a proforma (annexure).
The first section of the proforma is targeted at the clinical picture of the disease. The
presenting features will be recorded. The presenting complaints which can be anything
ranging from an episode of upper respiratory tract infection to an apparently healthy child
presenting with delayed milestones. A brief history of the development milestones achieved
under gross motor, fine motor, language and social milestones is important and form a basis
for screening the developmentally delayed children .A feeding history will be taken and is
important as this disease is more frequently seen in children who are
exclusively/predominantly breastfed. A detailed examination of the child would be done and
the findings recorded. Skin and hair changes are very classical of this disease and hence
will be to be studied and recorded in detail. A neurological examination will be done to
study the neurological status of the children and presence of any extrapyramidal symptoms.
Hematological indices will be recorded. Plasma Vitamin B 12, folate, homocysteine levels
will be studied.Urine methylmalonic acid along with TMS/GCMS will also be done as a part of
this project. Other investigations will be done as per case profile. Any other investigation
if done for the case as a part of the treatment or diagnostic evaluation like liver function
tests, renal function tests , EEG, CT, MRI will also be recorded.
The second section of the proforma consists of assessment of health and nutritional status
of the mother. It aims at establishing any direct relation between the maternal health and
development of ITS like picture in their children. Mother's dietary history will be taken to
find out whether the maternal diet is lacking in Vitamin B12 and folate. A targeted
neurological examination will be done to look for neurological manifestations of vitamin B12
deficiency in form of posterior column tract sensation and presence of peripheral
neuropathy. A lab estimation of maternal Vitamin B12,folate and homocysteine levels will
also be done and recorded.
Blood samples: Sampling would be done along with routine sampling as far as possible to
avoid additional pricks to the child. Plasma would be immediately separated and stored and
tested for Vitamin B12 ,homocysteine, methylmalonic acid and folate levels. This blood would
be taken from sample already taken for other tests to be done in the child. Confidentiality
of records would be maintained. The study is purely observational and will not influence the
management of the child. Though as a standard policy all children with ITS would receive
Vitamin B12, folate and other supplements and diet counseling will be done.
Blood TMS-acylcarnitine and propionylcarnitine along with evaluation of urine methyl malonic
acid as a part of GCMS will also be studied in cases and controls.
Mother's blood samples will also be taken after explaining to them the entire aims and
objectives of the study and taking full free voluntary informed consent from them. This
would also benefit the mothers in a way because those identified with a deficiency will be
given supplements to overcome the deficiency.
Control samples will be taken from children of the same age group admitted for some other
ailment. A 2 ml sample will be taken from sample already drawn for other tests to be done in
the child. The controls also stand to be benefitted because all children found deficient
will be advised Vitamin B12 and folate supplementation immediately.
Vitamin B12 and folate levels will also be studied in the mothers of the 50 controls. All
mothers found deficient will be advised Vitamin B12 and folate.
