Generation of Haploid Stem Cells From Human Germ Cells

Induced Pluripotent Stem Cells Clinical Trial

Official title:

Generation of Haploid Stem Cells From Human Germ Cells

Clinical Trial Details — Status: Recruiting

Administrative data

• NCT number: NCT01454765
• Other study ID #: rmb-maza0001-ctil
• Status: Recruiting
• Phase: N/A
• First received: October 16, 2011
• Last updated: December 10, 2013
• Start date: December 2011
• Est. completion date: August 2015

Study information

• Verified date: December 2013
• Source: Rambam Health Care Campus
• Contact: n/a
• Is FDA regulated: No
• Health authority: Israel: Ethics Commission
• Study type: Observational

Clinical Trial Summary

Human cells are diploid (contain a double set of chromosomes). The diploid genome is complex and therefore limits genetic research approaches in biomedical models. To overcome this problem experimental generation of haploid stem cells has been done in animals. The investigators aim at generating haploid stem cells from human germ cells that are a useful tool in genetic research and screening.

Description:

Human cells are diploid (contain a double set of chromosomes). The diploid genome is complex and therefore limits genetic research approaches in biomedical models. To overcome this problem experimental generation of haploid stem cells has been done in animal models. haploid cells are easier for genetic manipulations, such as knock-in and knock-out of genes, and therefore should make a better experimental model. The investigators aim at generating haploid stem cells from human haploid germ cells.

Recruitment information / eligibility

• Status: Recruiting
• Enrollment: 10
• Est. completion date: August 2015
• Est. primary completion date: December 2014
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Male
• Age group: 18 Years to 50 Years

Eligibility

Inclusion Criteria:

1. males over the age of 18 and under 50

2. no known chronic illness

3. not taking any chronic drugs

Exclusion Criteria:

1. females

2. males under 18 or over 50

3. any known chronic illness

4. taking chronic medications

Study Design

Time Perspective: Prospective

Related Conditions & MeSH terms

• Induced Pluripotent Stem Cells

Intervention

Other:
• generation of stem cells
Collected germ cells will be cultured in medium. The germ cell cultures will be infected with reprograming factors. Rare colonies with characteristic stem cell morphologies will be expanded.

Locations

Country	Name	City	State
Israel	Rambam medical center	Haifa
Israel	Weizmann institute of science	Rehovot

Sponsors (1)

Lead Sponsor	Collaborator
Rambam Health Care Campus

Country where clinical trial is conducted

Israel, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Patients donated samples	No samples collected to date	June 2011-November 2013	No
Secondary	No secomdary outcome		June 2011 - November 2013	No