The Impact of Hypoxia on the Biochemical and Morphological Parameters of Blood in Rowers.

Hypoxia Clinical Trial

— IHOBMPBR  

Official title:

The Effects of Live High-train Low on EPO, VEGF, and Hematological Variables in Elite Rowers

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT06264193
• Other study ID #: Rowers
• Status: Completed
• Phase: N/A
• Start date: November 30, 2014
• Est. completion date: December 21, 2014

Study information

• Verified date: November 2023
• Source: Poznan University of Physical Education
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

The response to hypoxia is very individual and epending on many aspects, such as the type of training, duration, intensity, or hypoxic stimulus, hypoxia affects the athlete in various ways. The results of this study have shown that 18 days of the LH-TL method does not significantly increase the level of EPO and VEGF in rowers. However, reticulocytes, immature red blood cells, have shown significant differences after 18-d LH-TL between groups. Further research should be carried out to investigate an optimal hypoxic dose and time, which will raise EPO, VEGF, and morphology variables.

Description:

The Polish national rower team athletes, 13 males (ages 27,0±4,2 years), were randomly divided into two groups: 1) The hypoxic group (H) included 8 rowers participating in sports training and the LH-TL method, hypoxic rooms (FiO2=14.5%, corresponds to an altitude of 3000 meters). 2) The control group (C) presented 5 rowers participating only in sports training and living in normoxic rooms, at the same sports camp. Rowers were observed during the preparatory period. The hypoxic group lived and slept in hypoxic rooms, for at least 8-14 hours per day. During the camp, all rowers followed the same training schedule and diet.All participants' saturation and heart rate were measured daily, morning and evening, throughout the camp period. Venous blood samples were taken four times, at the beginning of the camp (Baseline), after 5 days, after 12 days, and after 18 days of the camp. Body composition parameters such as body mass index, body fat, and free fat mass were estimated using the bioelectrical impedance method of an analyzer Tanita BC 418 (Japan). Data analysis was performed using Statistica. The response to hypoxia is very individual. Depending on many aspects, such as the type of training, duration, intensity, or hypoxic stimulus, hypoxia affects the athlete in various ways. The results of this study have shown that the blood parameters did not increase significantly in the hypoxic group compared to the control group. However, reticulocytes, immature red blood cells, have shown significant differences after 18-d LH-TL between groups. Further research should be carried out to investigate an optimal hypoxic dose and time, which will raise EPO, VEGF, and morphology variables.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 13
• Est. completion date: December 21, 2014
• Est. primary completion date: December 21, 2014
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Male
• Age group: N/A and older

Eligibility

Inclusion Criteria:

• member of national rower's teams, and sports camp participant.

Exclusion Criteria:

• negative symptoms of hypoxia, and/or injury.

Related Conditions & MeSH terms

• Hypoxia

Intervention

Other:
• hypoxia
The athletes were living in hypoxic rooms, and training was carried out on lowlands.

Locations

Country	Name	City	State
n/a

Sponsors (1)

Lead Sponsor	Collaborator
Poznan University of Physical Education

References & Publications (4)

Czuba M, Fidos-Czuba O, Ploszczyca K, Zajac A, Langfort J. Comparison of the effect of intermittent hypoxic training vs. the live high, train low strategy on aerobic capacity and sports performance in cyclists in normoxia. Biol Sport. 2018 Mar;35(1):39-48 — View Citation

Kasperska A, Zembron-Lacny A. The effect of intermittent hypoxic exposure on erythropoietic response and hematological variables in elite athletes. Physiol Res. 2020 Apr 30;69(2):283-290. doi: 10.33549/physiolres.934316. Epub 2020 Mar 23. — View Citation

Ploszczyca K, Langfort J, Czuba M. The Effects of Altitude Training on Erythropoietic Response and Hematological Variables in Adult Athletes: A Narrative Review. Front Physiol. 2018 Apr 11;9:375. doi: 10.3389/fphys.2018.00375. eCollection 2018. — View Citation

Wilber RL. Application of altitude/hypoxic training by elite athletes. Med Sci Sports Exerc. 2007 Sep;39(9):1610-24. doi: 10.1249/mss.0b013e3180de49e6. — View Citation

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Erythropoietin (EPO) mlU/ml	immunoenzymatic assay methods using diagnostic kits	At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.
Primary	vascular endothelial growth factor (VEGF) mlU/ml	immunoenzymatic assay methods using diagnostic kits	At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.
Secondary	creatine kinase (CK) ng/ml	was determined using spectrophotometer analysis and diagnostic kits	At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.
Secondary	Hs C-Reactive Protein (hsCRP) mg/L	was determined by immunoenzymatic assay methods using diagnostic kits	At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.
Secondary	hemoglobin (Hb)g/dL	A professional accredited laboratory company determined the Hb level (Poland, ISO 9001:2008).	At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.
Secondary	hematocrit (Htc)%	A professional accredited laboratory company determined the Htc level (Poland, ISO 9001:2008).	At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.
Secondary	red blood cells (RBC)mln/mm3	A professional accredited laboratory company determined the RBC level (Poland, ISO 9001:2008).	At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.
Secondary	white blood cells (WBC)10 3/µL	A professional accredited laboratory company determined the WBC level (Poland, ISO 9001:2008).	At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.
Secondary	reticulocytes (Ret)‰	A professional accredited laboratory company determined the Ret level (Poland, ISO 9001:2008).	At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.
Secondary	SpO2 - oxygen saturation (%)	was measured using a pulse oximeter (Tech-Med, ISO: 13485).	every day, morning and evening, while sports camp
Secondary	bpm - beats per minute(%)	was measured using a pulse oximeter (Tech-Med, ISO: 13485).	every day, morning and evening, while sports camp

External Links

•   Exercise and Training at Altitudes: Physiological Effects and Protocols