High-intensity Interval Training Enhances Mobilization/Functionality of Endothelial Progenitor Cells and Depressed Shedding of Vascular Endothelial Cells Undergoing Hypoxia

Hypoxia Clinical Trial

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT02802462
• Other study ID #: 101-0408A3
• Status: Completed
• Phase: N/A
• First received: May 19, 2016
• Last updated: June 13, 2016
• Start date: December 2012
• Est. completion date: December 2013

Study information

• Verified date: June 2016
• Source: Chang Gung Memorial Hospital
• Contact: n/a
• Is FDA regulated: No
• Health authority: Taiwan: Ministry of Health and Welfare
• Study type: Interventional

Clinical Trial Summary

Exercise training improves endothelium-dependent vasodilation, whereas hypoxic stress causes vascular endothelial dysfunction. Monocyte- derived endothelial progenitor cells (Mon-EPCs) contribute to vascular repair process by differentiating into endothelial cells. This study investigates how high-intensity interval (HIT) and moderate intensity-continuous (MCT) exercise training affect circulating Mon-EPC levels and EPC functionality under hypoxic condition. Sixty healthy sedentary males were randomized to engage either HIT (3-minute intervals at 40% and 80%VO2max , n=20) or MCT (sustained 60%VO2max , n=20) for 30 minutes/day, 5 days/week for 6 weeks, or to a control group that did not received exercise intervention (n=20). Mon-EPC characteristics and EPC functionality under hypoxic exercise (HE, 100W under 12%O 2 ) were determined before and after various interventions.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 60
• Est. completion date: December 2013
• Est. primary completion date: December 2013
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Male
• Age group: 20 Years to 28 Years

Eligibility

Inclusion Criteria:

• Sedentary life

• Age 20~28

Exclusion Criteria:

• Smokers

• Users of medication/vitamins

• Any cardiopulmonary/hematological risk

• Regular exercise habits at least 1 year

• Exposed to high altitudes (>3000 m) for at least 1 year

Study Design

Allocation: Randomized, Intervention Model: Parallel Assignment, Masking: Open Label, Primary Purpose: Health Services Research

Related Conditions & MeSH terms

• Hypoxia

Intervention

Behavioral:
• HIT
3-minute intervals at 40% and 80%VO 2max
• MCT
sustained 60%VO 2max

Locations

Country	Name	City	State
n/a

Sponsors (1)

Lead Sponsor	Collaborator
Chang Gung Memorial Hospital

Outcome

Type	Measure	Description	Time frame	Safety issue
Other	Cardiac hemodynamic measurement	evaluate cardiac hemodynamic response to exercise by noninvasive continuous cardiac output monitoring system	6 weeks	No
Other	Cerebral hemodynamic measurement	Two pairs of near infrared probes were attached to each subject to monitor the absorption of near infrared light across left frontal cortex during graded exercise test	6 weeks	No
Other	Muscular hemodynamic measurement	Two pairs of near infrared probes were attached to each subject to monitor the absorption of near infrared light across left vastus lateralis muscle during graded exercise test	6 weeks	No
Primary	Circulating progenitor cells conten	by flow cytometry before and after rehabilitation circulating progenitor cells conten before and after hypoxic exercise test	6 weeks	No
Secondary	Cardiopulmonary fitness		6 weeks	No