Outcome
Type |
Measure |
Description |
Time frame |
Safety issue |
Primary |
Fluoride Concentration in Dental Biofilm Fluid at Baseline |
Dental biofilm was collected at baseline (before mouthwash(es)), and the fluid phase was separated using centrifugation. Fluoride concentration in the dental biofilm fluid was measured using an ion-specific electrode adapted for microanalysis. |
Baseline |
|
Primary |
Fluoride Concentration in Dental Biofilm Fluid at 15 Minutes |
Dental biofilm was collected 15 minutes after mouthwash(es), and the fluid phase was separated using centrifugation. Fluoride concentration in the dental biofilm fluid was measured using an ion-specific electrode adapted for microanalysis. |
15 minutes after mouthwash(es) |
|
Primary |
Fluoride Concentration in Dental Biofilm Fluid at 60 Minutes |
Dental biofilm was collected 60 minutes after mouthwash(es), and the fluid phase was separated using centrifugation. Fluoride concentration in the dental biofilm fluid was measured using an ion-specific electrode adapted for microanalysis. |
60 minutes after mouthwash(es) |
|
Primary |
Fluoride Concentration in Dental Biofilm Fluid at 120 Minutes |
Dental biofilm was collected 120 minutes after mouthwash(es), and the fluid phase was separated using centrifugation. Fluoride concentration in the dental biofilm fluid was measured using an ion-specific electrode adapted for microanalysis. |
120 minutes after mouthwash(es) |
|
Primary |
Fluoride Concentration in Dental Biofilm Solids at Baseline |
Dental biofilm was collected at baseline (before mouthwash(es)). The fluid phase of the biofilm was collected after centrifugation, and the remaining solids were treated with strong acid for dissolution of fluoride reservoirs. Solids remaining after acid extraction were treated with strong alkali for total dissolution of the biofilm and estimation of biofilm mass by protein concentration. Fluoride concentration in the dental biofilm solids acid extract was measured using an ion-specific electrode adapted for microanalysis; protein concentration in the alkali extract was determined using the Lowry colorimetric method. Fluoride concentration in the biofilm was expressed as micrograms of fluoride/milligrams of protein. |
Baseline |
|
Primary |
Fluoride Concentration in Dental Biofilm Solids at 15 Minutes |
Dental biofilm was collected 15 minutes after mouthwash(es). The fluid phase of the biofilm was collected after centrifugation, and the remaining solids were treated with strong acid for dissolution of fluoride reservoirs. Solids remaining after acid extraction were treated with strong alkali for total dissolution of the biofilm and estimation of biofilm mass by protein concentration. Fluoride concentration in the dental biofilm solids acid extract was measured using an ion-specific electrode adapted for microanalysis; protein concentration in the alkali extract was determined using the Lowry colorimetric method. Fluoride concentration in the biofilm was expressed as micrograms of fluoride/milligrams of protein. |
15 minutes after mouthwash(es) |
|
Primary |
Fluoride Concentration in Dental Biofilm Solids at 60 Minutes |
Dental biofilm will be collected 60 minutes after mouthwash(es). The fluid phase of the biofilm was collected after centrifugation, and the remaining solids were treated with strong acid for dissolution of fluoride reservoirs. Solids remaining after acid extraction were treated with strong alkali for total dissolution of the biofilm and estimation of biofilm mass by protein concentration. Fluoride concentration in the dental biofilm solids acid extract was measured using an ion-specific electrode adapted for microanalysis; protein concentration in the alkali extract was determined using the Lowry colorimetric method. Fluoride concentration in the biofilm was expressed as micrograms of fluoride/milligrams of protein. |
60 minutes after mouthwash(es) |
|
Primary |
Fluoride Concentration in Dental Biofilm Solids at 120 Minutes |
Dental biofilm was collected 120 minutes after mouthwash(es). The fluid phase of the biofilm was collected after centrifugation, and the remaining solids were treated with strong acid for dissolution of fluoride reservoirs. Solids remaining after acid extraction were treated with strong alkali for total dissolution of the biofilm and estimation of biofilm mass by protein concentration. Fluoride concentration in the dental biofilm solids acid extract was measured using an ion-specific electrode adapted for microanalysis; protein concentration in the alkali extract was determined using the Lowry colorimetric method. Fluoride concentration in the biofilm was expressed as micrograms of fluoride/milligrams of protein. |
120 minutes after mouthwash(es) |
|
Primary |
Area Under the Curve of Fluoride Concentration in Dental Biofilm Fluid Overtime |
Fluoride concentration in dental biofilm fluid collected at baseline (before mouthwash(es)), 15, 60 and 120 minutes after mouthwash(es) was expressed as the area under the curve of fluoride concentration in dental biofilm fluid as a function of time (parts per million x min). |
Baseline, 15, 60 and 120 minutes after mouthwash(es) |
|
Primary |
Area Under the Curve of Fluoride Concentration in Dental Biofilm Solids Overtime |
Fluoride concentration in dental biofilm solids collected at baseline (before mouthwash(es)), 15, 60 and 120 minutes after mouthwash(es) was expressed as the area under the curve of fluoride concentration in dental biofilm solids as a function of time (micrograms of fluoride x minutes/ milligram of proteins). |
Baseline, 15, 60 and 120 minutes after mouthwash |
|
Secondary |
Fluoride Concentration in Saliva at Baseline |
Unstimulated saliva was collected at baseline (before mouthwash(es)), for 5 minutes. Fluoride concentration in unstimulated saliva was measured using an ion-specific electrode adapted for microanalysis. |
Baseline |
|
Secondary |
Fluoride Concentration in Saliva at 16 Minutes |
Unstimulated saliva was collected 16 minutes after mouthwash(es), for 5 minutes. Fluoride concentration in unstimulated saliva was measured using an ion-specific electrode adapted for microanalysis. |
16-21 minutes after mouthwash(es) |
|
Secondary |
Fluoride Concentration in Saliva at 30 Minutes |
Unstimulated saliva was collected 30 minutes after mouthwash(es), for 5 minutes. Fluoride concentration in unstimulated saliva was measured using an ion-specific electrode adapted for microanalysis. |
30-35 minutes after mouthwash(es) |
|
Secondary |
Fluoride Concentration in Saliva at 61 Minutes |
Unstimulated saliva was collected 61 minutes after mouthwash(es), for 5 minutes. Fluoride concentration in unstimulated saliva was measured using an ion-specific electrode adapted for microanalysis. |
61-66 minutes after mouthwash(es) |
|
Secondary |
Fluoride Concentration in Saliva at 90 Minutes |
Unstimulated saliva was collected 90 minutes after mouthwash(es), for 5 minutes. Fluoride concentration in unstimulated saliva was measured using an ion-specific electrode adapted for microanalysis. |
90-95 minutes after mouthwash(es) |
|
Secondary |
Fluoride Concentration in Saliva at 121 Minutes |
Unstimulated saliva was collected 121 minutes after mouthwash(es), for 5 minutes. Fluoride concentration in unstimulated saliva was measured using an ion-specific electrode adapted for microanalysis. |
121-126 minutes after mouthwash(es) |
|
Secondary |
Calcium Concentration in Dental Biofilm Fluid at Baseline |
Dental biofilm will be collected at baseline (before mouthwash(es)), and the fluid phase will be separated using centrifugation. Calcium concentration in the dental biofilm fluid will be measured using an ion-specific electrode adapted for microanalysis, and will be expressed in millimolar. |
Baseline |
|
Secondary |
Calcium Concentration in Dental Biofilm Fluid at 15 Minutes |
Dental biofilm will be collected 15 minutes after mouthwash(es), and the fluid phase will be separated using centrifugation. Calcium concentration in the dental biofilm fluid will be measured using an ion-specific electrode adapted for microanalysis, and will be expressed in millimolar. |
15 minutes after mouthwash(es) |
|
Secondary |
Calcium Concentration in Dental Biofilm Fluid at 60 Minutes |
Dental biofilm will be collected 60 minutes after mouthwash(es), and the fluid phase will be separated using centrifugation. Calcium concentration in the dental biofilm fluid will be measured using an ion-specific electrode adapted for microanalysis, and will be expressed in millimolar. |
60 minutes after mouthwash(es) |
|
Secondary |
Calcium Concentration in Dental Biofilm Fluid at 120 Minutes |
Dental biofilm will be collected 120 minutes after mouthwash(es), and the fluid phase will be separated using centrifugation. Calcium concentration in the dental biofilm fluid will be measured using an ion-specific electrode adapted for microanalysis, and will be expressed in millimolar. |
120 minutes after mouthwash(es) |
|
Secondary |
Calcium Concentration in Dental Biofilm Solids at Baseline |
Dental biofilm was collected at baseline (before mouthwash(es)). The fluid phase of the biofilm was collected after centrifugation, and the remaining solids were treated with strong acid for dissolution of calcium reservoirs. Solids remaining after acid extraction were treated with strong alkali for total dissolution of the biofilm and estimation of biofilm mass by protein concentration. Calcium concentration in the dental biofilm solids acid extract was measured using a colorimetric reaction (Arsenazo III); protein concentration in the alkali extract was determined using the Lowry colorimetric method. Calcium concentration in the biofilm was expressed as micrograms of calcium/milligrams of protein. |
Baseline |
|
Secondary |
Calcium Concentration in Dental Biofilm Solids at 15 Minutes |
Dental biofilm was collected 15 minutes after mouthwash(es). The fluid phase of the biofilm was collected after centrifugation, and the remaining solids were treated with strong acid for dissolution of calcium reservoirs. Solids remaining after acid extraction were treated with strong alkali for total dissolution of the biofilm and estimation of biofilm mass by protein concentration. Calcium concentration in the dental biofilm solids acid extract was measured using a colorimetric reaction (Arsenazo III); protein concentration in the alkali extract was determined using the Lowry colorimetric method. Calcium concentration in the biofilm was expressed as micrograms of calcium/milligrams of protein. |
15 minutes after mouthwash(es) |
|
Secondary |
Calcium Concentration in Dental Biofilm Solids at 60 Minutes |
Dental biofilm was collected 60 minutes after mouthwash(es). The fluid phase of the biofilm was collected after centrifugation, and the remaining solids were treated with strong acid for dissolution of calcium reservoirs. Solids remaining after acid extraction were treated with strong alkali for total dissolution of the biofilm and estimation of biofilm mass by protein concentration. Calcium concentration in the dental biofilm solids acid extract was measured using a colorimetric reaction (Arsenazo III); protein concentration in the alkali extract was determined using the Lowry colorimetric method. Calcium concentration in the biofilm was expressed as micrograms of calcium/milligrams of protein. |
60 minutes after mouthwash(es) |
|
Secondary |
Calcium Concentration in Dental Biofilm Solids at 120 Minutes |
Dental biofilm was collected 120 minutes after mouthwash(es). The fluid phase of the biofilm was collected after centrifugation, and the remaining solids were treated with strong acid for dissolution of calcium reservoirs. Solids remaining after acid extraction were treated with strong alkali for total dissolution of the biofilm and estimation of biofilm mass by protein concentration. Calcium concentration in the dental biofilm solids acid extract was measured using a colorimetric reaction (Arsenazo III); protein concentration in the alkali extract was determined using the Lowry colorimetric method. Calcium concentration in the biofilm was expressed as micrograms of calcium/milligrams of protein. |
120 minutes after mouthwash(es) |
|
Secondary |
Calcium Concentration in Saliva at Baseline |
Unstimulated saliva was collected at baseline (before mouthwash(es)), for 5 minutes. Calcium concentration in unstimulated saliva was measured using a colorimetric reaction (Arsenazo III). |
Baseline |
|
Secondary |
Calcium Concentration in Saliva at 16 Minutes |
Unstimulated saliva was collected at 16 minutes after mouthwash(es), for 5 minutes. Calcium concentration in unstimulated saliva was measured using a colorimetric reaction (Arsenazo III). |
16-21 minutes after mouthwash(es) |
|
Secondary |
Calcium Concentration in Saliva at 30 Minutes |
Unstimulated saliva was collected at 30 minutes after mouthwash(es), for 5 minutes. Calcium concentration in unstimulated saliva was measured using a colorimetric reaction (Arsenazo III). |
30-35 minutes after mouthwash(es) |
|
Secondary |
Calcium Concentration in Saliva at 61 Minutes |
Unstimulated saliva was collected at 61 minutes after mouthwash(es), for 5 minutes. Calcium concentration in unstimulated saliva was measured using a colorimetric reaction (Arsenazo III). |
61-66 minutes after mouthwash(es) |
|
Secondary |
Calcium Concentration in Saliva at 90 Minutes |
Unstimulated saliva was collected at 90 minutes after mouthwash(es), for 5 minutes. Calcium concentration in unstimulated saliva was measured using a colorimetric reaction (Arsenazo III). |
90-95 minutes after mouthwash(es) |
|
Secondary |
Calcium Concentration in Saliva at 121 Minutes |
Unstimulated saliva was collected at 121 minutes after mouthwash(es), for 5 minutes. Calcium concentration in unstimulated saliva was measured using a colorimetric reaction (Arsenazo III). |
121-126 minutes after mouthwash(es) |
|
Secondary |
Area Under the Curve of Fluoride Concentration in Saliva Overtime |
Fluoride concentration in unstimulated saliva collected at baseline (before mouthwash(es)), 16, 30, 61, 90 and 121 minutes after mouthwash(es) was expressed as the area under the curve of fluoride concentration in saliva as a function of time. |
Baseline, 16, 30, 61, 90 and 121 minutes after mouthwash(es) |
|
Secondary |
Area Under the Curve of Calcium Concentration in Dental Biofilm Fluid Overtime |
Calcium concentration in dental biofilm fluid collected at baseline (before mouthwash(es)), 15, 60 and 120 minutes after mouthwash(es) will be expressed as the area under the curve of calcium concentration in dental biofilm fluid as a function of time (millimolar x min). |
Baseline, 15, 60 and 120 minutes after mouthwash(es) |
|
Secondary |
Area Under the Curve of Calcium Concentration in Dental Biofilm Solids Overtime |
Calcium concentration in dental biofilm solids collected at baseline (before mouthwash(es)), 15, 60 and 120 minutes after mouthwash(es) was expressed as the area under the curve of calcium concentration in dental biofilm solids as a function of time. |
Baseline, 15, 60 and 120 minutes after mouthwash(es) |
|
Secondary |
Area Under the Curve of Calcium Concentration in Saliva Overtime |
Calcium concentration in unstimulated saliva collected at baseline (before mouthwash(es)), 16, 30, 61, 90 and 121 minutes after mouthwash(es) was expressed as the area under the curve of calcium concentration in saliva as a function of time (millimolar x min). |
Baseline, 16, 30, 61, 90 and 121 minutes after a mouthwash(es) |
|