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Clinical Trial Details — Status: Completed

Administrative data

NCT number NCT04596553
Other study ID # H20-00943
Secondary ID
Status Completed
Phase N/A
First received
Last updated
Start date January 25, 2021
Est. completion date February 13, 2022

Study information

Verified date June 2023
Source University of British Columbia
Contact n/a
Is FDA regulated No
Health authority
Study type Interventional

Clinical Trial Summary

The purpose of this study is to assess which amino acids are affecting acute muscle collagen synthesis in response to supplementation and exercise.


Description:

This is a double-blinded, randomized controlled, parallel design study, with 3 treatment groups (placebo, collagen peptide, and essential amino acid). The study will involve 3 visits: a baseline testing/familiarization session, and 2 exercise trials which will include muscle biopsies, blood draws, and the infusion of a tracer isotope in order to measure muscle protein synthesis.


Recruitment information / eligibility

Status Completed
Enrollment 17
Est. completion date February 13, 2022
Est. primary completion date February 13, 2022
Accepts healthy volunteers Accepts Healthy Volunteers
Gender Male
Age group 18 Years to 35 Years
Eligibility Inclusion Criteria: - Can fluently read and write in English - Male - 18 - 35 years Exclusion Criteria: - Diabetes, cardiovascular disease, or current pharmaceutical therapy - Medical conditions impacting ability to undertake strenuous physical activity - Lower body resistance training in the previous 6 months - BMI > 30 - Currently smoking with cigarettes - Allergy to any study supplements - Currently taking any protein, amino acid, or collagen supplement - Participate in greater than 2 hours of weekly vigorous exercise

Study Design


Intervention

Dietary Supplement:
Essential Amino Acid
Immediately following the exercise intervention participants will consume their assigned essential amino acid supplement
Collagen Peptide
Immediately following the exercise intervention participants will consume their assigned collagen peptide supplement
Maltodextrin Placebo
Immediately following the exercise intervention participants will consume their assigned maltodextrin supplement

Locations

Country Name City State
Canada Chan Gunn Pavilion Vancouver British Columbia

Sponsors (1)

Lead Sponsor Collaborator
University of British Columbia

Country where clinical trial is conducted

Canada, 

References & Publications (7)

Alcock RD, Shaw GC, Tee N, Burke LM. Plasma Amino Acid Concentrations After the Ingestion of Dairy and Collagen Proteins, in Healthy Active Males. Front Nutr. 2019 Oct 15;6:163. doi: 10.3389/fnut.2019.00163. eCollection 2019. — View Citation

Egerman MA, Glass DJ. Signaling pathways controlling skeletal muscle mass. Crit Rev Biochem Mol Biol. 2014 Jan-Feb;49(1):59-68. doi: 10.3109/10409238.2013.857291. Epub 2013 Nov 18. — View Citation

Frontera WR, Ochala J. Skeletal muscle: a brief review of structure and function. Calcif Tissue Int. 2015 Mar;96(3):183-95. doi: 10.1007/s00223-014-9915-y. Epub 2014 Oct 8. — View Citation

Holm L, Rahbek SK, Farup J, Vendelbo MH, Vissing K. Contraction mode and whey protein intake affect the synthesis rate of intramuscular connective tissue. Muscle Nerve. 2017 Jan;55(1):128-130. doi: 10.1002/mus.25398. Epub 2016 Sep 26. — View Citation

Holm L, van Hall G, Rose AJ, Miller BF, Doessing S, Richter EA, Kjaer M. Contraction intensity and feeding affect collagen and myofibrillar protein synthesis rates differently in human skeletal muscle. Am J Physiol Endocrinol Metab. 2010 Feb;298(2):E257-69. doi: 10.1152/ajpendo.00609.2009. Epub 2009 Nov 10. — View Citation

Miller BF, Olesen JL, Hansen M, Dossing S, Crameri RM, Welling RJ, Langberg H, Flyvbjerg A, Kjaer M, Babraj JA, Smith K, Rennie MJ. Coordinated collagen and muscle protein synthesis in human patella tendon and quadriceps muscle after exercise. J Physiol. 2005 Sep 15;567(Pt 3):1021-33. doi: 10.1113/jphysiol.2005.093690. Epub 2005 Jul 7. — View Citation

Shaw G, Lee-Barthel A, Ross ML, Wang B, Baar K. Vitamin C-enriched gelatin supplementation before intermittent activity augments collagen synthesis. Am J Clin Nutr. 2017 Jan;105(1):136-143. doi: 10.3945/ajcn.116.138594. Epub 2016 Nov 16. — View Citation

Outcome

Type Measure Description Time frame Safety issue
Primary Fractional synthetic rate (change over time) of Myofibrillar Protein Synthesis Fraction of the myofibrillar enriched protein from muscle biopsy per unit time. 0 - 4 hours post-exercise
Primary Fractional synthetic rate (change over time) of Myofibrillar Protein Synthesis Fraction of the myofibrillar enriched protein from muscle biopsy per unit time. 0 - 2 hours post-exercise
Primary Fractional synthetic rate (change over time) of Myofibrillar Protein Synthesis Fraction of the myofibrillar enriched protein from muscle biopsy per unit time. 2 - 4 hours post-exercise
Primary Fractional synthetic rate (change over time) Muscle Collagen Synthesis Fraction of the intramuscular collagen enriched protein from muscle biopsy per unit time. 0 - 4 hours post-exercise
Primary Fractional synthetic rate (change over time) Muscle Collagen Synthesis Fraction of the intramuscular collagen enriched protein from muscle biopsy per unit time. 0 - 2 hours post-exercise
Primary Fractional synthetic rate (change over time) Muscle Collagen Synthesis Fraction of the intramuscular collagen enriched protein from muscle biopsy per unit time. 2 - 4 hours post-exercise
Primary Pro-Collagen Type I N-Propeptide (PINP) Osteoblastic marker of bone collagen synthesis 1 hour prior to exercise
Primary Pro-Collagen Type I N-Propeptide (PINP) Osteoblastic marker of bone collagen synthesis 4 hours post exercise
Secondary Plasma Amino Acids Response to Supplementation Amino acid plasma concentrations 0 - 4 hours post-exercise
Secondary Muscle mechanistic target of rapamycin Pathway Activation Measuring the fold change in enzymes associated with the anabolic signalling of the mTOR pathway (e.g. phosphoinositide 3-kinases (PI3k), protein kinase B (Akt)) 0 - 4 hours post-exercise
Secondary PCR Gene Expression of Muscle Collagen Genes (Types I, III, IV) Measuring the fold change when the collagen genes of interest become detectable in the PCR analysis 0 - 4 hours post-exercise
Secondary Pro-Collagen Type III N-Propeptide (PIIINP) Marker of collagen synthesis 1 hour prior to exercise
Secondary Pro-Collagen Type III N-Propeptide (PIIINP) Marker of collagen synthesis 4 hours post exercise
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