Exercise Clinical Trial
Official title:
Effects of Acute Exercise on (Myokine) Gene Expression in Human Skeletal Muscle
Rationale: Proteins released from muscle during and shortly after exercise, often referred
to as myokines, may be central to our understanding of the cross-talk during and after
exercise between skeletal muscles and other organs, in particular the liver. So far only a
few myokines are identified (e.g. IL-6, IL-8, IL-15, TNF-alpha). Taking into account the
role of these several known myokines in developing insulin resistance, revealing new
putative myokines might provide valuable information and a direction for future research on
the pathogenesis and treatment of type 2 diabetes mellitus.
Objective: The objective of the present study is to identify novel myokines, expression of
which is altered in skeletal muscle after a single bout of exercise.
Study design: experimental study. Study population: Ten healthy, male subjects between 40
and 60 years of age and BMI < 30 kg/m2, will participate in this study.
Intervention: A single exercise bout that consists of one hour one-legged cycling on a
adapted recumbent cycle ergometer at a submaximal rate. The non-exercising leg will serve as
control for the exercising leg.
Main study outcomes: Main study outcomes include upregulation of genes in skeletal muscle
after exercise (with a focus on genes encoding myokines) and changes of blood plasma levels
of selected proteins after exercise.
Status | Completed |
Enrollment | 12 |
Est. completion date | June 2011 |
Est. primary completion date | June 2011 |
Accepts healthy volunteers | Accepts Healthy Volunteers |
Gender | Male |
Age group | 40 Years to 60 Years |
Eligibility |
Inclusion Criteria: - Age 40-60 years - Male gender - BMI < 30 kg/m2 Exclusion Criteria: - Exercising regularly (> 2 times a week, > 3 hour in total per week) - Unstable body weight (weight gain or loss > 3 kg in the past three months) - Following, or have recently followed a (weight-loss) diet - Donated or intended to donate blood 2 months before until two months after the study - Medical condition that can interfere with the study outcome (i.e. cardiovascular disease, pulmonary disease) - Systolic blood pressure >160 mmHg and/or diastolic blood pressure >100 mmHg - Use of medications known to interfere with gene expression in the muscles (i.e. statins, fenofibrate). - Use of antithrombotic therapy (marcoumar, sintromitis). - Diagnosed diabetes mellitus type 1 or 2. - Drugs or alcohol abuse ( > 3 glasses of alcoholic beverages a day). - (Chronic) injuries of the locomotor system that can interfere with the intervention - Participated in another study within the last six months |
Intervention Model: Single Group Assignment, Masking: Open Label, Primary Purpose: Basic Science
Country | Name | City | State |
---|---|---|---|
Netherlands | Division of Human Nutrition, Wageningen University | Wageningen | Gelderland |
Lead Sponsor | Collaborator |
---|---|
Wageningen University | Dutch Diabetes Research Foundation |
Netherlands,
Type | Measure | Description | Time frame | Safety issue |
---|---|---|---|---|
Primary | Gene expression changes in skeletal muscle from baseline to after exercise | Gene expression is measured using whole genome Affymatrix microarrays Muscle tissue will be collected at time points T = 0 (before exercise) and T = 1 (directly after exercise). Special focus is on the changes in genes coding for myokines. Up to 5 genes will be selected for follow-up analysis. | T = 0 and T =1 | No |
Primary | Changes in plasma levels of selected proteins | Selected proteins will be analyzed by ELISA assays or western blot analysis, depending on ELISA availability at T = 0, T = 1 and T = 3 (2 hours post-exercise). The selection of the proteins is based on significance, the robustness of induction (>80% of subjects showing induction) and the magnitude of the induction ( mean fold change > 2) | T = 0, T =1 and T = 3 | No |
Secondary | PBMC gene expression changes before and after exercise | Gene expression changes in the blood will be assessed using whole genome Affymetrix microarrays for the samples at T = 0, 1 and 3. | T = 0, T = 1 and T =3 | No |
Secondary | Routine plasma level changes before and after exercise | At all three time point (T = 0, 1 and 3) plasma levels of glucose, insulin, fatty acids, triglycerides, cortisol, adrenalin and lactate will be determined. | T = 0, 1 and 3 | No |
Secondary | Heart rate changes, baseline compared with exercise | Heart rate will be measured during the maximum work load test and the experimental exercise period. | During the intervention | No |
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