The Association Between Food Timing and Composition on Gastrointestinal Injury, Stress Hormone and Gastrointestinal Complains.

Endothelial Dysfunction Clinical Trial

Official title:

The Association Between Food Timing and Composition on Gastrointestinal Injury, Stress Hormone and Gastrointestinal Complains in Elite Rowers After 2000m Ergometer Test - an Observational Study.

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT06403241
• Other study ID #: Rowerscometetive2023
• Status: Completed
• Start date: May 1, 2023
• Est. completion date: June 30, 2023

Study information

• Verified date: June 2023
• Source: Poznan University of Physical Education
• Contact: n/a
• Is FDA regulated: No
• Study type: Observational

Clinical Trial Summary

The study aimed to check the influence of timing and composition of diet on markers of gut injury, endotoxemia and stress.

Description:

The research protocol includes detailed food intake analysis, including timing and composition, markers related to the neuroendocrine pathway cortisol, and observations of individually experienced symptoms. Moreover, the study also explores intestinal injury and endotoxemia parameters, such as I-FABP, CLDN-3, and LBP. As a first goal, this study investigates the timing and composition of food intake concerning changes in gut integrity, cortisol levels, and gastrointestinal symptoms. Investigators explored whether an athlete's nutritional intake influences any GI disturbance induced by high-intensity exercise. As a second goal, Investigators tried to connect symptoms and markers from both pathways with gastrointestinal complaints. Investigators hypothesised that cortisol levels would be more profound than other studied parameters in gastrointestinal symptoms.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 13
• Est. completion date: June 30, 2023
• Est. primary completion date: June 1, 2023
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Male
• Age group: 18 Years to 23 Years

Eligibility

Inclusion Criteria:

• Membership in Polish Rowing Team,

• minimum five years of training,

• total training time minimum of 240 minutes,

• filling out a food diary,

• finishing 2000-meter ergometer test.

Exclusion Criteria:

• Probiotics within the last three months,

• prebiotics within the last three months

• antibiotic therapy within the last three months,

• dietary regime,

• gastrointestinal diseases,

• lactose intolerance

Related Conditions & MeSH terms

• Endothelial Dysfunction

Intervention

Other:
• 2000 meter ergometer test
Rowers performed 2000m ergometer test.A 2,000-m time trial is a standard test used to assess performance in rowers.

Locations

Country	Name	City	State
Poland	Poznan University of Physical Education, Faculty of Sport Sciences in Gorzow	Gorzów Wielkopolski	Lubuskie

Sponsors (1)

Lead Sponsor	Collaborator
Poznan University of Physical Education

Country where clinical trial is conducted

Poland, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	I-FABP (intestinal fatty acid binding protein)to measure epithelial wall injury	I-FABP concentration measured in blood [ng/ml] using commercially available enzyme-linked immunosorbent assays	Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 1-hour recovery period
Primary	Claudin to measure tight junction leakage	Zonulin concentration measured in blood [ng/ml] using commercially available enzyme-linked immunosorbent assays	Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 1-hour recovery period
Primary	LBP (lipopolysaccharide binding protein) to measure endotoxin	LBP concentration measured in blood [ug/ml] using commercially available enzyme-linked immunosorbent assays	Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 1-hour recovery period
Primary	Lactic acid to measure fatigue after the race	Lactate concentration measured in capillary blood from earlobe [mmol/L] using a commercially available kit (Diaglobal, Berlin, Germany)	Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test
Primary	Corisol	Stress hormoneconcentration measured in blood [ng/ml] using commercially available enzyme-linked immunosorbent assays	Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 1-hour recovery period
Secondary	energy	energy [kcal ]of food intake measured by food diary	whole day (24hours) before test, in the morning before the test
Secondary	protein	protein [g ]of food intake measured by food diary	whole day (24hours) before test, in the morning before the test
Secondary	fat	fat [g ]of food intake measured by food diary	whole day (24hours) before test, in the morning before the test
Secondary	carbohydrate	carbohydrate [g ]of food intake measured by food diary	whole day (24hours) before test, in the morning before the test
Secondary	glucose	glucose [g ]of food intake measured by food diary	whole day (24hours) before test, in the morning before the test
Secondary	lactose	lactose [g ]of food intake measured by food diary	whole day(24hours) before test, in the morning before the test
Secondary	saccharose	saccharose [g ]of food intake measured by food diary	whole day (24hours) before test, in the morning before the test
Secondary	fructose	fructose [g ]of food intake measured by food diary	whole day (24hours) before test, in the morning before the test
Secondary	fiber	fiber [g ]of food intake measured by food diary	whole day (24hours) before test, in the morning before the test
Secondary	Body mass	Body mass [kg] measured by electronic scale to the nearest 0,05 kg (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).	in the morning before the test up to 1 hour after getting up
Secondary	Body fat	Body fat [%] measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).	in the morning before the test up to 1 hour after getting up
Secondary	Total body water	Body fat [%] measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).	in the morning before the test up to 1 hour after getting up
Secondary	Lean body mass	Lean body mass [kg] measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).	in the morning before the test up to 1 hour after getting up
Secondary	height	[cm] measured by high meter	in the morning before the test up to 1 hour after getting up
Secondary	self reported gastrointestinal scale	10 point scale	up to 1 hours after the test