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Comparison of the Methylation Pattern of DNA in Buccal Swabs From Different Time Points

DNA Methylation Clinical Trial

Official title:
Comparison of the Methylation Pattern of DNA in Buccal Swabs From Different Time Points

Clinical Trial Summary

A buccal swab will be taken using a cotton stick by rubbing it against the buccal mucosa. Samples will be taken from the same participant at different timepoints.

DNA will be extracted from the buccal cells on the swabs using a commercial extraction kit and will be quantified by a nano-drop spectrophotometer.

We will determine global and gene-specific methylation and hydroxymethylation alterations by UPLC/MS/MS. Hydrolyzed DNA into individual nucleosides will be analyzed for the quantitative measurement of 5-methylCytosine and 5-hydroxymethylCytosine on Triple Quadrupole UPLC/MS/MS platform. Secondly, specific methylation in the CpG islands of tumor suppressor and promoter genes, and genes involved in the oxidative stress pathways will be assessed by PCR-pyrosequencing.

Clinical Trial Description

n/a

Study Design

Observational Model: Cohort, Time Perspective: Prospective

Related Conditions & MeSH terms

Clinical Trial Details
NCT number NCT02297009
Study type Observational
Source Universitaire Ziekenhuizen Leuven
Contact Kirsten Van Landuyt, Prof. Dr
Email kirsten.vanlanduyt@med.kuleuven.be
Status Recruiting
Phase N/A
Start date December 2014
Completion date September 2018
View Details
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