Clinical Trial Details
— Status: Active, not recruiting
Administrative data
| NCT number |
NCT05052827 |
| Other study ID # |
36378 |
| Secondary ID |
|
| Status |
Active, not recruiting |
| Phase |
N/A
|
| First received |
|
| Last updated |
|
| Start date |
September 1, 2021 |
| Est. completion date |
March 2024 |
Study information
| Verified date |
November 2023 |
| Source |
University of Toronto |
| Contact |
n/a |
| Is FDA regulated |
No |
| Health authority |
|
| Study type |
Interventional
|
Clinical Trial Summary
Leucine-enriched proteins (e.g., dairy) are more effective than plant-based sources for
supporting post-exercise rates of muscle protein synthesis in adults. However, the impact of
protein quality on protein synthesis and acute markers of growth in active children are
unknown. Using a randomized crossover design, the investigators will compare the effects of
ingesting 0.35g/kg fat free mass (FFM) of either milk protein concentrate (MPC), rice protein
isolate (RPI), or soy protein isolate (SPI) following a bout of variable intensity exercise
with whole-body protein kinetics (i.e., oxidation, synthesis, breakdown, and net balance)
determined by oral [13C]phenylalanine tracer methodology.
Description:
In adults, dairy protein is more effective than non-dairy sources at supporting recovery from
exercise; however, it is not well understood if protein quality impacts short-term markers of
growth in the same manner in active youth. Therefore, it is essential to understand whether
different anabolic responses to varying protein quality persist in children, in order to
maximize lean tissue accretion specifically during these periods of exponential growth. Thus,
assessing the impact of protein quality on post-exercise protein synthesis and whole-body
protein balance in active children would help provide valuable maturation-specific
nutritional information. Moreover, these studies investigating the effects of protein quality
in adults required the use of invasive techniques (i.e., muscle biopsies and indwelling
catheters for blood draws) and are not suitable for vulnerable populations like children. As
a result, the study of protein quality in children requires non-invasive alternative
approaches and novel methods to advance our understanding of the nutritional best practices
for active youth.
The overall objective of this investigation is to examine the impact of protein quality on
post-exercise protein synthesis and acute markers of "growth" (i.e., net protein balance) in
active youth (children and adolescents). Using the minimally invasive indicator amino acid
oxidation (IAAO) methodology, we will determine the ability of milk, rice, and soy proteins
to support whole-body protein synthesis and net-protein balance during recovery from an acute
bout of sport specific moderate-to-vigorous physical activity.
The primary aim of the present study is to compare the ability of dairy and non-dairy
alternatives (i.e., plant-based protein sources) to support post-exercise protein synthesis
and net-protein balance in active, growing youth. The investigators hypothesize that the
ingestion of milk protein concentrate (MPC) will result in a lower indicator amino acid
oxidation (i.e., greatest protein synthesis) and higher net protein balance after exercise in
both children and adolescents, compared to an isonitrogenous (i.e., equal protein amount)
quantity of soy (SPI) and rice (RPI) protein isolate. This is predicated on the relative
deficiency of branched-chain amino acids (BCAA) and other essential amino acids (EAA),
specifically lysine and methionine, in RPI and SPI respectively, compared to MPC.
