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Clinical Trial Details — Status: Recruiting

Administrative data

NCT number NCT05711199
Other study ID # NEXT Study
Secondary ID
Status Recruiting
Phase Early Phase 1
First received
Last updated
Start date March 1, 2023
Est. completion date August 31, 2025

Study information

Verified date May 2024
Source University Hospital, Basel, Switzerland
Contact Matthias J Betz, MD
Phone 0041 61 556 56 54
Email matthias.betz@usb.ch
Is FDA regulated No
Health authority
Study type Interventional

Clinical Trial Summary

The aim of this study is to evaluate the efficacy of glyceroltrinitrate (Nitroderm® TTS) to activate and expand human BAT as compared to mild cold exposure.


Description:

The activation of brown adipose tissue in response to glyceroltrinitrate as compared to cold exposure will be studied in an open-label, cross-over trial in healthy volunteers with random sequence of the two study periods (treatment and control) seperated by a washout period of 7 days. In the treatment phase all participants will receive Nitroderm® TTS (glyceroltrinitrate) for a total of 15 days. For better tolerability, it will be will started with a lower dose of 5mg/24h (Nitroderm® TTS) the first 5 days and after that a change to Nitroderm® TTS 10mg/24h for another 10 days will be done. Each transdermal patch will be applied for 12 hours per day (overnight) to avoid development of nitrate tolerance. Energy expenditure will be measured by indirect calorimetry before and after a mild cold exposure by placing a medical cooling system around their midsection. Additionally brown adipose tissue activity will determined by 18F-FDG-PET/CT 30 min after injection of 75 MBq of 18F-FDG. To analyse changes in BAT and muscle which are caused by glyceroltrinitrate as compared with cold stimulus, a tissue biopsie of supraclavicular BAT and skeletal muscle (musculus vastus lateralis) will be performed. To measure changes in glucose tolerance and triglyceride levels a mixed meal test will be performed during control and treatment phase respectively. Additionally, a indirect calorimetry will be conducted before and after mixed meal test to assess diet-induced thermogenesis, i.e. the increase in REE due to ingestion of nutrients In control phase the same examinations will be performed, but without glyceroltrinitrat.


Recruitment information / eligibility

Status Recruiting
Enrollment 25
Est. completion date August 31, 2025
Est. primary completion date May 31, 2025
Accepts healthy volunteers Accepts Healthy Volunteers
Gender All
Age group 18 Years to 40 Years
Eligibility Inclusion Criteria: - BMI 18.5 to 25 kg/m2 or 30 to 35kg/m2 - Able to give informed consent as documented by signature - Age 18 to 40 years Exclusion Criteria: - Contraindications to glyceroltrinitrate, e.g. known hypersensitivity or allergy - Concomitant medication: Non-steroidal anti-inflammatory drugs (NSAID), glucocorticoids, diuretics, antihypertensives, fibrates or statins, metformin, PDE-5-inhibitors - Clinically significant concomitant disease states (e.g. renal failure, hepatic dysfunction, cardiovascular disease, diabetes mellitus) - Hypersensitivity to cold (e.g. Raynaud Syndrome) - Orthostatic hypotension: systolic blood pressure decreases min. 20mmHg or diastolic blood pressure decreases min 10mmHg within 3-5min after standing up (Schellong Test at screening visit) - History of orthostatic syncope or pre-syncope - Blood pressure values below 100 mmHg systolic and 60 mmHg diastolic - Allergy to local anesthetic - Hypothyroidism without sufficient substitution - Hyperthyroidism - Claustrophobia - Smoker / habitual tobacco use - Habitual excessive alcohol use - Weight change of >5% within 3 months prior to inclusion - Known or suspected non-compliance, drug or alcohol abuse - Inability to follow the procedures of the study, e.g. due to language problems, psychological disorders, dementia, etc. of the participant - Enrolment of the investigator, his/her family members, employees and other dependent persons - Enrolment into another study using ionizing radiation within the previous 12 months - Pregnant or lactating women - Lab parameters - Hb below lower reference limit - Glycated Hemoglobin (HbA1c): above 6.0% - Random plasma glucose >11 mM

Study Design


Related Conditions & MeSH terms


Intervention

Drug:
Glyceroltrinitrat
transdermal patch

Locations

Country Name City State
Switzerland University Hospital Basel, Department of Endocrinology Basel BS

Sponsors (1)

Lead Sponsor Collaborator
University Hospital, Basel, Switzerland

Country where clinical trial is conducted

Switzerland, 

Outcome

Type Measure Description Time frame Safety issue
Other Tissue biopsies of supraclavicular BAT and skeletal muscle musculus vastus lateralis Analysis of the transcriptional changes both in BAT and muscle which are caused by the administration of glyceroltrinitrate (Nitroderm® TTS) as compared with no intervention After 14 days treatment with glyceroltrinitrate or after 14 days control period respectively
Primary BAT SUVmean 18F-FDG uptake into the supra-clavicular brown adipose tissue as determined by 18F- After 14 days treatment with glyceroltrinitrate or after 14 days control period respectively
Primary BAT SUVmax maximum SUV in supraclavicular adipose tissue (according to BARCIST 1.0) After 14 days treatment with glyceroltrinitrate or after 14 days control period respectively
Secondary BAT Volume volume of supraclavicular adipose tissue (according to BARCIST 1.0) After 14 days treatment with glyceroltrinitrate or after 14 days control period respectively
Secondary BAT glycolytic volume volume of supraclavicular adipose tissue x SUVmean (according to BARCIST 1.0) After 14 days treatment with glyceroltrinitrate or after 14 days control period respectively
Secondary Energy expenditure Quantitative change in EE above resting metabolic rate in response to the respective study intervention. Measured by indirect calorimetry. After 14 days treatment with glyceroltrinitrate or after 14 days control period respectively
Secondary Cold induced thermogenesis rise in energy expenditure above baseline occurring during mild cold exposure After 14 days treatment with glyceroltrinitrate or after 14 days control period respectively
Secondary Mixed meal test Changes in glucose tolerance and triglyceride levels after a mixed meal test in the respective study visit. After 14 days treatment with glyceroltrinitrate or after 14 days control period respectively
Secondary Skin temperature - Changes in skin temperature and body core temperature during treatment with glyceroltrinitrate (Nitroderm® TTS) compared to control. After 14 days treatment with glyceroltrinitrate or after 14 days control period respectively
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