Biliary Stricture Clinical Trial
Official title:
Fluorescent in Situ Hybridization in Diagnosis of Biliary Stricture: A Feasibility Study
The management of biliary strictures depends on their correct pre-operative evaluation which remains challenging. Despite the emerging multitudes of new diagnostic opportunities- modalities we have today, there is still a large number of biliary stenosis misdiagnosed with a profound negative impact on the patients´ outcome. The study aims to proove the feasibility and to evaluate the impact of Fluorescent In Situ Hybridization (FISH) on the tissue diagnostic of biliary strictures.
The management of biliary strictures depends on their correct pre-operative evaluation which
remains challenging. Biliary strictures have various etiologies (traumatic, inflammatory,
tumoral, ischemic etc), which are necessarily needed to be known for the correct therapeutic
approach. Despite the emerging multitudes of new diagnostic opportunities, there is still a
large number of biliary stenosis misdiagnosed with a profound negative impact on the
patients´ outcome. The dilemma that exists is how to balance the risk of missing the chance
of curative surgery for some malignancy and preventing some patients from unnecessary surgery
for benign etiologies and not to waste time. Different conventionnal sampling methods (as
Brush-cytology, forceps biopsies during ERCP, endoscopic guided fine needle
aspiration-EUS-FNA) have relatively low sensitivity. In such cases, the peroral
cholangioscopy proves diagnostic accuracy of 90 %. This method remains expert dependent,
costly and may be result in complications of cholangitis in 3-5 % of cases. Techniques or
others methods less complicated and improving the preoperative diagnosis of biliary
strictures are needed. Fluorescent in Situ Hybridization (FISH) was shown to improve the
diagnostic yield of routine cytology.
This study will proove the feasebility and the clinical place of FISH in the diagnostic of
biliary strictures and evaluate the impact of FISH on management of patients with biliary
strictures.
FISH (Fluorecent In Situ Hybridization) is a molecular cytogenetic method, which enables the
detection of fluorescently- labeled DNA/RNA or oligonucleotide probes hybridized to
metaphase/ interphase. It uses probes to bind to specific DNA/RNA sequences. This enables the
detection of aneuploidy for chromosomes 3, 7, 17 and loss of the 9p21 in patients with
suspected malignant biliary strictures.
Different methods were used to take samples from the site of the stenosis. Brush-cytology and
endocanal forceps biopsies during ERCP and FNA (fine needle aspiration) during EUS
(endosonography). These sampling techniques have relatively low specificity and sensitivity.
Reason why we will combine FISH with the sampling methods to maximize our chance to early
determine the etiology of stenosis and avoid wasting time and unnecessary cholangioscopy. In
this study, the positivity of FISH for Chromosomes 3,7,17 is defined by a presence of
polysomy of these chromosomes and the positivity of FISH for Chromosomal region defined by a
presence of heterozygous delection or homozygous delection for 9p21. Polysomy is defined by a
gain of 2 or more chromosomes in 4 cells. For the chromosomal region, the delection or loss
of 9p21 must be observed in 12 cells.
Methods:
- Tissue specimens obtained via either brush cytology, forceps biopsy or fine needle
aspiration during ERCP or endosonography (EUS) were examined by routine cytology or
histology (RC) methods.
- In addition, FISH using fluorescent-based polynucleotide probes targeting chromosomes 3,
7, 17 and locus 9p21 was performed (ZytoVysion®).
Success (positivity) is defined by the presence of polysomy for chromosomes 3, 7, 17 and/or
the presence of delection or loss of the chromosomal region 9p21 in patients with suspected
malignant biliary strictures.
Gold standard for final diagnosis should be the histology from surgery resection. In patients
without surgery, a follow up of 12 months will be considered adequate to exclude or confirm
malignant etiology of the stritures.
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