Acute Lung Injury Clinical Trial
Official title:
the Change of R-spondin Proteins Plasma Concentration Level Caused by Mechanical Ventilation and Its Effect on Mechanical Ventilation Induced Lung Injury
Verified date | October 2017 |
Source | Xinhua Hospital, Shanghai Jiao Tong University School of Medicine |
Contact | n/a |
Is FDA regulated | No |
Health authority | |
Study type | Observational |
As novel agonists of Wnt/β-catenin signaling pathway, R-spondin proteins constitute a class of ligands, including R-spondin 1/2/3/4, functioning through their receptors leucine-rich repeat-containing G-protein coupled receptor (LGR)4/5/6 to enhance Wnt/β-catenin activity. Since Wnt signaling plays pivotal roles in the regulation of many life processes involved in embryogenesis and adulthood, R-spondin proteins also take part in cell proliferation, differentiation and morphogenesis.For example, in the formation of respiratory system,R-spondin 2 is required for normal laryngeal-tracheal and lung morphogenesis,and the lack of R-spondin 1 expression results in the absence of duct side-branching development and subsequent alveolar formation. In addition, R-spondins show protective effect in tissue injury and diseases. R-spondin 1 and R-spondin 3 have been reported to prevent chemotherapy- or radiotherapy-induced mucous membrane lesion. R-spondin 1 attenuates oral mucositis contributed by radiotherapy in mouse models and R-spondin 3 potentiates intestinal regeneration elicited via gastrointestinal toxic effect of chemoradiotherapy treatment. However, whether R-spondin proteins exert salient influence on acute lung injury especially induced by mechanical ventilation is deficient. Therefore, this study aims to ascertain the implication of R-spondin proteins in the pathology of mechanical ventilation induced lung injury through detecting human plasma concentration change of R-spondin 1/2/3/4 after mechanical ventilation and interference effects in mouse model, which is helpful for prevention and treatment of ventilation induced lung injury.
Status | Completed |
Enrollment | 52 |
Est. completion date | October 31, 2017 |
Est. primary completion date | October 15, 2017 |
Accepts healthy volunteers | No |
Gender | All |
Age group | 18 Years to 60 Years |
Eligibility |
Inclusion Criteria: - undergo elective surgery with mechanical ventilation lasting for > 3 hours; classified as physical status I to III according to the American Society of Anesthesiologists Physical Status Classification System; Written informed consent is approved. Exclusion Criteria: - chronic lung disease; recent lung infection; recent anaesthetics or mechanical ventilation treatment; hemodilution with massive fluid supply during surgery; children;women during pregnancy or lactation; being involved in other clinical subjects |
Country | Name | City | State |
---|---|---|---|
China | Department of Anesthesia, Shanghai Xinhua hospital | Shanghai | Shanghai |
Lead Sponsor | Collaborator |
---|---|
Xinhua Hospital, Shanghai Jiao Tong University School of Medicine |
China,
Type | Measure | Description | Time frame | Safety issue |
---|---|---|---|---|
Primary | Change in Plasma Concentration of R-spondin 1 | The venous blood samples were collected twice for each patient that the first time was around the onset of the mechanical ventilation and the second was 3rd hour after the onset of the mechanical ventilation, which were named as sample A and sample B relatively. Then, plasmids were separated by centrifugation and detected for R-spondin1 concentration. And the outcome was calculated by subtracting the R-spondin1 plasmid concentration of sample A from the R-spondin1 plasmid concentration of sample B, which was the change in plasma concentration of R-spondin1. | 3 hours | |
Primary | Change in Plasma Concentration of R-spondin 2 | The venous blood samples were collected twice for each patient that the first time was around the onset of the mechanical ventilation and the second was 3rd hour after the onset of the mechanical ventilation, which were named as sample A and sample B relatively. Then, plasmids were separated by centrifugation and detected for R-spondin2 concentration. And the outcome was calculated by subtracting the R-spondin2 plasmid concentration of sample A from the R-spondin2 plasmid concentration of sample B, which was the change in plasma concentration of R-spondin2. | 3 hours | |
Primary | Change in Plasma Concentration of R-spondin3 | The venous blood samples were collected twice for each patient that the first time was around the onset of the mechanical ventilation and the second was 3rd hour after the onset of the mechanical ventilation, which were named as sample A and sample B relatively. Then, plasmids were separated by centrifugation and detected for R-spondin3 concentration. And the outcome was calculated by subtracting the R-spondin3 plasmid concentration of sample A from the R-spondin3 plasmid concentration of sample B, which was the change in plasma concentration of R-spondin3. | 3 hours | |
Primary | Change in Plasma Concentration of R-spondin4 | The venous blood samples were collected twice for each patient that the first time was around the onset of the mechanical ventilation and the second was 3rd hour after the onset of the mechanical ventilation, which were named as sample A and sample B relatively. Then, plasmids were separated by centrifugation and detected for R-spondin4 concentration. And the outcome was calculated by subtracting the R-spondin4 plasmid concentration of sample A from the R-spondin4 plasmid concentration of sample B, which was the change in plasma concentration of R-spondin4. | 3 hours |
Status | Clinical Trial | Phase | |
---|---|---|---|
Recruiting |
NCT03937947 -
Traumatic Brain Injury Associated Radiological DVT Incidence and Significance Study
|
||
Completed |
NCT04247477 -
Comparison of Different PEEP Titration Strategies Using Electrical Impedance Tomography in Patients With ARDS
|
N/A | |
Not yet recruiting |
NCT02693912 -
Changes in Alveolar Macrophage Function During Acute Lung Injury
|
N/A | |
Completed |
NCT01659307 -
The Effect of Aspirin on REducing iNflammation in Human in Vivo Model of Acute Lung Injury
|
Phase 2 | |
Completed |
NCT01552070 -
Recruitment on Extravascular Lung Water in Acute Respiratory Distress Syndrome (ARDS)
|
Phase 2 | |
Unknown status |
NCT01186874 -
Epidemiology Research on Acute Lung Injury/Acute Respiratory Distress Syndrome (ALI/ARDS) in Adult ICU in Shanghai
|
N/A | |
Withdrawn |
NCT00961168 -
Work of Breathing and Mechanical Ventilation in Acute Lung Injury
|
N/A | |
Recruiting |
NCT00759590 -
Comparison of Two Methods to Estimate the Lung Recruitment
|
N/A | |
Completed |
NCT00736892 -
Incidence of Acute Lung Injury: The Alien Study
|
||
Completed |
NCT02475694 -
Acute Lung Injury After Cardiac Surgery: Pathogenesis
|
N/A | |
Completed |
NCT00825357 -
Biological Markers to Identify Early Sepsis and Acute Lung Injury
|
N/A | |
Terminated |
NCT00263146 -
Recruitment Maneuvers in ARDS: Effects on Respiratory Function and Inflammatory Markers.
|
N/A | |
Completed |
NCT00188058 -
Comparison of 2 Strategies of Adjustment of Mechanical Ventilation in Patients With Acute Respiratory Distress Syndrome
|
N/A | |
Completed |
NCT00234767 -
Study of the Economics of Pulmonary Artery Catheter Use in Patients With Acute Respiratory Distress Syndrome (ARDS)
|
Phase 3 | |
Recruiting |
NCT02598648 -
Role and Molecular Mechanism of Farnesoid X Receptor(FXR) and RIPK3 in the Formation of Acute Respiratory Distress Syndrome in Neonates
|
N/A | |
Recruiting |
NCT02948530 -
Measurement of Lung Elastance and Transpulmonary Pressure Using Two Different Methods (Lungbarometry)
|
||
Completed |
NCT01532024 -
Exploratory Clinical Study of Neutrophil Activation Probe (NAP) for Optical Molecular Imaging in Human Lungs
|
Early Phase 1 | |
Recruiting |
NCT01992237 -
Measuring Energy Expenditure in ECMO (Extracorporeal Membrane Oxygenation) Patients
|
N/A | |
Completed |
NCT01486342 -
PET Imaging in Patients at Risk for Acute Lung Injury
|
N/A | |
Completed |
NCT01321398 -
Transpulmonary Pressure Gradients in High Frequency Oscillation
|
N/A |