Probiotics, Respiratory and Intestinal Microbiome and Respiratory Tract Infections in Children

Respiratory Infections in Children Clinical Trial

Official title:

The Impact of Probiotics in the Respiratory and Gastrointestinal Microbiome and Its Role in Respiratory Tract Infections in Children

Clinical Trial Details — Status: Recruiting

Administrative data

• NCT number: NCT03683927
• Other study ID #: CONACYT PN 2015-01-878
• Status: Recruiting
• Phase: Phase 1
• Start date: January 10, 2018
• Est. completion date: March 10, 2020

Study information

• Verified date: September 2018
• Source: Universidad Nacional Autonoma de Mexico
• Contact: Rosa M Wong-Chew, MD, DSc
• Phone: 5255 56232300
• Email: rmwong[@]unam.mx
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

Pneumonia and diarrhea are the most frequent causes of infectious diseases in children under 5 years of age worldwide, responsible for 1.5 million deaths annually.

In up to 80% of pneumonia cases the etiology is viral. Some viruses can persist up to 6 months after an acute infection. The time when viruses enter the body and whether they are commensals or only cause disease and are eliminated after an acute infection is unknown.

Modern techniques have identified diverse communities of microbiota in healthy and sick people, and viral communities associated in a close interaction. The acquisition and colonization by respiratory viruses and the role in health and disease in this niche that is the microbiome is unknown.

The role of probiotics in the prevention of respiratory disease and in the maintenance of homeostasis in the microbiota is poorly understood, and even more the probable relationship between the microbiota, the respiratory viruses that could be commensals or pathogens at the respiratory level, the time when children can be colonized, and their regulation with the administration of probiotics.

The aims of the study are to determine the changes in the intestinal and respiratory microbiota, the viruses that can be commensals or cause disease and the role of probiotics in the prevention of respiratory diseases during the first year of life.

A prospective, randomized, controlled clinical trial will be carried out making basic metagenomics studies (translational medicine). After informed consent, 120 newborns will be randomized into 2 groups, one will receive probiotics 4 times a week orally and the other a placebo consisting of sterile water 4 times a week. The clinical follow up will be done every 2 months until 1 year old, nasal washes and stool samples will be collected to determine the intestinal and respiratory microbiome. Multiplex polymerase chain reaction studies will be conducted to detect the presence of respiratory viruses and the time when the children acquire viruses that are commensal or only in the case of respiratory infection. The mothers will be asked to come for consult in case of respiratory infection and a nasal wash and stool sample will be taken. Descriptive, bivariate and multivariate statistics will be used to determine the associations between the microbiota, the viral metagenomics, the respiratory viruses and the risk of presenting or not respiratory infection in the group receiving probiotics compared to placebo.

Description:

Pneumonia and diarrhea are the 2 most frequent causes of infectious diseases in children under 5 years of age worldwide, responsible for 1.5 million deaths annually. In 2013, they corresponded to 15% and 9%, respectively, of the 6.3 million deaths in children under 5 years of age. In our country according to the Ministry of Health, acute respiratory infections are the leading cause of disease nationwide with 27,493, 239 cases reported in 2014 in which pneumonia ranks 19th in the top 20 causes of disease with 174,748 cases, of which 51,893 cases (29%) are in children under 5 years of age, and 21,404 cases (12%) in children under 1 year old; in 2015, 148,140 cases of pneumonia were reported.

It is estimated that in up to 80% of pneumonia cases the etiology is viral. It has been reported that some viruses such as adenovirus or bocavirus can persist up to 6 months after an acute infection. The time when viruses enter the body and whether they are commensals of the respiratory tract or only cause disease and are eliminated after an acute infection is unknown.

Modern techniques have identified diverse communities of microbiota in healthy and sick people, and viral communities associated in a close interaction, which has changed concepts of pathogenesis of respiratory tract infections. The interaction of respiratory viruses, the acquisition and colonization by respiratory viruses and the role in health and disease in this niche that is the microbiome is not known.

The role of probiotics in the prevention of respiratory disease and in the maintenance of homeostasis in the microbiota is poorly studied, and even more the probable relationship between the microbiota, the viroma, the respiratory viruses that could be commensals or pathogens at the respiratory level, the time when children can be colonized, and their regulation with the administration of probiotics.

The aims of the study are to determine the changes in the intestinal and respiratory microbiota, the intestinal and respiratory viroma, the viruses that can be commensals or cause disease and the role of probiotics in the prevention of respiratory diseases during the first year of life.

Methods A prospective, randomized, controlled clinical trial will be carried out making basic metagenomics studies applied to the clinic (translational medicine). In this study the investigators plan to follow up a cohort of 120 children from newborns to one year of age. After informed consent, children will be randomized into 2 groups, one will receive probiotics 4 times a week orally and the other a placebo consisting of sterile water 4 times a week. The clinical follow up will be done every 2 months, where nasal washes and stool samples will be collected to determine the intestinal and respiratory microbiome and the viral metagenomics. Multiplex polymerase chain reaction studies will be conducted to detect the presence of respiratory viruses and detect the time when the children acquire viruses that are commensal or only in case of respiratory infection with manifestations of disease. The mothers will document and will be asked to come for consult in case of respiratory infection, in which samples of nasal washes will be taken to determine the virus or bacteria that could be causing the infection. Descriptive, bivariate and multivariate statistics will be used to determine the associations between the microbiota, the viral metagenomics, the respiratory viruses and the risk of presenting or not respiratory infection in the group receiving probiotics compared to placebo. A p< 0.05 will be considered statistically significant.

Recruitment information / eligibility

• Status: Recruiting
• Enrollment: 120
• Est. completion date: March 10, 2020
• Est. primary completion date: January 9, 2020
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: N/A to 2 Days

Eligibility

Inclusion Criteria:

• Heathy term newborns

• Vaginal or cesarean section delivery

• Informed consent of both parents to participate

Exclusion Criteria:

• Preterm newborns

• Co-morbidities

Related Conditions & MeSH terms

• Communicable Diseases
• Infection
• Respiratory Infections in Children
• Respiratory Tract Infections

Intervention

Combination Product:
• Bacillus clausii
The probiotic (Bacillus clausii) will be administered 4 times a week to the infant

Other:
• Sterile water
The placebo group will receive sterile water 4 times a week

Locations

Country	Name	City	State
Mexico	Rosa Maria Wong-Chew	Mexico city

Sponsors (2)

Lead Sponsor	Collaborator
Universidad Nacional Autonoma de Mexico	Hospital General de Mexico

Country where clinical trial is conducted

Mexico, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Number of respiratory infections in the first year of life	Follow up of the infants will be performed every 2 months during 1 year to look at the incidence of respiratory tract infections in each arm	1 year
Secondary	Rates of bacterial phyla in nasal and intestinal washes	Nasal washes and stool samples will be taken and analyzed for the microbiome. The proportion of phyla will be compared between the probiotic and placebo groups.	1 year
Secondary	Number of viruses detected in nasal washes during the follow up	Nasal washes will be taken every 2 months during 1 year to detect by multiplex polymerase chain reaction viruses that could be in the nasopharynx of the children	1 year