Biomarkers of Meats and Potatoes Intake

Nutrition Disorders Clinical Trial

— MEPO  

Official title:

Biomarkers of Meats and Potatoes Intake: a Meal Study in Healthy Men and Women - the MEPO Study

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT04548362
• Other study ID #: H-150204011H
• Status: Completed
• Phase: N/A
• Start date: February 15, 2016
• Est. completion date: May 30, 2016

Study information

• Verified date: April 2021
• Source: University of Copenhagen
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

This cross-over meal study is a four-way intervention with 12 volunteers randomized at the same time to four meals with fried meats (beef, pork, chicken and amino-acid matched non-meat control) and to four meals with potato products (boiled, chips, fries, and a control with boiled white rice) to search for biomarkers of intake.

Description:

In a two-dimensional, cross-over meal study, 12 healthy volunteers consumed in a randomized sequence four test meals: chicken, pork, beef, and a control made of egg white and pea. At the same time they were independently randomized to four starchy foods to be consumed together with the meats: boiled potatoes, fried potatoes, potato crisps or boiled white rice. Fasting and postprandial urine samples were collected to cover 48 h after each meal and samples were profiled by untargeted LC-ESI-qTOF-MS metabolomics. The profiles following the meal challenges were explored by univariate and multivariate analyses and single and combined markers of intake identified chemically by MS/MS fragmentation experiments and statistically by ROC curves and error rates as well as FDR statistics.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 12
• Est. completion date: May 30, 2016
• Est. primary completion date: May 30, 2016
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 18 Years to 70 Years

Eligibility

Inclusion Criteria:

• healthy

Exclusion Criteria:

• non-pregnant, no abuse of alcohol or drugs, no chronic medication except p-pills

Related Conditions & MeSH terms

• Nutrition Disorders

Intervention

Other:
• Meat arm
Randomised sequence of pork, beef, chicken meat, and an egg white/pea combination
• Starchy foods arm
Randomised sequence of boiled potato, fried potato, potato crisps, and boiled white rice

Locations

Country	Name	City	State
Denmark	Lars Dragsted	Frederiksberg

Sponsors (1)

Lead Sponsor	Collaborator
Professor Lars Ove Dragsted

Country where clinical trial is conducted

Denmark, 

References & Publications (2)

Cuparencu C, Rinnan Å, Dragsted LO. Combined Markers to Assess Meat Intake-Human Metabolomic Studies of Discovery and Validation. Mol Nutr Food Res. 2019 Sep;63(17):e1900106. doi: 10.1002/mnfr.201900106. Epub 2019 Jun 13. — View Citation

Zhou X, Ulaszewska MM, Cuparencu C, De Gobba C, Vázquez-Manjarrez N, Gürdeniz G, Chen J, Mattivi F, Dragsted LO. Urine Metabolome Profiling Reveals Imprints of Food Heating Processes after Dietary Intervention with Differently Cooked Potatoes. J Agric Food Chem. 2020 Jun 3;68(22):6122-6131. doi: 10.1021/acs.jafc.0c01136. Epub 2020 May 8. — View Citation

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	meat intake biomarkers	To identify intake biomarkers of general meat, red meat and poultry	0-48 hours
Primary	Potato intake biomarkers	To identify intake biomarkers of general potato, fried potato and white rice	0-48 hours