The Effects of Dietary Nitric Oxide and Anthocyanins on Anaerobic Exercise Performance

Exercise Clinical Trial

Official title:

The Effects of Dietary Nitric Oxide and Anthocyanins on Anaerobic Exercise Performance

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT03631394
• Other study ID #: 5180190
• Status: Completed
• Phase: N/A
• Start date: September 1, 2018
• Est. completion date: April 1, 2019

Study information

• Verified date: May 2019
• Source: Loma Linda University
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

The purpose of this graduate student research study is to investigate the effects of nitrate from beetroot powder with additional anthocyanins from tart cherry on exercise performance in healthy Loma Linda University students utilizing the Wingate Anaerobic test on stationary bike.

Description:

A randomized controlled double-blind crossover study with a baseline and two interventions. On day 1 (baseline), all subjects will have a lactate fingerstick measurement before and after the Wingate protocol. A random subsample (1/3 of subjects) will have 10 milliliters (2 teaspoon) of blood drawn for determination of serum allantoin. Subjects will then be equipped with a pulse oximeter and a heart rate monitor after which they will perform the Wingate Anaerobic test protocol. The protocol includes: 4 minutes of warm up, 3 seconds of warm up sprint with weights, 1-minute relaxed cycling, 2-minute rest, then 10 seconds cycling without weights. This will be followed by 30 seconds sprint with weights and end with 2 minutes of relaxed cycling. A post-activity blood draw will be performed for another 10 milliliters (2 teaspoon) of blood from the same one-third of subjects previously sampled. After baseline data collection, subjects will undergo a 1-week washout (avoiding high antioxidant foods) after which they will be randomized to treatment groups. On day 9, subjects will begin supplementation with nitrate from beetroot powder with additional anthocyanins, or nitrate from beetroot powder with a placebo for 7 days. On day 15 the subject will again repeat the same procedures as day 1, followed by another week of washout. They will supplement again on day 23 and repeat the same procedure as the first intervention on day 29.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 28
• Est. completion date: April 1, 2019
• Est. primary completion date: April 1, 2019
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 21 Years to 35 Years

Eligibility

Inclusion Criteria:

• Healthy Loma Linda University students aged between 21 and 35 years old

• Willing to abstain from caffeine, mouthwash, chewing gums, and high antioxidant foods during the week of supplementation.

Exclusion Criteria:

• Diabetic

• having salivary gland disorder, cardiovascular disease or orthopedic injuries

• pregnant or breastfeeding

• taking NSAIDS

• have an allergy to anthocyanins and/or beetroot

• women who are on their menses

Related Conditions & MeSH terms

• Exercise

Intervention

Other:
• beetroot and anthocyanin
Beetroot and anthocyanin will be taken for 7 days during the study two hours prior to eating.
• beetroot and placebo
Beetroot and placebo will be taken for 7 days during the study two hours prior to eating.

Locations

Country	Name	City	State
United States	Loma Linda Universtiy	Loma Linda	California

Sponsors (1)

Lead Sponsor	Collaborator
Loma Linda University

Country where clinical trial is conducted

United States, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Change in blood lactate levels	Participants in this study should reach an anaerobic state as they perform the Wingate test. This lactate pro analyzer will be a tool to examine the participant's change in blood lactate levels by means of POC fingertip lactate measurement. Lactate levels will be measured in all participants at baseline, before and after Wingate Anaerobic test.	Day 1
Primary	Change in blood lactate levels	Participants in this study should reach an anaerobic state as they perform the Wingate test. This lactate pro analyzer will be a tool to examine participant's change in blood lactate levels by means of POC fingertip lactate measurement. Lactate levels will be measured in all participants at intervention I, before and after Wingate Anaerobic test.	Day 15
Primary	Change in blood lactate levels	Participants in this study should reach an anaerobic state as they perform the Wingate test. This lactate pro analyzer will be a tool to examine participant's change in blood lactate levels by means of POC fingertip lactate measurement. Lactate levels will be measured in all participants at intervention II, before and after Wingate Anaerobic test.	Day 29
Secondary	Allantoin blood level	Non-fasting blood tests will be done for allantoin during baseline, intervention I, and intervention II.	Baseline, day 15, and day 29