Defining the Role of the Gut Microbiome in Mediating the Effects of Obesity on Intestinal Stem Cells

Obesity Clinical Trial

Official title: Defining the Role of the Gut Microbiome in Mediating the Effects of Obesity on Intestinal Stem Cells

Status Terminated

Clinical Trial Summary

New studies are revealing how a high-fat diet could be making the cells of the intestinal lining more likely to become cancerous. The investigators would like to study how obesity influences growth of intestinal stem cells, which could then trigger intestinal tumors.

The investigators are proposing a Pilot Study of 20 subjects (comprised of 10 participants from each of two different BMI categories: 20-25 and 35 & above), who will be asked to provide blood, stool, & urine samples, undergo a flexible sigmoidoscopy, and complete food frequency questionnaires

Clinical Trial Description

Hypothesis:

The investigators will test the hypothesis that the gut microbiome drives many of the effects that a pro-obesity high-fat diet has on intestinal stem cell (ISC) biology, which includes 1) an increase in ISC numbers and proliferation, 2) a reduced dependency on niche cells for intestinal organoid initiation, or 3) the acquisition of organoid-initiation by non-stem cell population in the intestinal epithelium.

Specific Aims:

1. To determine whether differences in the gut microbiome in obese human subjects correlates with enhanced intestinal stem cell numbers and function.

2. To validate whether obesity-driven changes in the gut microbiome of human recapitulate the effects of obesity on intestinal stem cell function in germ-free mice. ;

Related Conditions & MeSH terms

• Obesity

• NCT number: NCT02863029
• Study type: Interventional
• Source: Mayo Clinic
• Status: Terminated
• Phase: N/A
• Start date: January 2017
• Completion date: March 9, 2017