SAW Lung Microbiome Study in Smokers and Never-smokers

Healthy Clinical Trial

Official title:

Leibniz Association Senate Board of Competition (SAW) Lung Microbiome Study

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT03562442
• Other study ID #: SAW Lungenmikrobiom-Studie
• Status: Completed
• Phase: N/A
• Start date: March 29, 2017
• Est. completion date: March 1, 2020

Study information

• Verified date: April 2024
• Source: Research Center Borstel
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

The trial aims to analyse changes in the microbiome of the lower airways after smoking cessation. Microbiome analyses (upper airway swabs, bronchoalveolar lavage, transbronchial brushing) are conducted in smokers before and 6 weeks after smoking cessation. Never smokers serve as a control group and undergo the same sampling procedures once.

Description:

The trial aims to analyze changes in the microbiome of the lower airways after smoking cessation. Microbiome analyses (upper airway swabs, bronchoalveolar lavage, transbronchial brushing) are conducted in healthy (and willing to quit) smokers before and 6 weeks after smoking cessation. Never smokers serve as a control group and undergo the same sampling procedures once.

Demographic and clinical data of the study subjects are obtained, aiming for homogeneous groups. All personal data are encrypted according to the standard operating procedures of the study site. Pharyngeal and deep nasal swabs are taken.

To avoid contamination of the lower airways from the oropharynx through the bronchoscope, standardized procedures are established according to the German guidelines for bronchoscopy. Before bronchoscopy, the endoscope is flushed with sterile saline which is analyzed for 16S ribosomal RNA (rRNA) to control for residual contaminating bacterial desoxyribonucleic acid (DNA). A 300ml bronchoalveolar lavage with warm normal saline solution is taken from the middle lobe. Three covered and "microbiologically protected" bronchial brushings are obtained from the right upper lobe. This sterile brush is sealed in wax and pushed through the wax just before taking the brushing. All samples are immediately frozen and stored at -80°C until further analyses. For cultivations, samples are kept at 4°C and rapidly processed.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 60
• Est. completion date: March 1, 2020
• Est. primary completion date: December 31, 2019
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 18 Years and older

Eligibility

Inclusion Criteria:

• Cigarette consumption of at least five cigarettes per day

• Caucasian ethnicity

Exclusion Criteria:

• Continous passive smoking exposure

• Systemic steroid therapy during the previous month

• Chronic lung disease (exception COPD stage Global Initiative on Lung Disease (GOLD) 0/I/II)

• Diabetes mellitus

• Pregnancy or breast feeding

• active tuberculosis (currently or previously)

• respiratory infection in the previous month

• antibiotic therapy in the previous two months

• immunosuppression

• malignancy

• interstitial lung disease

• rheumatic disease with lung manifestation

• member of a collaborative group of the project

Related Conditions & MeSH terms

• Chronic Obstructive Pulmonary Disease (COPD)
• Communicable Diseases
• Healthy
• Infections
• Lung Diseases, Obstructive
• Microbial Colonization
• Pulmonary Disease, Chronic Obstructive
• Smoking Cessation

Intervention

Behavioral:
• Smoking cessation
Smoking cessation with or without professional / medical support

Locations

Country	Name	City	State
Germany	Research Center Borstel	Borstel	Schleswig-Holstein

Sponsors (1)

Lead Sponsor	Collaborator
Research Center Borstel

Country where clinical trial is conducted

Germany, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Change in microbiome pattern in smokers after smoking cessation	Number of bacteria genera in the lung identified by 16S rRNA and bacterial culture	6 Weeks
Secondary	Smoking cessation rates	Percentage of sustained smoking cessation	6 weeks
Secondary	Smoking cessation rates	Percentage of sustained smoking cessation	6 months
Secondary	Lung microbiome in never-smokers	Number of bacteria genera in the lung identified by 16S rRNA and bacterial culture	0 days