Healthy Clinical Trial
Official title:
Glutamate Supplementation and Its Effects on Skeletal Muscle Glucose Metabolism During an Oral Glucose Challenge in Healthy Young Men
Verified date | June 2019 |
Source | University of Waterloo |
Contact | n/a |
Is FDA regulated | No |
Health authority | |
Study type | Interventional |
Amino acids play an important role in human metabolism. In aging individuals and in some diseases, certain amino acids, such as glutamate, are at lower than normal levels. Glutamate appears to be involved in providing energy and maintaining normal blood sugar (glucose) levels, processes which both depend heavily on skeletal muscle. The maintenance of healthy blood sugar levels, in particular, is tightly related to overall muscle mass and quality. To better understand the link between glutamate and glucose metabolism in skeletal muscle, the investigators will be using a nutritional approach to raise the body's glutamate levels with monosodium glutamate (MSG) supplementation, and raise blood glucose levels with a sugary drink. By altering the normal levels of glutamate and glucose in the blood and muscle tissue, the investigators can gather more information about the role of glutamate in energy metabolism. This will help the design of future studies investigating the function of glutamate in aging and disease. During this study the investigators will increase the levels of glutamate and glucose in the bloodstream by asking participants to ingest MSG along with a sugary drink. The goal is to subsequently determine: A) the amount of glutamate and glucose that ends up in the muscle; and B) whether normal skeletal muscle glucose metabolism, and the behaviour of additional amino acids (other than glutamate) is altered. The hypothesis is that when MSG and sugar drink are ingested together glucose uptake and metabolism within the skeletal muscle will be elevated.
Status | Completed |
Enrollment | 11 |
Est. completion date | October 2, 2018 |
Est. primary completion date | October 2, 2018 |
Accepts healthy volunteers | Accepts Healthy Volunteers |
Gender | Male |
Age group | 18 Years to 35 Years |
Eligibility |
Inclusion Criteria: - BMI in the normal or overweight range (18.5-30.0 kg/m2) - Weight stable for the past 6 months - Participate in aerobic and/or resistance-type exercise 3-5 times per week (no more then 2 hours per session and/or 5 sessions per week) - Fasting blood glucose < 6.0 mM - Resting blood pressure < 140/90 mmHg - Answer "no" to all questions on the Get Active Questionnaire (GAQ) Exclusion Criteria: - Smoking - Known allergy or intolerance to MSG - Diabetes, cancer, or other metabolic disorders - Cardiac or gastrointestinal problems - Infectious disease - Injuries that prevent safe participation and exercise or instructions from healthcare provider to refrain from exercise - Barium swallow or nuclear medicine scan in the previous 3 weeks - If receiving a DXA scan will cause the participant to exceed the maximum trivial dose of radiation per year - Prescription anti-coagulant or anti-platelet medication (e.g. warfarin, heparin, clopiodogrel) |
Country | Name | City | State |
---|---|---|---|
Canada | University of Waterloo | Waterloo | Ontario |
Lead Sponsor | Collaborator |
---|---|
University of Waterloo |
Canada,
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Chevassus H, Renard E, Bertrand G, Mourand I, Puech R, Molinier N, Bockaert J, Petit P, Bringer J. Effects of oral monosodium (L)-glutamate on insulin secretion and glucose tolerance in healthy volunteers. Br J Clin Pharmacol. 2002 Jun;53(6):641-3. — View Citation
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Di Sebastiano KM, Bell KE, Barnes T, Weeraratne A, Premji T, Mourtzakis M. Glutamate supplementation is associated with improved glucose metabolism following carbohydrate ingestion in healthy males. Br J Nutr. 2013 Dec;110(12):2165-72. doi: 10.1017/S0007114513001633. Epub 2013 Jun 11. — View Citation
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* Note: There are 12 references in all — Click here to view all references
Type | Measure | Description | Time frame | Safety issue |
---|---|---|---|---|
Primary | Glutamate | Circulating and intramuscular concentrations by HPLC | ~ 2 hours post-ingestion | |
Secondary | Glucose | Circulating concentrations using a spectrophotometric assay | ~ 2 hours post-ingestion | |
Secondary | Skeletal muscle glucose uptake | As indicated by GLUT4 and laminin co-localization (immunohistochemistry) | ~ 2 hours post-ingestion | |
Secondary | Insulin | Circulating concentrations using radioimmunoassay | ~ 2 hours post-ingestion | |
Secondary | C-peptide | Circulating concentrations using radioimmunoassay | ~ 2 hours post-ingestion | |
Secondary | Aspartate | Circulating and intramuscular concentrations by HPLC | ~ 2 hours post-ingestion |
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