COLON-IM : Microbiota and Immune Infiltrate in Normal, Dysplastic and Neoplastic Colorectal Tissue

Colorectal Cancer Clinical Trial

Official title:

COLON-IM : Prospective Cohort Study About Colorectal Environment : Microbiota and Immune Infiltrate in Normal, Dysplastic and Neoplastic Colorectal Tissue

Clinical Trial Details — Status: Recruiting

Administrative data

• NCT number: NCT03841799
• Other study ID #: ET18-276 (COLON-IM)
• Status: Recruiting
• Start date: May 2, 2019
• Est. completion date: December 2026

Study information

• Verified date: February 2023
• Source: Centre Leon Berard
• Contact: Gwenaelle GARIN
• Phone: 04 26 55 68 24
• Email: gwenaelle.garin[@]lyon.unicancer.fr
• Is FDA regulated: No
• Study type: Observational

Clinical Trial Summary

The primary objective of COLON-IM is to describe colorectal tissue microenvironment (neutrophils infiltrate) of patients with benign or malignant colorectal lesion (from stage I to III according to Tumor Node Metastasis (TNM)/ Union for International Cancer Control (UICC) classification).

Description:

Colorectal cancers (CRC) are the most common gastrointestinal cancers in Western countries (both Europe and the US) ; they are both associated with significant morbidity and mortality. The 5-year survival is around 63.5% (including all stages). Sporadic colon cancers make up 85% of all colorectal adenocarcinoma. Preneoplastic lesions accumulate alterations in genes that regulate cell growth. Patients suffering from CRC have a modified intestinal flora compared to healthy people. A specified microbiota profiled combined to genetic and immunological characteristics should be involved in colorectal carcinogenesis. Recent data have showed that responses to immunotherapies are associated with presence of certain bacteria in intestinal flora which potentially boost immune antitumor response. Immunotherapy is highly efficient in tumors with high mutation load and Microsatellite Instability-High (MSI-H). MSI-H tumors represent 15% of local colorectal tumors. Today, it is important to understand all immune agents roles in colorectal carcinogenesis in order to describe new interesting immune checkpoints to target. Tumor cells and tissues can escape immune surveillance and immune defense by several mechanisms. All immune cells subtypes present in the tumor, at the invasive tumor front and/or in tertiary lymphoid structures constitute the "immune context". The immune microenvironment has been shown to play a crucial role in disease progression, maintenance and resistance to therapy. All immune cells could play a pro-tumor or an anti-tumor role. For example, lymphocyte infiltrate is described as a prognostic factor in colorectal cancer : a tool (Immunoscore) has been validated as prognostic tool and it provides independent and superior prognostic value to TNM classification. This immune classification measures the host immune response at the tumor site and helps to guide treatment strategies. Adaptative immunity is well known ; however, innate immunity should also play an early role during carcinogenesis. Role of neutrophils in the tumor microenvironment remains controversial, with evidence for both pro- and anti-tumor roles.Tumour-associated neutrophils (TANS) have an antitumorigenic in early stage of carcinogenesis or a protumorigenic functions in metastatic stage. Evidences indicate that neutrophils manifest functional plasticity during oncogenic process. Neutrophil-to-lymphocyte ratio is associated with patient prognosis ; a high ratio seems to be associated with poor prognostic. Intestinal microbiota and immune infiltrate play key roles in colorectal microenvironment. Their interdependent interaction and their impacts on colorectal neoplasia and treatments are not well known. The primary objective of COLON-IM is to describe colorectal tissue microenvironment of patients with benign or malignant colorectal lesion (from stage I to III according to TNM/UICC classification).

Recruitment information / eligibility

• Status: Recruiting
• Enrollment: 80
• Est. completion date: December 2026
• Est. primary completion date: December 2024
• Accepts healthy volunteers: No
• Gender: All
• Age group: 18 Years and older

Eligibility

Inclusion Criteria:

• I1. Male or female patient 18 age or older at time of inform consent signature.
• I2. Patient with benign or malignant colorectal lesion (from stage I to III according to TNM/UICC classification) eligible to surgery, not previously be treated with an anticancer systemic agent (any type) and not be previously exposed to radiotherapy.
• I3. Patient should be able and willing to comply with procedures as per protocol.
• I4. Patient should understand, sign, and date the written voluntary informed consent form prior to any protocol-specific procedures performed.
• I5. Patient must be covered by a medical insurance.

Exclusion Criteria:

• E1. Pregnant or breast-feeding female patient.
• E2. Prior treatment with : Any immunomodulatory treatment (streroids, immunosuppressive therapies) within 4 weeks prior inclusion, Any antibiotics within 8 weeks prior inclusion.
• E3. Patients with secondary malignancy unless this malignancy is not expected to interfere with the evaluation of study endpoints and is approved by the sponsor. Examples of the latter include: in-situ carcinoma of the cervix treated adequately, basal or squamous cell carcinoma of the skin. Patients previously treated for another cancer type and without evidence of relapse for at least 1 year are eligible.
• E4. Patient with inflammatory disease or autoimmune disease.
• E5. Patient under curatorship, guardianship or judicial protection.

Related Conditions & MeSH terms

• Colorectal Cancer
• Colorectal Neoplasms

Locations

Country	Name	City	State
France	Centre Leon Berard	Lyon
France	GARBIT Vincent	Lyon

Sponsors (1)

Lead Sponsor	Collaborator
Centre Leon Berard

Country where clinical trial is conducted

France, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Characterization of colorectal tissue microenvironment of patients with locally colorectal neoplasia.	Rate of immune infiltrate in colorectal tissue	At surgery
Secondary	Transcriptome profiling by RNAseq of neutrophils associated with neoplastic and preneoplastic lesions.	mRNA level by RNAseq from neutrophils at different preneoplastic and neoplastic stages.	At surgery
Secondary	Characterization of lymphocyte cells infiltrate in colorectal tissue (normal, neoplastic and preneoplastic).	Rate of lymphocyte cells in colorectal tissue (normal, neoplastic and preneoplastic).	At surgery
Secondary	Evaluation of the correlation between immune infiltrate and clinical data, microsatellite status and clinical evolution.	Correlation between immune infiltrate (including neutrophils infiltrate) and clinical data (neoplastic lesion stage according to TNM/UICC classification and preneoplastic lesion (low or high dysplasia), microsatellite status, clinical outcome (survival without relapse and overall survival).	At surgery and through study completion, at least 42 months
Secondary	Evaluation of cytokinic environment associated to neoplastic and preneoplastic lesions and evaluation of the correlation between cytokinic environment and clinical data, microsatellite status and clinical evolution.	Correlation between cytokinic environment and clinical data (neoplastic lesion stage according to TNM/UICC classification and preneoplastic lesion (low or high dysplasia), microsatellite status, clinical outcome (survival without relapse and overall survival)	At surgery and through study completion, at least 42 months
Secondary	Identification of microbiota modifications in stools and mucosa during colic carcinogenesis	Sequences of the variable portions of the 16s ribosomal RNA gene	At surgery, at Month 6 post-surgery, at Month 9 post-surgery
Secondary	Identification of potential molecular abnormalities in cancer cells from colorectal tissue and from blood samples.	Circulating tumor DNA and tumor DNA.	At surgery